2.50
Hdl Handle:
http://hdl.handle.net/10541/91420
Title:
Characterization of epithelial cell activity in patients with oral cancer.
Authors:
Thomson, P J; Potten, Christopher S; Appleton, D R
Abstract:
Accurate, predictive assessment of the clinical behaviour and progression of individual oral cancers and premalignant lesions requires reproducible and quantitative analyses of diseased tissue. In this paper we describe the use of in vitro double labelling (sequential tritiated thymidine and bromodeoxyuridine staining of proliferating epithelial cells) to calculate S phase labelling indices (LIs), estimation of S phase duration (tS), and measurement of variables of flux to and from S for excised specimens of oral squamous cell carcinoma, premalignant lesions, and clinically normal mucosa from patients with oral cancer. There was a significant increase in mean LIs in buccal mucosa leukoplakias (14.5%) compared with normal mucosa (10.3%); P = 0.03. LIs were also increased in patients with cancers of the floor of mouth and ventral tongue but neither these changes nor alterations in flux parameters or S Phase durations were significant. Twenty-one kinetic profiles of dysplastic and malignant tissue were compared with conventional histopathological results, however, and these showed a 2.2% increase in LIs with each increase in grade of dysplasia (P = 0.004) and a 12% increase in LIs with each reduction in tumour differentiation (P = 0.02).
Affiliation:
Department of Oral & Maxillofacial Surgery, The Dental School, University of Newcastle upon Tyne, UK.
Citation:
Characterization of epithelial cell activity in patients with oral cancer. 1999, 37 (5):384-90 Br J Oral Maxillofac Surg
Journal:
The British Journal of Oral & Maxillofacial Surgery
Issue Date:
Oct-1999
URI:
http://hdl.handle.net/10541/91420
DOI:
10.1054/bjom.1999.0147
PubMed ID:
10577753
Type:
Article
Language:
en
ISSN:
0266-4356
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorThomson, P Jen
dc.contributor.authorPotten, Christopher Sen
dc.contributor.authorAppleton, D Ren
dc.date.accessioned2010-02-08T15:41:47Z-
dc.date.available2010-02-08T15:41:47Z-
dc.date.issued1999-10-
dc.identifier.citationCharacterization of epithelial cell activity in patients with oral cancer. 1999, 37 (5):384-90 Br J Oral Maxillofac Surgen
dc.identifier.issn0266-4356-
dc.identifier.pmid10577753-
dc.identifier.doi10.1054/bjom.1999.0147-
dc.identifier.urihttp://hdl.handle.net/10541/91420-
dc.description.abstractAccurate, predictive assessment of the clinical behaviour and progression of individual oral cancers and premalignant lesions requires reproducible and quantitative analyses of diseased tissue. In this paper we describe the use of in vitro double labelling (sequential tritiated thymidine and bromodeoxyuridine staining of proliferating epithelial cells) to calculate S phase labelling indices (LIs), estimation of S phase duration (tS), and measurement of variables of flux to and from S for excised specimens of oral squamous cell carcinoma, premalignant lesions, and clinically normal mucosa from patients with oral cancer. There was a significant increase in mean LIs in buccal mucosa leukoplakias (14.5%) compared with normal mucosa (10.3%); P = 0.03. LIs were also increased in patients with cancers of the floor of mouth and ventral tongue but neither these changes nor alterations in flux parameters or S Phase durations were significant. Twenty-one kinetic profiles of dysplastic and malignant tissue were compared with conventional histopathological results, however, and these showed a 2.2% increase in LIs with each increase in grade of dysplasia (P = 0.004) and a 12% increase in LIs with each reduction in tumour differentiation (P = 0.02).en
dc.language.isoenen
dc.subjectMouth Canceren
dc.subjectTongue Canceren
dc.subject.meshAnalysis of Variance-
dc.subject.meshAutoradiography-
dc.subject.meshCarcinoma, Squamous Cell-
dc.subject.meshCell Cycle-
dc.subject.meshCheek-
dc.subject.meshCytological Techniques-
dc.subject.meshEpithelial Cells-
dc.subject.meshHumans-
dc.subject.meshLeukoplakia, Oral-
dc.subject.meshMouth Floor-
dc.subject.meshMouth Mucosa-
dc.subject.meshMouth Neoplasms-
dc.subject.meshStaining and Labeling-
dc.subject.meshTongue Neoplasms-
dc.titleCharacterization of epithelial cell activity in patients with oral cancer.en
dc.typeArticleen
dc.contributor.departmentDepartment of Oral & Maxillofacial Surgery, The Dental School, University of Newcastle upon Tyne, UK.en
dc.identifier.journalThe British Journal of Oral & Maxillofacial Surgeryen

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