A high ratio of apoptosis to proliferation correlates with improved survival after radiotherapy for cervical adenocarcinoma.

2.50
Hdl Handle:
http://hdl.handle.net/10541/91382
Title:
A high ratio of apoptosis to proliferation correlates with improved survival after radiotherapy for cervical adenocarcinoma.
Authors:
Sheridan, Mary T; Cooper, Rachel A; West, Catharine M L
Abstract:
PURPOSE: A retrospective study was made of the role of apoptosis in determining radiotherapy outcome in 39 adenocarcinoma of the cervix. A comparison was also made of the detection of apoptosis by morphology and the TdT dUtp nick end-labeling (TUNEL) assay. METHODS AND MATERIALS: The level of apoptosis was assessed in paraffin-embedded sections by cell morphology, the TUNEL assay, and a combination of the two. A total of 2,000 cells were counted per section, to obtain apoptotic (AI) and mitotic (MI) indices. RESULTS: Patients with a high AI had a higher survival rate than those with a low AI, however, the difference was not significant. Using a ratio of apoptosis to proliferation indices, patients with an AI:MI > median had significantly better survival than those with AI:MI < median. This was true where the AI was quantified by morphology alone (p = 0.030) or in combination with the TUNEL assay (p = 0.008). Where the AI was quantified by a combination of morphology and TUNEL, the 5-year survival rates for women with AI:MI greater or less than the median were 81% and 25%, respectively. CONCLUSION: A high ratio of AI:MI in adenocarcinoma of the cervix indicates a good prognosis. A combination of the TUNEL assay and morphology provided the best discrimination between outcome groups.
Affiliation:
CRC Section of Genome Damage and Repair, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom.
Citation:
A high ratio of apoptosis to proliferation correlates with improved survival after radiotherapy for cervical adenocarcinoma. 1999, 44 (3):507-12 Int. J. Radiat. Oncol. Biol. Phys.
Journal:
International Journal of Radiation Oncology, Biology, Physics
Issue Date:
1-Jun-1999
URI:
http://hdl.handle.net/10541/91382
DOI:
10.1016/S0360-3016(99)00081-4
PubMed ID:
10348278
Type:
Article
Language:
en
ISSN:
0360-3016
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorSheridan, Mary Ten
dc.contributor.authorCooper, Rachel Aen
dc.contributor.authorWest, Catharine M Len
dc.date.accessioned2010-02-08T11:43:14Z-
dc.date.available2010-02-08T11:43:14Z-
dc.date.issued1999-06-01-
dc.identifier.citationA high ratio of apoptosis to proliferation correlates with improved survival after radiotherapy for cervical adenocarcinoma. 1999, 44 (3):507-12 Int. J. Radiat. Oncol. Biol. Phys.en
dc.identifier.issn0360-3016-
dc.identifier.pmid10348278-
dc.identifier.doi10.1016/S0360-3016(99)00081-4-
dc.identifier.urihttp://hdl.handle.net/10541/91382-
dc.description.abstractPURPOSE: A retrospective study was made of the role of apoptosis in determining radiotherapy outcome in 39 adenocarcinoma of the cervix. A comparison was also made of the detection of apoptosis by morphology and the TdT dUtp nick end-labeling (TUNEL) assay. METHODS AND MATERIALS: The level of apoptosis was assessed in paraffin-embedded sections by cell morphology, the TUNEL assay, and a combination of the two. A total of 2,000 cells were counted per section, to obtain apoptotic (AI) and mitotic (MI) indices. RESULTS: Patients with a high AI had a higher survival rate than those with a low AI, however, the difference was not significant. Using a ratio of apoptosis to proliferation indices, patients with an AI:MI > median had significantly better survival than those with AI:MI < median. This was true where the AI was quantified by morphology alone (p = 0.030) or in combination with the TUNEL assay (p = 0.008). Where the AI was quantified by a combination of morphology and TUNEL, the 5-year survival rates for women with AI:MI greater or less than the median were 81% and 25%, respectively. CONCLUSION: A high ratio of AI:MI in adenocarcinoma of the cervix indicates a good prognosis. A combination of the TUNEL assay and morphology provided the best discrimination between outcome groups.en
dc.language.isoenen
dc.subjectUterine Cervical Canceren
dc.subject.meshAdenocarcinoma-
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAged, 80 and over-
dc.subject.meshApoptosis-
dc.subject.meshCell Division-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshIn Situ Nick-End Labeling-
dc.subject.meshMiddle Aged-
dc.subject.meshMitotic Index-
dc.subject.meshPrognosis-
dc.subject.meshReproducibility of Results-
dc.subject.meshUterine Cervical Neoplasms-
dc.titleA high ratio of apoptosis to proliferation correlates with improved survival after radiotherapy for cervical adenocarcinoma.en
dc.typeArticleen
dc.contributor.departmentCRC Section of Genome Damage and Repair, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom.en
dc.identifier.journalInternational Journal of Radiation Oncology, Biology, Physicsen

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