Tritiated thymidine and bromodeoxyuridine double-labelling studies on growth factors and oral epithelial proliferation in the mouse.

2.50
Hdl Handle:
http://hdl.handle.net/10541/91378
Title:
Tritiated thymidine and bromodeoxyuridine double-labelling studies on growth factors and oral epithelial proliferation in the mouse.
Authors:
Thomson, P J; McGurk, Mark; Potten, Christopher S; Walton, G M; Appleton, D R
Abstract:
Mouse tongue epithelium is characterized by a circadian variation in the number of cells undergoing DNA synthesis. Groups of male BDF1 mice were followed over 48 h and a double-labelling method with tritiated thymidine and bromodeoxyuridine used to determine S-phase labelling indices, together with cell influx to and cell efflux from S, at 4-hourly time points. Control animals exhibited diurnal peaks in labelling index at 03:00 with trough activity 12 h later at 15:00. Cell influx peaked at 23:00 with troughs occurring between 11:00 to 15:00. Peak cell efflux occurred at 07:00 with trough activity at 19:00. Animals injected with epidermal growth factor at 05:00 demonstrated a significant fall in both influx and efflux throughout the 48-h period (P < 0.001), but with preservation of labelling indices, suggesting a slower transit of cells through S-phase, whereas epidermal growth factor injected at 15:00 only produced a significant rise in cell-efflux values. Adrenergic stimulation by intravenous phenylephrine/isoprenaline injection at both 05:00 and 15:00 resulted in a significant rise in cell efflux (P < 0.001), although there was also a rise in labelling index in the 15:00 group (P < 0.001). Animals injected with calmodulin at 05:00 demonstrated a significant reduction in labelling index throughout the 48-h period (P < 0.001), but maintained control values for cell influx and efflux, suggesting faster transit of cells through S. Calmodulin injection at 15:00 produced only a significant reduction in cell influx (P < 0.001). Administration of exogenous growth factors significantly alters the normal rhythmical proliferation of oral epithelial cells in a mouse model. These effects appear to be both growth factor- and time-dependent, and may have both physiological and pathological implications.
Affiliation:
Oral and MaxilloFacial Surgery, The Dental School, Newcastle upon Tyne, UK.
Citation:
Tritiated thymidine and bromodeoxyuridine double-labelling studies on growth factors and oral epithelial proliferation in the mouse. 1999, 44 (9):721-34 Arch. Oral Biol.
Journal:
Archives of Oral Biology
Issue Date:
Sep-1999
URI:
http://hdl.handle.net/10541/91378
DOI:
10.1016/S0003-9969(99)00066-7
PubMed ID:
10471156
Type:
Article
Language:
en
ISSN:
0003-9969
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorThomson, P Jen
dc.contributor.authorMcGurk, Marken
dc.contributor.authorPotten, Christopher Sen
dc.contributor.authorWalton, G Men
dc.contributor.authorAppleton, D Ren
dc.date.accessioned2010-02-08T11:38:03Z-
dc.date.available2010-02-08T11:38:03Z-
dc.date.issued1999-09-
dc.identifier.citationTritiated thymidine and bromodeoxyuridine double-labelling studies on growth factors and oral epithelial proliferation in the mouse. 1999, 44 (9):721-34 Arch. Oral Biol.en
dc.identifier.issn0003-9969-
dc.identifier.pmid10471156-
dc.identifier.doi10.1016/S0003-9969(99)00066-7-
dc.identifier.urihttp://hdl.handle.net/10541/91378-
dc.description.abstractMouse tongue epithelium is characterized by a circadian variation in the number of cells undergoing DNA synthesis. Groups of male BDF1 mice were followed over 48 h and a double-labelling method with tritiated thymidine and bromodeoxyuridine used to determine S-phase labelling indices, together with cell influx to and cell efflux from S, at 4-hourly time points. Control animals exhibited diurnal peaks in labelling index at 03:00 with trough activity 12 h later at 15:00. Cell influx peaked at 23:00 with troughs occurring between 11:00 to 15:00. Peak cell efflux occurred at 07:00 with trough activity at 19:00. Animals injected with epidermal growth factor at 05:00 demonstrated a significant fall in both influx and efflux throughout the 48-h period (P < 0.001), but with preservation of labelling indices, suggesting a slower transit of cells through S-phase, whereas epidermal growth factor injected at 15:00 only produced a significant rise in cell-efflux values. Adrenergic stimulation by intravenous phenylephrine/isoprenaline injection at both 05:00 and 15:00 resulted in a significant rise in cell efflux (P < 0.001), although there was also a rise in labelling index in the 15:00 group (P < 0.001). Animals injected with calmodulin at 05:00 demonstrated a significant reduction in labelling index throughout the 48-h period (P < 0.001), but maintained control values for cell influx and efflux, suggesting faster transit of cells through S. Calmodulin injection at 15:00 produced only a significant reduction in cell influx (P < 0.001). Administration of exogenous growth factors significantly alters the normal rhythmical proliferation of oral epithelial cells in a mouse model. These effects appear to be both growth factor- and time-dependent, and may have both physiological and pathological implications.en
dc.language.isoenen
dc.subject.meshAdrenergic alpha-Agonists-
dc.subject.meshAdrenergic beta-Agonists-
dc.subject.meshAnimals-
dc.subject.meshAntimetabolites-
dc.subject.meshBromodeoxyuridine-
dc.subject.meshCalmodulin-
dc.subject.meshCell Division-
dc.subject.meshCircadian Rhythm-
dc.subject.meshDisease Models, Animal-
dc.subject.meshEpidermal Growth Factor-
dc.subject.meshEpithelial Cells-
dc.subject.meshGrowth Substances-
dc.subject.meshInjections, Intravenous-
dc.subject.meshIsoproterenol-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred Strains-
dc.subject.meshMouth Mucosa-
dc.subject.meshPhenylephrine-
dc.subject.meshRadiopharmaceuticals-
dc.subject.meshS Phase-
dc.subject.meshThymidine-
dc.subject.meshTritium-
dc.titleTritiated thymidine and bromodeoxyuridine double-labelling studies on growth factors and oral epithelial proliferation in the mouse.en
dc.typeArticleen
dc.contributor.departmentOral and MaxilloFacial Surgery, The Dental School, Newcastle upon Tyne, UK.en
dc.identifier.journalArchives of Oral Biologyen
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