2.50
Hdl Handle:
http://hdl.handle.net/10541/91351
Title:
Sequence analysis of heparan sulphate and heparin oligosaccharides.
Authors:
Vivès, Romain R; Pye, David A; Salmivirta, Markku; Hopwood, John J; Lindahl, Ulf; Gallagher, John T
Abstract:
The biological activity of heparan sulphate (HS) and heparin largely depends on internal oligosaccharide sequences that provide specific binding sites for an extensive range of proteins. Identification of such structures is crucial for the complete understanding of glycosaminoglycan (GAG)-protein interactions. We describe here a simple method of sequence analysis relying on the specific tagging of the sugar reducing end by 3H radiolabelling, the combination of chemical scission and specific enzymic digestion to generate intermediate fragments, and the analysis of the generated products by strong-anion-exchange HPLC. We present full sequence data on microgram quantities of four unknown oligosaccharides (three HS-derived hexasaccharides and one heparin-derived octasaccharide) which illustrate the utility and relative simplicity of the technique. The results clearly show that it is also possible to read sequences of inhomogeneous preparations. Application of this technique to biologically active oligosaccharides should accelerate progress in the understanding of HS and heparin structure-function relationships and provide new insights into the primary structure of these polysaccharides.
Affiliation:
CRC section of Drug Development and Imaging, Department of Medical Oncology, Paterson Institute for Cancer Research, Christie Hospital, Manchester M20 4BX, UK.
Citation:
Sequence analysis of heparan sulphate and heparin oligosaccharides. 1999, 339 ( Pt 3):767-73 Biochem. J.
Journal:
The Biochemical Journal
Issue Date:
1-May-1999
URI:
http://hdl.handle.net/10541/91351
PubMed ID:
10215618
Type:
Article
Language:
en
ISSN:
0264-6021
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorVivès, Romain Ren
dc.contributor.authorPye, David Aen
dc.contributor.authorSalmivirta, Markkuen
dc.contributor.authorHopwood, John Jen
dc.contributor.authorLindahl, Ulfen
dc.contributor.authorGallagher, John Ten
dc.date.accessioned2010-02-08T11:22:30Z-
dc.date.available2010-02-08T11:22:30Z-
dc.date.issued1999-05-01-
dc.identifier.citationSequence analysis of heparan sulphate and heparin oligosaccharides. 1999, 339 ( Pt 3):767-73 Biochem. J.en
dc.identifier.issn0264-6021-
dc.identifier.pmid10215618-
dc.identifier.urihttp://hdl.handle.net/10541/91351-
dc.description.abstractThe biological activity of heparan sulphate (HS) and heparin largely depends on internal oligosaccharide sequences that provide specific binding sites for an extensive range of proteins. Identification of such structures is crucial for the complete understanding of glycosaminoglycan (GAG)-protein interactions. We describe here a simple method of sequence analysis relying on the specific tagging of the sugar reducing end by 3H radiolabelling, the combination of chemical scission and specific enzymic digestion to generate intermediate fragments, and the analysis of the generated products by strong-anion-exchange HPLC. We present full sequence data on microgram quantities of four unknown oligosaccharides (three HS-derived hexasaccharides and one heparin-derived octasaccharide) which illustrate the utility and relative simplicity of the technique. The results clearly show that it is also possible to read sequences of inhomogeneous preparations. Application of this technique to biologically active oligosaccharides should accelerate progress in the understanding of HS and heparin structure-function relationships and provide new insights into the primary structure of these polysaccharides.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBinding Sites-
dc.subject.meshCarbohydrate Sequence-
dc.subject.meshChromatography, High Pressure Liquid-
dc.subject.meshDisaccharides-
dc.subject.meshHeparin-
dc.subject.meshHeparitin Sulfate-
dc.subject.meshIduronate Sulfatase-
dc.subject.meshIduronidase-
dc.subject.meshLysosomes-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshMucous Membrane-
dc.subject.meshNitrous Acid-
dc.subject.meshOligosaccharides-
dc.subject.meshProtein Binding-
dc.subject.meshSequence Analysis-
dc.subject.meshStructure-Activity Relationship-
dc.subject.meshSulfatases-
dc.subject.meshSwine-
dc.subject.meshTime Factors-
dc.titleSequence analysis of heparan sulphate and heparin oligosaccharides.en
dc.typeArticleen
dc.contributor.departmentCRC section of Drug Development and Imaging, Department of Medical Oncology, Paterson Institute for Cancer Research, Christie Hospital, Manchester M20 4BX, UK.en
dc.identifier.journalThe Biochemical Journalen
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