Isoflavones inhibit intestinal epithelial cell proliferation and induce apoptosis in vitro.

2.50
Hdl Handle:
http://hdl.handle.net/10541/90794
Title:
Isoflavones inhibit intestinal epithelial cell proliferation and induce apoptosis in vitro.
Authors:
Booth, Catherine; Hargreaves, Danielle F; Hadfield, John A; McGown, Alan T; Potten, Christopher S
Abstract:
There have been many reports that high soya-based diets reduce the risk of certain types of cancer. This effect may be due to the presence of high levels of isoflavones derived from the soya bean, particularly genistein which has been shown to be a protein tyrosine kinase (PTK) inhibitor and have both oestrogenic and anti-oestrogenic properties. We have examined the effect of genistein and a number of novel synthetic analogues on both normal (IEC6, IEC18) and transformed (SW620, HT29) intestinal epithelial cell lines. Responses were compared to those elicited by oestradiol, the anti-oestrogen tamoxifen, and the tyrosine kinase inhibitor tyrphostin. Genistein and tamoxifen were potent inhibitors of cell proliferation. Of seven novel isoflavones tested, none were more potent inhibitors than genistein, and all displayed similar relative activities across the different cell lines. In addition to inhibiting cell proliferation, cell death via apoptosis was observed when the cells were exposed to the isoflavones and all but one exhibited PTK inhibitory activity. These data suggest that by reducing proliferation and inducing apoptosis, possibly due in part to PTK inhibition, isoflavones may have a role in protecting normal intestinal epithelium from tumour development (reducing the risk) and may reduce colonic tumour growth.
Affiliation:
Epithelial Biology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Citation:
Isoflavones inhibit intestinal epithelial cell proliferation and induce apoptosis in vitro. 1999, 80 (10):1550-7 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
Jul-1999
URI:
http://hdl.handle.net/10541/90794
DOI:
10.1038/sj.bjc.6690559
PubMed ID:
10408396
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBooth, Catherineen
dc.contributor.authorHargreaves, Danielle Fen
dc.contributor.authorHadfield, John Aen
dc.contributor.authorMcGown, Alan Ten
dc.contributor.authorPotten, Christopher Sen
dc.date.accessioned2010-01-28T11:35:41Z-
dc.date.available2010-01-28T11:35:41Z-
dc.date.issued1999-07-
dc.identifier.citationIsoflavones inhibit intestinal epithelial cell proliferation and induce apoptosis in vitro. 1999, 80 (10):1550-7 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid10408396-
dc.identifier.doi10.1038/sj.bjc.6690559-
dc.identifier.urihttp://hdl.handle.net/10541/90794-
dc.description.abstractThere have been many reports that high soya-based diets reduce the risk of certain types of cancer. This effect may be due to the presence of high levels of isoflavones derived from the soya bean, particularly genistein which has been shown to be a protein tyrosine kinase (PTK) inhibitor and have both oestrogenic and anti-oestrogenic properties. We have examined the effect of genistein and a number of novel synthetic analogues on both normal (IEC6, IEC18) and transformed (SW620, HT29) intestinal epithelial cell lines. Responses were compared to those elicited by oestradiol, the anti-oestrogen tamoxifen, and the tyrosine kinase inhibitor tyrphostin. Genistein and tamoxifen were potent inhibitors of cell proliferation. Of seven novel isoflavones tested, none were more potent inhibitors than genistein, and all displayed similar relative activities across the different cell lines. In addition to inhibiting cell proliferation, cell death via apoptosis was observed when the cells were exposed to the isoflavones and all but one exhibited PTK inhibitory activity. These data suggest that by reducing proliferation and inducing apoptosis, possibly due in part to PTK inhibition, isoflavones may have a role in protecting normal intestinal epithelium from tumour development (reducing the risk) and may reduce colonic tumour growth.en
dc.language.isoenen
dc.subjectColonic Canceren
dc.subjectLeukaemiaen
dc.subjectCultured Tumour Cellsen
dc.subject.meshAnimals-
dc.subject.meshApoptosis-
dc.subject.meshCell Division-
dc.subject.meshColonic Neoplasms-
dc.subject.meshEnzyme Inhibitors-
dc.subject.meshHumans-
dc.subject.meshIntestinal Mucosa-
dc.subject.meshIsoflavones-
dc.subject.meshLeukemia P388-
dc.subject.meshProtein-Tyrosine Kinases-
dc.subject.meshRats-
dc.subject.meshTamoxifen-
dc.subject.meshTumor Cells, Cultured-
dc.subject.meshTyrphostins-
dc.titleIsoflavones inhibit intestinal epithelial cell proliferation and induce apoptosis in vitro.en
dc.typeArticleen
dc.contributor.departmentEpithelial Biology Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren

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