In vivo administration of genistein has no effect on small intestinal epithelial proliferation and apoptosis, but a modest effect on clonogen survival.

2.50
Hdl Handle:
http://hdl.handle.net/10541/90789
Title:
In vivo administration of genistein has no effect on small intestinal epithelial proliferation and apoptosis, but a modest effect on clonogen survival.
Authors:
Booth, Catherine; Hargreaves, Danielle F; O'Shea, Julie A; Potten, Christopher S
Abstract:
The soya metabolite genistein possesses anti-proliferative, pro-apoptotic activities in intestinal epithelial cells in vitro and may reduce epithelial cancer incidence. This could involve cell cycle arrest/apoptosis in the proliferative or clonogenic cells. We investigated the effects of genistein on the small intestinal epithelium in vivo. No effect on the number or distribution of proliferative cells in the crypts was detected. Similarly, no change in spontaneous apoptotic cell incidence or the characteristic stem cell apoptotic response following low dose irradiation was observed. Genistein afforded a modest decrease in clonogen radiosensitivity. Hence, using a range of dosing protocols, sub-cutaneous administration of genistein for periods of up to 1 week did not alter intestinal epithelial homeostasis.
Affiliation:
CRC Epithelial Biology Group, Cell and Tissue Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK. cbooth@picr.man.ac.uk
Citation:
In vivo administration of genistein has no effect on small intestinal epithelial proliferation and apoptosis, but a modest effect on clonogen survival. 1999, 144 (2):169-75 Cancer Lett.
Journal:
Cancer Letters
Issue Date:
1-Oct-1999
URI:
http://hdl.handle.net/10541/90789
DOI:
10.1016/S0304-3835(99)00220-7
PubMed ID:
10529017
Type:
Article
Language:
en
ISSN:
0304-3835
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBooth, Catherineen
dc.contributor.authorHargreaves, Danielle Fen
dc.contributor.authorO'Shea, Julie Aen
dc.contributor.authorPotten, Christopher Sen
dc.date.accessioned2010-01-28T10:58:38Z-
dc.date.available2010-01-28T10:58:38Z-
dc.date.issued1999-10-01-
dc.identifier.citationIn vivo administration of genistein has no effect on small intestinal epithelial proliferation and apoptosis, but a modest effect on clonogen survival. 1999, 144 (2):169-75 Cancer Lett.en
dc.identifier.issn0304-3835-
dc.identifier.pmid10529017-
dc.identifier.doi10.1016/S0304-3835(99)00220-7-
dc.identifier.urihttp://hdl.handle.net/10541/90789-
dc.description.abstractThe soya metabolite genistein possesses anti-proliferative, pro-apoptotic activities in intestinal epithelial cells in vitro and may reduce epithelial cancer incidence. This could involve cell cycle arrest/apoptosis in the proliferative or clonogenic cells. We investigated the effects of genistein on the small intestinal epithelium in vivo. No effect on the number or distribution of proliferative cells in the crypts was detected. Similarly, no change in spontaneous apoptotic cell incidence or the characteristic stem cell apoptotic response following low dose irradiation was observed. Genistein afforded a modest decrease in clonogen radiosensitivity. Hence, using a range of dosing protocols, sub-cutaneous administration of genistein for periods of up to 1 week did not alter intestinal epithelial homeostasis.en
dc.language.isoenen
dc.subjectOestrogen Receptor Modulatorsen
dc.subject.meshAnimals-
dc.subject.meshAnticarcinogenic Agents-
dc.subject.meshApoptosis-
dc.subject.meshCell Division-
dc.subject.meshCell Survival-
dc.subject.meshClone Cells-
dc.subject.meshEpithelial Cells-
dc.subject.meshEstradiol-
dc.subject.meshEstrogen Receptor Modulators-
dc.subject.meshGenistein-
dc.subject.meshIntestine, Small-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshStem Cells-
dc.subject.meshTamoxifen-
dc.subject.meshTyrphostins-
dc.titleIn vivo administration of genistein has no effect on small intestinal epithelial proliferation and apoptosis, but a modest effect on clonogen survival.en
dc.typeArticleen
dc.contributor.departmentCRC Epithelial Biology Group, Cell and Tissue Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK. cbooth@picr.man.ac.uken
dc.identifier.journalCancer Lettersen

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