2.50
Hdl Handle:
http://hdl.handle.net/10541/90785
Title:
Highly sensitive sequencing of the sulfated domains of heparan sulfate.
Authors:
Merry, Catherine L R; Lyon, Malcolm; Deakin, Jon A; Hopwood, John J; Gallagher, John T
Abstract:
The heparan sulfates (HS) are hypervariable linear polysaccharides that act as membrane co-receptors for growth factors, chemokines, and extracellular matrix proteins. In most instances, the molecular basis of protein recognition by HS is poorly understood. We have sequenced 75% of the sulfated domains (S-domains) of fibroblast HS, including all of the major ones. This analysis revealed tight coupling of N- and 2-O-sulfation and a low frequency but precise positioning of 6-O-sulfates, which are required functional groups for HS-mediated activation of the fibroblast growth factors. S-domain sequencing was conducted using a novel and highly sensitive method based on a new way of reading the sequence from high performance liquid chromatography separation profiles of metabolically labeled HS-saccharides following specific chemical and enzymatic scission. The implications of the patterns seen in the sulfated domains for better understanding of the synthesis and function of HS are discussed.
Affiliation:
Cancer Research Campaign and University of Manchester Department of Medical Oncology, Christie Hospital NHS Trust, Manchester, M20 4BX, United Kingdom.
Citation:
Highly sensitive sequencing of the sulfated domains of heparan sulfate. 1999, 274 (26):18455-62 J. Biol. Chem.
Journal:
Journal of Biological Chemistry
Issue Date:
25-Jun-1999
URI:
http://hdl.handle.net/10541/90785
DOI:
10.1074/jbc.274.26.18455
PubMed ID:
10373453
Type:
Article
Language:
en
ISSN:
0021-9258
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMerry, Catherine L Ren
dc.contributor.authorLyon, Malcolmen
dc.contributor.authorDeakin, Jon Aen
dc.contributor.authorHopwood, John Jen
dc.contributor.authorGallagher, John Ten
dc.date.accessioned2010-01-28T10:24:29Z-
dc.date.available2010-01-28T10:24:29Z-
dc.date.issued1999-06-25-
dc.identifier.citationHighly sensitive sequencing of the sulfated domains of heparan sulfate. 1999, 274 (26):18455-62 J. Biol. Chem.en
dc.identifier.issn0021-9258-
dc.identifier.pmid10373453-
dc.identifier.doi10.1074/jbc.274.26.18455-
dc.identifier.urihttp://hdl.handle.net/10541/90785-
dc.description.abstractThe heparan sulfates (HS) are hypervariable linear polysaccharides that act as membrane co-receptors for growth factors, chemokines, and extracellular matrix proteins. In most instances, the molecular basis of protein recognition by HS is poorly understood. We have sequenced 75% of the sulfated domains (S-domains) of fibroblast HS, including all of the major ones. This analysis revealed tight coupling of N- and 2-O-sulfation and a low frequency but precise positioning of 6-O-sulfates, which are required functional groups for HS-mediated activation of the fibroblast growth factors. S-domain sequencing was conducted using a novel and highly sensitive method based on a new way of reading the sequence from high performance liquid chromatography separation profiles of metabolically labeled HS-saccharides following specific chemical and enzymatic scission. The implications of the patterns seen in the sulfated domains for better understanding of the synthesis and function of HS are discussed.en
dc.language.isoenen
dc.subject.mesh3T3 Cells-
dc.subject.meshAnimals-
dc.subject.meshCarbohydrate Sequence-
dc.subject.meshChromatography, Gel-
dc.subject.meshChromatography, High Pressure Liquid-
dc.subject.meshHeparitin Sulfate-
dc.subject.meshMice-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshSulfates-
dc.titleHighly sensitive sequencing of the sulfated domains of heparan sulfate.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign and University of Manchester Department of Medical Oncology, Christie Hospital NHS Trust, Manchester, M20 4BX, United Kingdom.en
dc.identifier.journalJournal of Biological Chemistryen

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