2.50
Hdl Handle:
http://hdl.handle.net/10541/90780
Title:
Expression of proteoglycan core proteins in human bone marrow stroma.
Authors:
Schofield, Karen P; Gallagher, John T; David, Guido
Abstract:
Heparan sulphate proteoglycans (HSPGs) present on the surface of bone marrow stromal cells and in the extracellular matrix (ECM) have important roles in the control of adhesion and growth of haemopoietic stem and progenitor cells. The two main groups of proteoglycans which contain heparan sulphate chains are members of the syndecan and glypican families. In this study we have identified the main surface membrane and matrix-associated HSPGs present in normal human bone marrow stroma formed in long-term culture. Proteoglycans were extracted from the adherent stromal layers and treated with heparitinase and chondroitinase ABC. The core proteins were detected by Western blotting using antibodies directed against syndecans-1-4, glypican-1 and the ECM HSPG, perlecan. Stromal cell expression at the RNA level was detected by Northern blotting and by reverse transcription PCR. Glypican-1, syndecan-3 and syndecan-4 were the major cell-membrane HSPG species and perlecan was the major ECM proteoglycan. There was no evidence for expression of syndecan-1 protein. Syndecan-3 was expressed mainly as a variant or processed 50-55 kDa core protein and in lower amounts as the characteristic 125 kDa core protein. These results suggest that syndecan-3, syndecan-4 and glypican-1 present on the surface of marrow stromal cells, together with perlecan in the ECM, may be responsible for creating the correct stromal 'niche' for the maintenance and development of haemopoietic stem and progenitor cells. The detection of a variant form of syndecan-3 as a major stromal HSPG suggests a specific role for this syndecan in haemopoiesis.
Affiliation:
Department of Medical Oncology, Paterson Institute for Cancer Research, Wilmslow Road, Manchester M20 4BX, UK. KSchofield@picr.man.ac.uk
Citation:
Expression of proteoglycan core proteins in human bone marrow stroma. 1999, 343 Pt 3:663-8 Biochem. J.
Journal:
Biochemical Journal
Issue Date:
1-Nov-1999
URI:
http://hdl.handle.net/10541/90780
PubMed ID:
10527946
Type:
Article
Language:
en
ISSN:
0264-6021
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorSchofield, Karen Pen
dc.contributor.authorGallagher, John Ten
dc.contributor.authorDavid, Guidoen
dc.date.accessioned2010-01-28T10:09:56Z-
dc.date.available2010-01-28T10:09:56Z-
dc.date.issued1999-11-01-
dc.identifier.citationExpression of proteoglycan core proteins in human bone marrow stroma. 1999, 343 Pt 3:663-8 Biochem. J.en
dc.identifier.issn0264-6021-
dc.identifier.pmid10527946-
dc.identifier.urihttp://hdl.handle.net/10541/90780-
dc.description.abstractHeparan sulphate proteoglycans (HSPGs) present on the surface of bone marrow stromal cells and in the extracellular matrix (ECM) have important roles in the control of adhesion and growth of haemopoietic stem and progenitor cells. The two main groups of proteoglycans which contain heparan sulphate chains are members of the syndecan and glypican families. In this study we have identified the main surface membrane and matrix-associated HSPGs present in normal human bone marrow stroma formed in long-term culture. Proteoglycans were extracted from the adherent stromal layers and treated with heparitinase and chondroitinase ABC. The core proteins were detected by Western blotting using antibodies directed against syndecans-1-4, glypican-1 and the ECM HSPG, perlecan. Stromal cell expression at the RNA level was detected by Northern blotting and by reverse transcription PCR. Glypican-1, syndecan-3 and syndecan-4 were the major cell-membrane HSPG species and perlecan was the major ECM proteoglycan. There was no evidence for expression of syndecan-1 protein. Syndecan-3 was expressed mainly as a variant or processed 50-55 kDa core protein and in lower amounts as the characteristic 125 kDa core protein. These results suggest that syndecan-3, syndecan-4 and glypican-1 present on the surface of marrow stromal cells, together with perlecan in the ECM, may be responsible for creating the correct stromal 'niche' for the maintenance and development of haemopoietic stem and progenitor cells. The detection of a variant form of syndecan-3 as a major stromal HSPG suggests a specific role for this syndecan in haemopoiesis.en
dc.language.isoenen
dc.subject.meshAggrecans-
dc.subject.meshBone Marrow Cells-
dc.subject.meshCells, Cultured-
dc.subject.meshExtracellular Matrix Proteins-
dc.subject.meshGlycoproteins-
dc.subject.meshHumans-
dc.subject.meshLectins, C-Type-
dc.subject.meshMembrane Glycoproteins-
dc.subject.meshProteoglycans-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction-
dc.subject.meshStromal Cells-
dc.subject.meshSyndecan-1-
dc.subject.meshSyndecans-
dc.subject.meshTranscription, Genetic-
dc.titleExpression of proteoglycan core proteins in human bone marrow stroma.en
dc.typeArticleen
dc.contributor.departmentDepartment of Medical Oncology, Paterson Institute for Cancer Research, Wilmslow Road, Manchester M20 4BX, UK. KSchofield@picr.man.ac.uken
dc.identifier.journalBiochemical Journalen

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