2.50
Hdl Handle:
http://hdl.handle.net/10541/90779
Title:
Heparin is a unique marker of progenitors in the glial cell lineage.
Authors:
Stringer, Sally E; Mayer-Proschel, Margot; Kalyani, Anjali; Rao, Mahendra; Gallagher, John T
Abstract:
The oligodendrocyte-type-2 astrocyte progenitor cells (precursors of oligodendrocytes and type-2 astrocytes) are an excellent system in which to study differentiation as they can be manipulated in vitro. Maintenance of oligodendrocyte-type-2 astrocyte progenitor cells requires basic fibroblast growth factor, a growth factor whose action normally depends on a heparan sulfate coreceptor. Biochemical analysis revealed a most surprising result: that the oligodendrocyte-type-2 astrocyte progenitors did not synthesize heparan sulfate, the near ubiquitous N-sulfated cell surface polysaccharide, but the chemically related heparin in a form that was almost completely N- and O-sulfated. The heparin was detected in the pericellular fraction of the cells and the culture medium. In contrast the differentiated glial subpopulations (oligodendrocytes and type-2 astrocytes) synthesized typical heparan sulfate but with distinctive fine structural features for each cell type. Thus heparin is a unique differentiation marker in the glial lineage. Previously heparin has been found only in a subset of mature mast cells called the connective tissue mast cells. Its presence within the developing nervous system on a precise population of progenitors may confer specific and essential recognition properties on those cells in relation to binding soluble growth and/or differentiation factors and the extracellular matrix.
Affiliation:
Cancer Research Campaign, Paterson Institute of Cancer Research, Christie Hospital, Wilmslow Road, Manchester M20 4BX, United Kingdom. sstringer@picr.man.ac.uk
Citation:
Heparin is a unique marker of progenitors in the glial cell lineage. 1999, 274 (36):25455-60 J. Biol. Chem.
Journal:
The Journal of Biological Chemistry
Issue Date:
3-Sep-1999
URI:
http://hdl.handle.net/10541/90779
DOI:
10.1074/jbc.274.36.25455
PubMed ID:
10464276
Type:
Article
Language:
en
ISSN:
0021-9258
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorStringer, Sally Een
dc.contributor.authorMayer-Proschel, Margoten
dc.contributor.authorKalyani, Anjalien
dc.contributor.authorRao, Mahendraen
dc.contributor.authorGallagher, John Ten
dc.date.accessioned2010-01-28T10:04:29Z-
dc.date.available2010-01-28T10:04:29Z-
dc.date.issued1999-09-03-
dc.identifier.citationHeparin is a unique marker of progenitors in the glial cell lineage. 1999, 274 (36):25455-60 J. Biol. Chem.en
dc.identifier.issn0021-9258-
dc.identifier.pmid10464276-
dc.identifier.doi10.1074/jbc.274.36.25455-
dc.identifier.urihttp://hdl.handle.net/10541/90779-
dc.description.abstractThe oligodendrocyte-type-2 astrocyte progenitor cells (precursors of oligodendrocytes and type-2 astrocytes) are an excellent system in which to study differentiation as they can be manipulated in vitro. Maintenance of oligodendrocyte-type-2 astrocyte progenitor cells requires basic fibroblast growth factor, a growth factor whose action normally depends on a heparan sulfate coreceptor. Biochemical analysis revealed a most surprising result: that the oligodendrocyte-type-2 astrocyte progenitors did not synthesize heparan sulfate, the near ubiquitous N-sulfated cell surface polysaccharide, but the chemically related heparin in a form that was almost completely N- and O-sulfated. The heparin was detected in the pericellular fraction of the cells and the culture medium. In contrast the differentiated glial subpopulations (oligodendrocytes and type-2 astrocytes) synthesized typical heparan sulfate but with distinctive fine structural features for each cell type. Thus heparin is a unique differentiation marker in the glial lineage. Previously heparin has been found only in a subset of mature mast cells called the connective tissue mast cells. Its presence within the developing nervous system on a precise population of progenitors may confer specific and essential recognition properties on those cells in relation to binding soluble growth and/or differentiation factors and the extracellular matrix.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBiological Markers-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Lineage-
dc.subject.meshCells, Cultured-
dc.subject.meshHeparin-
dc.subject.meshOligodendroglia-
dc.subject.meshRats-
dc.subject.meshStem Cells-
dc.titleHeparin is a unique marker of progenitors in the glial cell lineage.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign, Paterson Institute of Cancer Research, Christie Hospital, Wilmslow Road, Manchester M20 4BX, United Kingdom. sstringer@picr.man.ac.uken
dc.identifier.journalThe Journal of Biological Chemistryen
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