Photoprotection by topical DNA repair enzymes: molecular correlates of clinical studies.

2.50
Hdl Handle:
http://hdl.handle.net/10541/90776
Title:
Photoprotection by topical DNA repair enzymes: molecular correlates of clinical studies.
Authors:
Yarosh, Daniel; O'Connor, Adrienne; Alas, Lori; Potten, Christopher S; Wolf, Peter
Abstract:
A new approach to photoprotection is to repair DNA damage after UV exposure. This can be accomplished by delivery of a DNA repair enzyme with specificity to UV-induced cyclobutane pyrimidine dimers into skin by means of specially engineered liposomes. Treatment of DNA-repair-deficient xeroderma pigmentosum patients or skin cancer patients with T4N5 liposome lotion containing such DNA repair liposomes increases the removal of DNA damage in the first few hours after treatment. In these studies, a DNA repair effect was observed in some patients treated with heat-inactivated enzyme. Unexpectedly, it was discovered that the heat-inactivated T4 endonuclease V enzyme refolds and recovers enzymatic activity. These studies demonstrate that measurements of molecular changes induced by biological drugs are useful adjuvants to clinical studies.
Affiliation:
AGI Dermatics, Freeport, NY 11420, USA. agiderm@aol.com
Citation:
Photoprotection by topical DNA repair enzymes: molecular correlates of clinical studies. 1999, 69 (2):136-40 Photochem. Photobiol.
Journal:
Photochemistry and Photobiology
Issue Date:
Feb-1999
URI:
http://hdl.handle.net/10541/90776
DOI:
10.1111/j.1751-1097.1999.tb03265.x
PubMed ID:
10048308
Type:
Article
Language:
en
ISSN:
0031-8655
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorYarosh, Danielen
dc.contributor.authorO'Connor, Adrienneen
dc.contributor.authorAlas, Lorien
dc.contributor.authorPotten, Christopher Sen
dc.contributor.authorWolf, Peteren
dc.date.accessioned2010-01-28T09:59:05Z-
dc.date.available2010-01-28T09:59:05Z-
dc.date.issued1999-02-
dc.identifier.citationPhotoprotection by topical DNA repair enzymes: molecular correlates of clinical studies. 1999, 69 (2):136-40 Photochem. Photobiol.en
dc.identifier.issn0031-8655-
dc.identifier.pmid10048308-
dc.identifier.doi10.1111/j.1751-1097.1999.tb03265.x-
dc.identifier.urihttp://hdl.handle.net/10541/90776-
dc.description.abstractA new approach to photoprotection is to repair DNA damage after UV exposure. This can be accomplished by delivery of a DNA repair enzyme with specificity to UV-induced cyclobutane pyrimidine dimers into skin by means of specially engineered liposomes. Treatment of DNA-repair-deficient xeroderma pigmentosum patients or skin cancer patients with T4N5 liposome lotion containing such DNA repair liposomes increases the removal of DNA damage in the first few hours after treatment. In these studies, a DNA repair effect was observed in some patients treated with heat-inactivated enzyme. Unexpectedly, it was discovered that the heat-inactivated T4 endonuclease V enzyme refolds and recovers enzymatic activity. These studies demonstrate that measurements of molecular changes induced by biological drugs are useful adjuvants to clinical studies.en
dc.language.isoenen
dc.subjectSkin Canceren
dc.subject.meshAdministration, Topical-
dc.subject.meshAnimals-
dc.subject.meshDNA Repair-
dc.subject.meshDeoxyribonuclease (Pyrimidine Dimer)-
dc.subject.meshDrug Carriers-
dc.subject.meshEndodeoxyribonucleases-
dc.subject.meshLiposomes-
dc.subject.meshOintments-
dc.subject.meshProtein Folding-
dc.subject.meshSkin-
dc.subject.meshSkin Neoplasms-
dc.subject.meshUltraviolet Rays-
dc.subject.meshViral Proteins-
dc.subject.meshXeroderma Pigmentosum-
dc.titlePhotoprotection by topical DNA repair enzymes: molecular correlates of clinical studies.en
dc.typeArticleen
dc.contributor.departmentAGI Dermatics, Freeport, NY 11420, USA. agiderm@aol.comen
dc.identifier.journalPhotochemistry and Photobiologyen

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