Mapping dynamic epithelial cell proliferative activity within the oral cavity of man: a new insight into carcinogenesis?

2.50
Hdl Handle:
http://hdl.handle.net/10541/90765
Title:
Mapping dynamic epithelial cell proliferative activity within the oral cavity of man: a new insight into carcinogenesis?
Authors:
Thomson, P J; Potten, Christopher S; Appleton, D R
Abstract:
Our aim was to characterize epithelial cell proliferative activity within the oral cavity and to find out if there were differences between sites with high and low incidence of cancer. A total of 105 samples of clinically normal mucosa were harvested from various intra-oral sites. Excised specimens were incubated in vitro with tritiated thymidine and bromodeoxyuridine to 'double label' cells undergoing DNA synthesis, and enable calculation of the duration of S phase and estimation of variables of cell flux to and from S. Mean labelling indices (percentage of cells within the S phase of the cell cycle) were highest in the floor of mouth (12.3%) and ventral tongue (10.1%), while activity was lowest in the dorsum of tongue (4.3%) and the palate (7.2%), P<0.001. In general, both cell influx and the duration of S increased proportionally to the labelling index. Sites with a high incidence of cancer were characterized by high labelling indices, increased cell influx and a prolonged S phase.
Affiliation:
Department of Oral & Maxillofacial Surgery, The Dental School, University of Newcastle upon Tyne, UK.
Citation:
Mapping dynamic epithelial cell proliferative activity within the oral cavity of man: a new insight into carcinogenesis? 1999, 37 (5):377-83 Br J Oral Maxillofac Surg
Journal:
The British Journal of Oral & Maxillofacial Surgery
Issue Date:
Oct-1999
URI:
http://hdl.handle.net/10541/90765
DOI:
10.1054/bjom.1999.0130
PubMed ID:
10577752
Type:
Article
Language:
en
ISSN:
0266-4356
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorThomson, P Jen
dc.contributor.authorPotten, Christopher Sen
dc.contributor.authorAppleton, D Ren
dc.date.accessioned2010-01-28T12:08:08Z-
dc.date.available2010-01-28T12:08:08Z-
dc.date.issued1999-10-
dc.identifier.citationMapping dynamic epithelial cell proliferative activity within the oral cavity of man: a new insight into carcinogenesis? 1999, 37 (5):377-83 Br J Oral Maxillofac Surgen
dc.identifier.issn0266-4356-
dc.identifier.pmid10577752-
dc.identifier.doi10.1054/bjom.1999.0130-
dc.identifier.urihttp://hdl.handle.net/10541/90765-
dc.description.abstractOur aim was to characterize epithelial cell proliferative activity within the oral cavity and to find out if there were differences between sites with high and low incidence of cancer. A total of 105 samples of clinically normal mucosa were harvested from various intra-oral sites. Excised specimens were incubated in vitro with tritiated thymidine and bromodeoxyuridine to 'double label' cells undergoing DNA synthesis, and enable calculation of the duration of S phase and estimation of variables of cell flux to and from S. Mean labelling indices (percentage of cells within the S phase of the cell cycle) were highest in the floor of mouth (12.3%) and ventral tongue (10.1%), while activity was lowest in the dorsum of tongue (4.3%) and the palate (7.2%), P<0.001. In general, both cell influx and the duration of S increased proportionally to the labelling index. Sites with a high incidence of cancer were characterized by high labelling indices, increased cell influx and a prolonged S phase.en
dc.language.isoenen
dc.subjectMouth Canceren
dc.subject.meshAdult-
dc.subject.meshAnalysis of Variance-
dc.subject.meshCell Division-
dc.subject.meshCytological Techniques-
dc.subject.meshEpithelial Cells-
dc.subject.meshHumans-
dc.subject.meshMouth Mucosa-
dc.subject.meshMouth Neoplasms-
dc.subject.meshReference Values-
dc.titleMapping dynamic epithelial cell proliferative activity within the oral cavity of man: a new insight into carcinogenesis?en
dc.typeArticleen
dc.contributor.departmentDepartment of Oral & Maxillofacial Surgery, The Dental School, University of Newcastle upon Tyne, UK.en
dc.identifier.journalThe British Journal of Oral & Maxillofacial Surgeryen
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