Potential of radiation-induced chromosome aberrations to predict radiosensitivity in human tumour cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/90176
Title:
Potential of radiation-induced chromosome aberrations to predict radiosensitivity in human tumour cells.
Authors:
Coco Martin, J M; Mooren, E; Ottenheim, C; Burrill, Wayne; Nunez, M I; Sprong, D; Bartelink, Harry; Begg, Adrian C
Abstract:
PURPOSE: To validate whether the number of aberrations could be used as a measure of the radiosensitivity of human tumour cells. If so, this would potentially provide a more rapid method than the colony assay to predict radiocurability in human tumour biopsy material. MATERIALS AND METHODS: A panel of 13 human tumour cell lines was investigated, covering a wide range of radiosensitivities. Fluorescence in situ hybridization (FISH) employing whole chromosome probes was used to detect aberrations. RESULTS: A dose-dependent increase in radiation-induced chromosome aberrations was observed in all cell lines. A good correlation (r=0.90) was found between cell survival and total chromosome aberrations in 12 of the 13 cell lines (92%), with one exception. A poorer correlation was observed between cell survival and stable- (r=0.85) and unstable-type aberrations (r=0.81). Survival-aberration correlations for individual radiation doses were worse, although statistically significant. The exceptional cell line showed significantly more aberrations for a given level of cell kill than expected based on data for the other lines. CONCLUSION: This study indicates that radiation-induced chromosome aberrations can be used as a potential predictor of intrinsic radiosensitivity for the majority of human tumours when more than one dose level is tested. This could aid the design of radiotherapy schedules for each individual patient, or in the decision of whether to use an alternative therapy.
Affiliation:
Department of Radiotherapy, The Netherlands Cancer Institute, Amsterdam. jose.coco_martin@gist-brocades.infonet.com
Citation:
Potential of radiation-induced chromosome aberrations to predict radiosensitivity in human tumour cells. 1999, 75 (9):1161-8 Int. J. Radiat. Biol.
Journal:
International Journal of Radiation Biology
Issue Date:
Sep-1999
URI:
http://hdl.handle.net/10541/90176
PubMed ID:
10528924
Type:
Article
Language:
en
ISSN:
0955-3002
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorCoco Martin, J Men
dc.contributor.authorMooren, Een
dc.contributor.authorOttenheim, Cen
dc.contributor.authorBurrill, Wayneen
dc.contributor.authorNunez, M Ien
dc.contributor.authorSprong, Den
dc.contributor.authorBartelink, Harryen
dc.contributor.authorBegg, Adrian Cen
dc.date.accessioned2010-01-20T16:21:15Z-
dc.date.available2010-01-20T16:21:15Z-
dc.date.issued1999-09-
dc.identifier.citationPotential of radiation-induced chromosome aberrations to predict radiosensitivity in human tumour cells. 1999, 75 (9):1161-8 Int. J. Radiat. Biol.en
dc.identifier.issn0955-3002-
dc.identifier.pmid10528924-
dc.identifier.urihttp://hdl.handle.net/10541/90176-
dc.description.abstractPURPOSE: To validate whether the number of aberrations could be used as a measure of the radiosensitivity of human tumour cells. If so, this would potentially provide a more rapid method than the colony assay to predict radiocurability in human tumour biopsy material. MATERIALS AND METHODS: A panel of 13 human tumour cell lines was investigated, covering a wide range of radiosensitivities. Fluorescence in situ hybridization (FISH) employing whole chromosome probes was used to detect aberrations. RESULTS: A dose-dependent increase in radiation-induced chromosome aberrations was observed in all cell lines. A good correlation (r=0.90) was found between cell survival and total chromosome aberrations in 12 of the 13 cell lines (92%), with one exception. A poorer correlation was observed between cell survival and stable- (r=0.85) and unstable-type aberrations (r=0.81). Survival-aberration correlations for individual radiation doses were worse, although statistically significant. The exceptional cell line showed significantly more aberrations for a given level of cell kill than expected based on data for the other lines. CONCLUSION: This study indicates that radiation-induced chromosome aberrations can be used as a potential predictor of intrinsic radiosensitivity for the majority of human tumours when more than one dose level is tested. This could aid the design of radiotherapy schedules for each individual patient, or in the decision of whether to use an alternative therapy.en
dc.language.isoenen
dc.subjectCanceren
dc.subjectCultured Cancer Cellsen
dc.subject.meshCell Survival-
dc.subject.meshChromosome Aberrations-
dc.subject.meshDose-Response Relationship, Radiation-
dc.subject.meshHumans-
dc.subject.meshNeoplasms-
dc.subject.meshRadiation Tolerance-
dc.subject.meshTumor Cells, Cultured-
dc.titlePotential of radiation-induced chromosome aberrations to predict radiosensitivity in human tumour cells.en
dc.typeArticleen
dc.contributor.departmentDepartment of Radiotherapy, The Netherlands Cancer Institute, Amsterdam. jose.coco_martin@gist-brocades.infonet.comen
dc.identifier.journalInternational Journal of Radiation Biologyen

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