Recombinant vaccinia viruses protect against Clostridium perfringens alpha-toxin.

2.50
Hdl Handle:
http://hdl.handle.net/10541/90173
Title:
Recombinant vaccinia viruses protect against Clostridium perfringens alpha-toxin.
Authors:
Bennett, Alice M; Lescott, Thelma; Phillpotts, Robert J; Mackett, Mike; Titball, Richard W
Abstract:
Recombinant vaccinia viruses that expressed the nontoxic C-domain of Clostridium perfringens alpha-toxin were constructed. The J2R (thymidine kinase [TK] gene) and B13R (serpin 2 [SPI-2] gene) loci were used as insertion sites for the clostridial DNA, and expression of the foreign protein was measured in each case. A double recombinant that encoded the alpha-toxin truncate at the B13R locus and the protective antigen of Bacillus anthracis at the J2R locus was also constructed. Although differences in expression of the alpha-toxin C-domain were recorded, all of the vaccinia recombinants protected mice against a lethal challenge with alpha-toxin demonstrating that a recombinant vaccinia virus can be used to provide protection against a toxin challenge that is known to be solely antibody mediated.
Affiliation:
Defence Evaluation and Research Agency, Salisbury, Wiltshire, United Kingdom.
Citation:
Recombinant vaccinia viruses protect against Clostridium perfringens alpha-toxin. 1999, 12 (2):97-105 Viral Immunol.
Journal:
Viral Immunology
Issue Date:
1999
URI:
http://hdl.handle.net/10541/90173
PubMed ID:
10413356
Type:
Article
Language:
en
ISSN:
0882-8245
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBennett, Alice Men
dc.contributor.authorLescott, Thelmaen
dc.contributor.authorPhillpotts, Robert Jen
dc.contributor.authorMackett, Mikeen
dc.contributor.authorTitball, Richard Wen
dc.date.accessioned2010-01-20T16:00:52Z-
dc.date.available2010-01-20T16:00:52Z-
dc.date.issued1999-
dc.identifier.citationRecombinant vaccinia viruses protect against Clostridium perfringens alpha-toxin. 1999, 12 (2):97-105 Viral Immunol.en
dc.identifier.issn0882-8245-
dc.identifier.pmid10413356-
dc.identifier.urihttp://hdl.handle.net/10541/90173-
dc.description.abstractRecombinant vaccinia viruses that expressed the nontoxic C-domain of Clostridium perfringens alpha-toxin were constructed. The J2R (thymidine kinase [TK] gene) and B13R (serpin 2 [SPI-2] gene) loci were used as insertion sites for the clostridial DNA, and expression of the foreign protein was measured in each case. A double recombinant that encoded the alpha-toxin truncate at the B13R locus and the protective antigen of Bacillus anthracis at the J2R locus was also constructed. Although differences in expression of the alpha-toxin C-domain were recorded, all of the vaccinia recombinants protected mice against a lethal challenge with alpha-toxin demonstrating that a recombinant vaccinia virus can be used to provide protection against a toxin challenge that is known to be solely antibody mediated.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBacterial Toxins-
dc.subject.meshCalcium-Binding Proteins-
dc.subject.meshCell Line-
dc.subject.meshCercopithecus aethiops-
dc.subject.meshClostridium perfringens-
dc.subject.meshFemale-
dc.subject.meshGenetic Vectors-
dc.subject.meshMice-
dc.subject.meshMice, Inbred BALB C-
dc.subject.meshRecombination, Genetic-
dc.subject.meshType C Phospholipases-
dc.subject.meshVaccinia virus-
dc.titleRecombinant vaccinia viruses protect against Clostridium perfringens alpha-toxin.en
dc.typeArticleen
dc.contributor.departmentDefence Evaluation and Research Agency, Salisbury, Wiltshire, United Kingdom.en
dc.identifier.journalViral Immunologyen

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