Technical report: SYBR Green I and the improved sensitivity of the single-cell electrophoresis assay.

2.50
Hdl Handle:
http://hdl.handle.net/10541/90139
Title:
Technical report: SYBR Green I and the improved sensitivity of the single-cell electrophoresis assay.
Authors:
Ward, Timothy H; Marples, Brian
Abstract:
The single-cell electrophoresis (comet) assay is an established method for measuring radiation-induced strand breaks in DNA. The extent of damage is quantified in individual cells by assessing the intensity and distribution of a fluorescent DNA-binding dye using image analysis software. Using the Kinetic Imaging Komet3 system, it is demonstrated that the fluorochrome SYBR Green I improves the resolution and sensitivity of the comet assay, particularly for measuring radiation-induced double strand breaks.
Affiliation:
Cancer Research Campaign Section of Drug Development and Imaging, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
Citation:
Technical report: SYBR Green I and the improved sensitivity of the single-cell electrophoresis assay. 2000, 76 (1):61-5 Int. J. Radiat. Biol.
Journal:
International Journal of Radiation Biology
Issue Date:
Jan-2000
URI:
http://hdl.handle.net/10541/90139
DOI:
10.1080/095530000139014
PubMed ID:
10665958
Type:
Article
Language:
en
ISSN:
0955-3002
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWard, Timothy Hen
dc.contributor.authorMarples, Brianen
dc.date.accessioned2010-01-20T14:50:15Z-
dc.date.available2010-01-20T14:50:15Z-
dc.date.issued2000-01-
dc.identifier.citationTechnical report: SYBR Green I and the improved sensitivity of the single-cell electrophoresis assay. 2000, 76 (1):61-5 Int. J. Radiat. Biol.en
dc.identifier.issn0955-3002-
dc.identifier.pmid10665958-
dc.identifier.doi10.1080/095530000139014-
dc.identifier.urihttp://hdl.handle.net/10541/90139-
dc.description.abstractThe single-cell electrophoresis (comet) assay is an established method for measuring radiation-induced strand breaks in DNA. The extent of damage is quantified in individual cells by assessing the intensity and distribution of a fluorescent DNA-binding dye using image analysis software. Using the Kinetic Imaging Komet3 system, it is demonstrated that the fluorochrome SYBR Green I improves the resolution and sensitivity of the comet assay, particularly for measuring radiation-induced double strand breaks.en
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subject.meshComet Assay-
dc.subject.meshDNA-
dc.subject.meshDNA Damage-
dc.subject.meshFluorescent Dyes-
dc.subject.meshHumans-
dc.subject.meshImage Processing, Computer-Assisted-
dc.subject.meshOrganic Chemicals-
dc.subject.meshTumor Cells, Cultured-
dc.titleTechnical report: SYBR Green I and the improved sensitivity of the single-cell electrophoresis assay.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Section of Drug Development and Imaging, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.en
dc.identifier.journalInternational Journal of Radiation Biologyen

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