2.50
Hdl Handle:
http://hdl.handle.net/10541/90055
Title:
Donor stromal cells from human blood engraft in NOD/SCID mice.
Authors:
Goan, Silvia-Renate; Junghahn, Ilse; Wissler, Manuela; Becker, Michael; Aumann, Jutta; Just, Ursula; Martiny-Baron, Georg; Fichtner, Iduna; Henschler, Reinhard
Abstract:
Little is known about the presence, frequency, and in vivo proliferative potential of stromal cells within blood-derived hematopoietic transplants. In this study, nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice were injected with human CD34(+) peripheral blood cells (PBCs) or cord blood cells (CBCs, either enriched for CD34 or density-gradient separated mononuclear cells). Flow cytometric analysis 5 to 11 weeks after transplantation revealed the presence of a human lymphomyeloid hematopoiesis within the murine bone marrow. Immunohistochemical staining of bone marrow cell suspensions using human-specific antibodies showed human cells staining positive for human fibroblast markers, human von Willebrand factor (vWF) and human KDR (vascular endothelial growth factor receptor-2) in mice transplanted with CD34(+) PBCs or CBCs, with mean frequencies between 0.6% and 2.4%. In stromal layers of bone marrow cultures established from the mice, immunohistochemical staining using human-specific antibodies revealed flattened reticular cells or spindle-shaped cells staining positive with human-specific antifibroblast antibodies (mean frequency, 2.2%). Cell populations of more rounded cells stained positive with human-specific antibodies recognizing CD34 (1.5%), vWF (2.2%), and KDR (1.6%). Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and subsequent complementary DNA sequencing detected transcripts of human KDR (endothelial specific) and human proline hydroxylase-alpha (fibroblast specific) within the bone marrow and spleen of transplanted mice. Analysis of nontransplanted control mice yielded negative results in immunocytochemistry and RT-PCR. Cells expressing endothelial and fibroblast markers were also detected in the grafts before transplantation, and their numbers increased up to 3 log in vivo after transplantation. These results indicate that stromal progenitor cells are present in human cytokine-mobilized peripheral blood or cord blood that engraft in NOD/SCID mice. (Blood. 2000;96:3971-3978)
Affiliation:
Max Delbrück Center for Molecular Medicine, Berlin, Germany.
Citation:
Donor stromal cells from human blood engraft in NOD/SCID mice. 2000, 96 (12):3971-8 Blood
Journal:
Blood
Issue Date:
1-Dec-2000
URI:
http://hdl.handle.net/10541/90055
PubMed ID:
11090086
Type:
Article
Language:
en
ISSN:
0006-4971
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorGoan, Silvia-Renateen
dc.contributor.authorJunghahn, Ilseen
dc.contributor.authorWissler, Manuelaen
dc.contributor.authorBecker, Michaelen
dc.contributor.authorAumann, Juttaen
dc.contributor.authorJust, Ursulaen
dc.contributor.authorMartiny-Baron, Georgen
dc.contributor.authorFichtner, Idunaen
dc.contributor.authorHenschler, Reinharden
dc.date.accessioned2010-01-19T16:45:40Z-
dc.date.available2010-01-19T16:45:40Z-
dc.date.issued2000-12-01-
dc.identifier.citationDonor stromal cells from human blood engraft in NOD/SCID mice. 2000, 96 (12):3971-8 Blooden
dc.identifier.issn0006-4971-
dc.identifier.pmid11090086-
dc.identifier.urihttp://hdl.handle.net/10541/90055-
dc.description.abstractLittle is known about the presence, frequency, and in vivo proliferative potential of stromal cells within blood-derived hematopoietic transplants. In this study, nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice were injected with human CD34(+) peripheral blood cells (PBCs) or cord blood cells (CBCs, either enriched for CD34 or density-gradient separated mononuclear cells). Flow cytometric analysis 5 to 11 weeks after transplantation revealed the presence of a human lymphomyeloid hematopoiesis within the murine bone marrow. Immunohistochemical staining of bone marrow cell suspensions using human-specific antibodies showed human cells staining positive for human fibroblast markers, human von Willebrand factor (vWF) and human KDR (vascular endothelial growth factor receptor-2) in mice transplanted with CD34(+) PBCs or CBCs, with mean frequencies between 0.6% and 2.4%. In stromal layers of bone marrow cultures established from the mice, immunohistochemical staining using human-specific antibodies revealed flattened reticular cells or spindle-shaped cells staining positive with human-specific antifibroblast antibodies (mean frequency, 2.2%). Cell populations of more rounded cells stained positive with human-specific antibodies recognizing CD34 (1.5%), vWF (2.2%), and KDR (1.6%). Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and subsequent complementary DNA sequencing detected transcripts of human KDR (endothelial specific) and human proline hydroxylase-alpha (fibroblast specific) within the bone marrow and spleen of transplanted mice. Analysis of nontransplanted control mice yielded negative results in immunocytochemistry and RT-PCR. Cells expressing endothelial and fibroblast markers were also detected in the grafts before transplantation, and their numbers increased up to 3 log in vivo after transplantation. These results indicate that stromal progenitor cells are present in human cytokine-mobilized peripheral blood or cord blood that engraft in NOD/SCID mice. (Blood. 2000;96:3971-3978)en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshAntigens, Surface-
dc.subject.meshBone Marrow Cells-
dc.subject.meshCell Culture Techniques-
dc.subject.meshCell Differentiation-
dc.subject.meshEndothelium-
dc.subject.meshFibroblasts-
dc.subject.meshGraft Survival-
dc.subject.meshHematopoiesis-
dc.subject.meshHematopoietic Stem Cell Transplantation-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshImmunophenotyping-
dc.subject.meshMice-
dc.subject.meshMice, Inbred NOD-
dc.subject.meshMice, SCID-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction-
dc.subject.meshStromal Cells-
dc.subject.meshTransplantation, Heterologous-
dc.titleDonor stromal cells from human blood engraft in NOD/SCID mice.en
dc.typeArticleen
dc.contributor.departmentMax Delbrück Center for Molecular Medicine, Berlin, Germany.en
dc.identifier.journalBlooden

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