Insertional mutagenesis as a route to identifying genes involved in self renewal of haemopoietic stem cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/88622
Title:
Insertional mutagenesis as a route to identifying genes involved in self renewal of haemopoietic stem cells.
Authors:
Just, Ursula; Boettiger, D; Kan, O; Dexter, T Michael; Spooncer, Elaine
Abstract:
The genes controlling self renewal in the haemopoietic system are still unknown. Using retroviral insertional mutagenesis we have established multipotent haemopoietic stem cell lines (FDCP-mix) that possess an increased self renewal capacity in vitro. To identify genes involved in the regulation of self renewal, proviral integration sites were cloned from FDCP-mix cells and used as probes to screen independently isolated FDCP-mix cell lines for a common proviral insertion site. So far, two common integration sites have been identified, A25 and M4. A25 is rearranged in 50% of the FDCP-mix cell lines and M4 in 10%. Genes located at or near these sites are likely candidates for the control of self renewal of haemopoietic stem cells.
Affiliation:
Paterson Institute for Cancer Research, Manchester, UK.
Citation:
Insertional mutagenesis as a route to identifying genes involved in self renewal of haemopoietic stem cells. 2000, 251:27-34 Curr. Top. Microbiol. Immunol.
Journal:
Current Topics in Microbiology and Immunology
Issue Date:
2000
URI:
http://hdl.handle.net/10541/88622
PubMed ID:
11036755
Type:
Article
Language:
en
ISSN:
0070-217X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorJust, Ursulaen
dc.contributor.authorBoettiger, Den
dc.contributor.authorKan, Oen
dc.contributor.authorDexter, T Michaelen
dc.contributor.authorSpooncer, Elaineen
dc.date.accessioned2009-12-29T10:48:55Z-
dc.date.available2009-12-29T10:48:55Z-
dc.date.issued2000-
dc.identifier.citationInsertional mutagenesis as a route to identifying genes involved in self renewal of haemopoietic stem cells. 2000, 251:27-34 Curr. Top. Microbiol. Immunol.en
dc.identifier.issn0070-217X-
dc.identifier.pmid11036755-
dc.identifier.urihttp://hdl.handle.net/10541/88622-
dc.description.abstractThe genes controlling self renewal in the haemopoietic system are still unknown. Using retroviral insertional mutagenesis we have established multipotent haemopoietic stem cell lines (FDCP-mix) that possess an increased self renewal capacity in vitro. To identify genes involved in the regulation of self renewal, proviral integration sites were cloned from FDCP-mix cells and used as probes to screen independently isolated FDCP-mix cell lines for a common proviral insertion site. So far, two common integration sites have been identified, A25 and M4. A25 is rearranged in 50% of the FDCP-mix cell lines and M4 in 10%. Genes located at or near these sites are likely candidates for the control of self renewal of haemopoietic stem cells.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshCell Differentiation-
dc.subject.meshCulture Techniques-
dc.subject.meshHelper Viruses-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshMutagenesis, Insertional-
dc.subject.meshRetroviridae-
dc.subject.meshVirus Integration-
dc.titleInsertional mutagenesis as a route to identifying genes involved in self renewal of haemopoietic stem cells.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Manchester, UK.en
dc.identifier.journalCurrent Topics in Microbiology and Immunologyen

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