Mosaic characteristics of human endometrial epithelium in vitro: analysis of secretory markers and cell surface ultrastructure.

2.50
Hdl Handle:
http://hdl.handle.net/10541/88045
Title:
Mosaic characteristics of human endometrial epithelium in vitro: analysis of secretory markers and cell surface ultrastructure.
Authors:
Campbell, S; Larsen, J; Seif, M W; Allen, Terence D; Knox, F; Jones, C J; Aplin, J D
Abstract:
Specific terminal carbohydrate structures and mucin-associated glycans increase in expression within the human endometrial epithelium during the secretory phase of the menstrual cycle but exhibit wide intercellular variation. We postulated that variation in glycosylation between cells would produce differences in the glycocalyx and result in complex mixtures of cells bearing different combinations of glycans. MUC-1 mucin, keratan sulphate and fucosylated lactosaminoglycans were examined in epithelial gland fragment cultures with antibodies (HMFG1, 5D4) and a lectin (Dolichos biflorus agglutinin). The glycocalyx was examined by transmission and high resolution scanning electron microscopy. The data were related to patterns of expression seen in vivo. The MUC-1 mucin was expressed relatively uniformly in culture, but heterogeneity was evident in mucin sialylation within the epithelial cell population. Double labelling of gland explant cultures for combinations of fucosylated lactosaminoglycans, keratan sulphate and MUC-1 demonstrated cells expressing all combinations of these markers. Ultrastructural examination confirmed remarkable intercellular variation in the glycocalyx. Though the human endometrial epithelium is relatively morphologically homogeneous, these observations reveal complex variations of cell surface glycosylation between neighbouring cells and suggest that secretory function might vary in a similar fashion.
Affiliation:
Department of Obstetrics and Gynaecology, University of Glasgow, Royal Infirmary, 10 Alexandra Parade, Glasgow G31 2ER, UK.
Citation:
Mosaic characteristics of human endometrial epithelium in vitro: analysis of secretory markers and cell surface ultrastructure. 2000, 6 (1):41-9 Mol. Hum. Reprod.
Journal:
Molecular Human Reproduction
Issue Date:
Jan-2000
URI:
http://hdl.handle.net/10541/88045
PubMed ID:
10611259
Type:
Article
Language:
en
ISSN:
1360-9947
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorCampbell, Sen
dc.contributor.authorLarsen, Jen
dc.contributor.authorSeif, M Wen
dc.contributor.authorAllen, Terence Den
dc.contributor.authorKnox, Fen
dc.contributor.authorJones, C Jen
dc.contributor.authorAplin, J Den
dc.date.accessioned2009-12-15T17:12:14Z-
dc.date.available2009-12-15T17:12:14Z-
dc.date.issued2000-01-
dc.identifier.citationMosaic characteristics of human endometrial epithelium in vitro: analysis of secretory markers and cell surface ultrastructure. 2000, 6 (1):41-9 Mol. Hum. Reprod.en
dc.identifier.issn1360-9947-
dc.identifier.pmid10611259-
dc.identifier.urihttp://hdl.handle.net/10541/88045-
dc.description.abstractSpecific terminal carbohydrate structures and mucin-associated glycans increase in expression within the human endometrial epithelium during the secretory phase of the menstrual cycle but exhibit wide intercellular variation. We postulated that variation in glycosylation between cells would produce differences in the glycocalyx and result in complex mixtures of cells bearing different combinations of glycans. MUC-1 mucin, keratan sulphate and fucosylated lactosaminoglycans were examined in epithelial gland fragment cultures with antibodies (HMFG1, 5D4) and a lectin (Dolichos biflorus agglutinin). The glycocalyx was examined by transmission and high resolution scanning electron microscopy. The data were related to patterns of expression seen in vivo. The MUC-1 mucin was expressed relatively uniformly in culture, but heterogeneity was evident in mucin sialylation within the epithelial cell population. Double labelling of gland explant cultures for combinations of fucosylated lactosaminoglycans, keratan sulphate and MUC-1 demonstrated cells expressing all combinations of these markers. Ultrastructural examination confirmed remarkable intercellular variation in the glycocalyx. Though the human endometrial epithelium is relatively morphologically homogeneous, these observations reveal complex variations of cell surface glycosylation between neighbouring cells and suggest that secretory function might vary in a similar fashion.en
dc.language.isoenen
dc.subject.meshAdult-
dc.subject.meshAmino Sugars-
dc.subject.meshBiological Markers-
dc.subject.meshCell Membrane-
dc.subject.meshCells, Cultured-
dc.subject.meshEndometrium-
dc.subject.meshEpithelial Cells-
dc.subject.meshEpithelium-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshKeratan Sulfate-
dc.subject.meshLectins-
dc.subject.meshMicroscopy, Electron-
dc.subject.meshMicroscopy, Electron, Scanning-
dc.subject.meshMiddle Aged-
dc.subject.meshMucin-1-
dc.subject.meshPeptide Fragments-
dc.subject.meshPlant Lectins-
dc.subject.meshPolysaccharides-
dc.titleMosaic characteristics of human endometrial epithelium in vitro: analysis of secretory markers and cell surface ultrastructure.en
dc.typeArticleen
dc.contributor.departmentDepartment of Obstetrics and Gynaecology, University of Glasgow, Royal Infirmary, 10 Alexandra Parade, Glasgow G31 2ER, UK.en
dc.identifier.journalMolecular Human Reproductionen

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