A correlation between residual radiation-induced DNA double-strand breaks in cultured fibroblasts and late radiotherapy reactions in breast cancer patients.

2.50
Hdl Handle:
http://hdl.handle.net/10541/88034
Title:
A correlation between residual radiation-induced DNA double-strand breaks in cultured fibroblasts and late radiotherapy reactions in breast cancer patients.
Authors:
Kiltie, Anne E; Ryan, Anderson J; Swindell, Ric; Barber, James B P; West, Catharine M L; Magee, Brian; Hendry, Jolyon H
Abstract:
BACKGROUND AND PURPOSE: Prediction of late normal tissue reactions to radiotherapy would permit tailoring of dosage to each patient. Measurement of residual DNA double strand breaks using pulsed field gel electrophoresis (PFGE) shows promise in this field. The aim of this study was to test the predictive potential of PFGE in a group of retrospectively studied breast cancer patients. MATERIALS AND METHODS: Thirty nine patients, treated uniformly for breast cancer 9-15 years previously, with excision of the tumour and radiotherapy to the breast and drainage areas, were assessed clinically using the LENT SOMA scale, and a 5-mm punch biopsy taken from the buttock. Fibroblast cell strains were established and used to study residual DNA double strand breaks, using PFGE. RESULTS: There were significant correlations between the DNA assay results and the fibrosis score (r(s) = 0.46; P = 0.003), the combined fibrosis and retraction score (r(s) = 0.45, P = 0.004) and the overall LENT score (r(s) = 0.43; P = 0.006). Using polychotomous logistic regression, the fibroblast DNA assay result was an independent prognostic factor for fibrosis severity. CONCLUSIONS: There is a relationship between residual radiation-induced DNA damage in fibroblasts and the severity of the late normal tissue damage seen in the patients from whom the cells were cultured.
Affiliation:
CRC Section of Genome Damage and Repair, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Citation:
A correlation between residual radiation-induced DNA double-strand breaks in cultured fibroblasts and late radiotherapy reactions in breast cancer patients. 1999, 51 (1):55-65 Radiother Oncol
Journal:
Radiotherapy and Oncology
Issue Date:
Apr-1999
URI:
http://hdl.handle.net/10541/88034
DOI:
10.1016/S0167-8140(99)00030-4
PubMed ID:
10386717
Type:
Article
Language:
en
ISSN:
0167-8140
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorKiltie, Anne Een
dc.contributor.authorRyan, Anderson Jen
dc.contributor.authorSwindell, Ricen
dc.contributor.authorBarber, James B Pen
dc.contributor.authorWest, Catharine M Len
dc.contributor.authorMagee, Brianen
dc.contributor.authorHendry, Jolyon Hen
dc.date.accessioned2009-12-15T16:13:39Z-
dc.date.available2009-12-15T16:13:39Z-
dc.date.issued1999-04-
dc.identifier.citationA correlation between residual radiation-induced DNA double-strand breaks in cultured fibroblasts and late radiotherapy reactions in breast cancer patients. 1999, 51 (1):55-65 Radiother Oncolen
dc.identifier.issn0167-8140-
dc.identifier.pmid10386717-
dc.identifier.doi10.1016/S0167-8140(99)00030-4-
dc.identifier.urihttp://hdl.handle.net/10541/88034-
dc.description.abstractBACKGROUND AND PURPOSE: Prediction of late normal tissue reactions to radiotherapy would permit tailoring of dosage to each patient. Measurement of residual DNA double strand breaks using pulsed field gel electrophoresis (PFGE) shows promise in this field. The aim of this study was to test the predictive potential of PFGE in a group of retrospectively studied breast cancer patients. MATERIALS AND METHODS: Thirty nine patients, treated uniformly for breast cancer 9-15 years previously, with excision of the tumour and radiotherapy to the breast and drainage areas, were assessed clinically using the LENT SOMA scale, and a 5-mm punch biopsy taken from the buttock. Fibroblast cell strains were established and used to study residual DNA double strand breaks, using PFGE. RESULTS: There were significant correlations between the DNA assay results and the fibrosis score (r(s) = 0.46; P = 0.003), the combined fibrosis and retraction score (r(s) = 0.45, P = 0.004) and the overall LENT score (r(s) = 0.43; P = 0.006). Using polychotomous logistic regression, the fibroblast DNA assay result was an independent prognostic factor for fibrosis severity. CONCLUSIONS: There is a relationship between residual radiation-induced DNA damage in fibroblasts and the severity of the late normal tissue damage seen in the patients from whom the cells were cultured.en
dc.language.isoenen
dc.subjectBreast Canceren
dc.subject.meshBreast Neoplasms-
dc.subject.meshDNA-
dc.subject.meshDNA Damage-
dc.subject.meshElectrophoresis, Gel, Pulsed-Field-
dc.subject.meshFibroblasts-
dc.subject.meshFibrosis-
dc.subject.meshHumans-
dc.subject.meshRadiation Injuries-
dc.subject.meshRadiotherapy-
dc.subject.meshRetrospective Studies-
dc.titleA correlation between residual radiation-induced DNA double-strand breaks in cultured fibroblasts and late radiotherapy reactions in breast cancer patients.en
dc.typeArticleen
dc.contributor.departmentCRC Section of Genome Damage and Repair, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.en
dc.identifier.journalRadiotherapy and Oncologyen
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