Myeloid- and lymphoid-specific breakpoint cluster regions in chromosome band 13q14 in acute leukemia.

2.50
Hdl Handle:
http://hdl.handle.net/10541/88018
Title:
Myeloid- and lymphoid-specific breakpoint cluster regions in chromosome band 13q14 in acute leukemia.
Authors:
Coignet, Lionel J; Lima, Carmen S; Min, Toon Min; Streubel, Berthold; Swansbury, John; Telford, Nicholas; Swanton, Soheila; Bowen, Angela; Nagai, Masami; Catovsky, Daniel; Fonatsch, Christa; Dyer, Martin J
Abstract:
Abnormalities of chromosome band 13q14 occur in hematologic malignancies of all lineages and at all stages of differentiation. Unlike other chromosomal translocations, which are usually specific for a given lineage, the chromosomal translocation t(12;13)(p12;q14) has been observed in both B-cell and T-cell precursor acute lymphoblastic leukemia (BCP-, TCP-ALL), in differentiated and undifferentiated acute myeloblastic leukemia (AML), and in chronic myeloid leukemia (CML) at progression to blast crisis. The nature of these translocations and their pathologic consequences remain unknown. To begin to define the gene(s) involved on chromosome 13, we have performed fluorescence in situ hybridization (FISH) using a panel of YACs from the region, on a series of 10 cases of acute leukemia with t(12;13)(p12;q14) and 1 case each with "variant" translocations including t(12;13)(q21;q14), t(10;13)(q24;q14) and t(9;13)(p21;q14). In 8/13 cases/cell lines, the 13q14 break fell within a single 1.4 Mb CEPH MegaYAC. This YAC fell immediately telomeric of the forkhead (FKHR) gene, which is disrupted in the t(2;13)(q35;q14) seen in pediatric alveolar rhabdomyosarcoma. Seven of the 8 cases with breaks in this YAC were AML. In 4/13 cases, the 13q14 break fell within a 1.7-Mb YAC located about 3 Mb telomeric of the retinoblastoma (RB1) gene: all 4 cases were ALL. One case of myelodysplastic syndrome exhibited a break within 13q12, adjacent to the BRCA2 gene. These data indicate the presence of myeloid- and lymphoid-specific breakpoint cluster regions within chromosome band 13q14 in acute leukemia.
Affiliation:
The Academic Department of Haematology and Cytogenetics, Institute of Cancer Research, Royal Marsden Hospital, Sutton, Surrey, United Kingdom.
Citation:
Myeloid- and lymphoid-specific breakpoint cluster regions in chromosome band 13q14 in acute leukemia. 1999, 25 (3):222-9 Genes Chromosomes Cancer
Journal:
Genes, Chromosomes & Cancer
Issue Date:
Jul-1999
URI:
http://hdl.handle.net/10541/88018
DOI:
10.1002/(SICI)1098-2264(199907)25:3<222::AID-GCC4>3.0.CO;2-C
PubMed ID:
10379868
Type:
Article
Language:
en
ISSN:
1045-2257
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorCoignet, Lionel Jen
dc.contributor.authorLima, Carmen Sen
dc.contributor.authorMin, Toon Minen
dc.contributor.authorStreubel, Bertholden
dc.contributor.authorSwansbury, Johnen
dc.contributor.authorTelford, Nicholasen
dc.contributor.authorSwanton, Soheilaen
dc.contributor.authorBowen, Angelaen
dc.contributor.authorNagai, Masamien
dc.contributor.authorCatovsky, Danielen
dc.contributor.authorFonatsch, Christaen
dc.contributor.authorDyer, Martin Jen
dc.date.accessioned2009-12-15T15:25:19Z-
dc.date.available2009-12-15T15:25:19Z-
dc.date.issued1999-07-
dc.identifier.citationMyeloid- and lymphoid-specific breakpoint cluster regions in chromosome band 13q14 in acute leukemia. 1999, 25 (3):222-9 Genes Chromosomes Canceren
dc.identifier.issn1045-2257-
dc.identifier.pmid10379868-
dc.identifier.doi10.1002/(SICI)1098-2264(199907)25:3<222::AID-GCC4>3.0.CO;2-C-
dc.identifier.urihttp://hdl.handle.net/10541/88018-
dc.description.abstractAbnormalities of chromosome band 13q14 occur in hematologic malignancies of all lineages and at all stages of differentiation. Unlike other chromosomal translocations, which are usually specific for a given lineage, the chromosomal translocation t(12;13)(p12;q14) has been observed in both B-cell and T-cell precursor acute lymphoblastic leukemia (BCP-, TCP-ALL), in differentiated and undifferentiated acute myeloblastic leukemia (AML), and in chronic myeloid leukemia (CML) at progression to blast crisis. The nature of these translocations and their pathologic consequences remain unknown. To begin to define the gene(s) involved on chromosome 13, we have performed fluorescence in situ hybridization (FISH) using a panel of YACs from the region, on a series of 10 cases of acute leukemia with t(12;13)(p12;q14) and 1 case each with "variant" translocations including t(12;13)(q21;q14), t(10;13)(q24;q14) and t(9;13)(p21;q14). In 8/13 cases/cell lines, the 13q14 break fell within a single 1.4 Mb CEPH MegaYAC. This YAC fell immediately telomeric of the forkhead (FKHR) gene, which is disrupted in the t(2;13)(q35;q14) seen in pediatric alveolar rhabdomyosarcoma. Seven of the 8 cases with breaks in this YAC were AML. In 4/13 cases, the 13q14 break fell within a 1.7-Mb YAC located about 3 Mb telomeric of the retinoblastoma (RB1) gene: all 4 cases were ALL. One case of myelodysplastic syndrome exhibited a break within 13q12, adjacent to the BRCA2 gene. These data indicate the presence of myeloid- and lymphoid-specific breakpoint cluster regions within chromosome band 13q14 in acute leukemia.en
dc.language.isoenen
dc.subjectLeukaemiaen
dc.subjectPrecursor Cell Lymphoblastic Leukaemia-Lymphomaen
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshChild-
dc.subject.meshChild, Preschool-
dc.subject.meshChromosome Breakage-
dc.subject.meshChromosomes, Human, Pair 13-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshIn Situ Hybridization, Fluorescence-
dc.subject.meshInfant-
dc.subject.meshLeukemia, Monocytic, Acute-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshPrecursor Cell Lymphoblastic Leukemia-Lymphoma-
dc.subject.meshTranslocation, Genetic-
dc.titleMyeloid- and lymphoid-specific breakpoint cluster regions in chromosome band 13q14 in acute leukemia.en
dc.typeArticleen
dc.contributor.departmentThe Academic Department of Haematology and Cytogenetics, Institute of Cancer Research, Royal Marsden Hospital, Sutton, Surrey, United Kingdom.en
dc.identifier.journalGenes, Chromosomes & Canceren

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