Scatter factor influences the formation of prostate epithelial cell colonies on bone marrow stroma in vitro.

2.50
Hdl Handle:
http://hdl.handle.net/10541/88012
Title:
Scatter factor influences the formation of prostate epithelial cell colonies on bone marrow stroma in vitro.
Authors:
Lang, Shona H; Clarke, Noel W ( 0000-0001-7776-8059 ) ; George, Nicholas J; Testa, Nydia G
Abstract:
Prostate cancer metastases form selectively in the bone marrow. Previously we demonstrated motility was important for the formation of primary prostatic epithelial cell colonies in bone marrow stroma (BMS) co-culture. In this study we looked at the influence of motility factors on the colony formation of epithelial cells derived from benign (bPEC) or malignant (mPEC) prostate tissue. After 7 days co-culture we found that anti-scatter factor consistently inhibited prostate epithelial cell colony formation on BMS (7/7 mPEC and 4/7 bPEC samples showed significant inhibition). Antibodies against bFGF and 5T4 did not significantly affect colony formation. Addition of fibroblast conditioned media (derived from benign prostates) to co-cultures stimulated the colony formation of bPEC (170%) and mPEC (252%). This stimulation was eliminated by depletion of SF from the conditioned media. Immunohistochemical staining found c-Met expression in 5/6 bPEC cultures and 7/9 mPEC cultures. When grown in BMS co-culture expression of c-Met was positive in 3/6 bPEC and 2/7 mPEC samples. In conclusion, scatter factor influences the in vitro formation of prostate epithelial cell colonies on BMS co-culture.
Affiliation:
CRC Section of Haemopoietic Cell and Gene Therapeutics, Patterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK. shl2@imap.york.ac.uk
Citation:
Scatter factor influences the formation of prostate epithelial cell colonies on bone marrow stroma in vitro. 1999, 17 (4):333-40 Clin. Exp. Metastasis
Journal:
Clinical & Experimental Metastasis
Issue Date:
Jun-1999
URI:
http://hdl.handle.net/10541/88012
DOI:
10.1023/A:1006696002497
PubMed ID:
10545020
Type:
Article
Language:
en
ISSN:
0262-0898
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLang, Shona Hen
dc.contributor.authorClarke, Noel Wen
dc.contributor.authorGeorge, Nicholas Jen
dc.contributor.authorTesta, Nydia Gen
dc.date.accessioned2009-12-15T16:28:27Z-
dc.date.available2009-12-15T16:28:27Z-
dc.date.issued1999-06-
dc.identifier.citationScatter factor influences the formation of prostate epithelial cell colonies on bone marrow stroma in vitro. 1999, 17 (4):333-40 Clin. Exp. Metastasisen
dc.identifier.issn0262-0898-
dc.identifier.pmid10545020-
dc.identifier.doi10.1023/A:1006696002497-
dc.identifier.urihttp://hdl.handle.net/10541/88012-
dc.description.abstractProstate cancer metastases form selectively in the bone marrow. Previously we demonstrated motility was important for the formation of primary prostatic epithelial cell colonies in bone marrow stroma (BMS) co-culture. In this study we looked at the influence of motility factors on the colony formation of epithelial cells derived from benign (bPEC) or malignant (mPEC) prostate tissue. After 7 days co-culture we found that anti-scatter factor consistently inhibited prostate epithelial cell colony formation on BMS (7/7 mPEC and 4/7 bPEC samples showed significant inhibition). Antibodies against bFGF and 5T4 did not significantly affect colony formation. Addition of fibroblast conditioned media (derived from benign prostates) to co-cultures stimulated the colony formation of bPEC (170%) and mPEC (252%). This stimulation was eliminated by depletion of SF from the conditioned media. Immunohistochemical staining found c-Met expression in 5/6 bPEC cultures and 7/9 mPEC cultures. When grown in BMS co-culture expression of c-Met was positive in 3/6 bPEC and 2/7 mPEC samples. In conclusion, scatter factor influences the in vitro formation of prostate epithelial cell colonies on BMS co-culture.en
dc.language.isoenen
dc.subject.meshBone Marrow Cells-
dc.subject.meshCell Division-
dc.subject.meshCoculture Techniques-
dc.subject.meshCulture Media, Conditioned-
dc.subject.meshEpithelial Cells-
dc.subject.meshFibroblast Growth Factor 2-
dc.subject.meshHepatocyte Growth Factor-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshProstate-
dc.subject.meshProto-Oncogene Proteins c-met-
dc.subject.meshRecombinant Proteins-
dc.subject.meshStromal Cells-
dc.titleScatter factor influences the formation of prostate epithelial cell colonies on bone marrow stroma in vitro.en
dc.typeArticleen
dc.contributor.departmentCRC Section of Haemopoietic Cell and Gene Therapeutics, Patterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK. shl2@imap.york.ac.uken
dc.identifier.journalClinical & Experimental Metastasisen

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