Content of long-term culture-initiating cells, clonogenic progenitors and CD34 cells in apheresis harvests of normal donors for allogeneic transplantation, and in patients with acute myeloid leukaemia or multiple myeloma.

2.50
Hdl Handle:
http://hdl.handle.net/10541/88010
Title:
Content of long-term culture-initiating cells, clonogenic progenitors and CD34 cells in apheresis harvests of normal donors for allogeneic transplantation, and in patients with acute myeloid leukaemia or multiple myeloma.
Authors:
Kasper, C; Ryder, W David J; Dürig, J; Nagesh, K; Scarffe, J Howard; Beelen, D W; Schaefer, U W; Chang, James; Testa, Nydia G
Abstract:
Using a limiting dilution assay the frequency of long-term culture-initiating cells (LTC-IC) in the apheresis products following mobilization by granulocyte-colony stimulating factor (G-CSP) with or without chemotherapy from 14 normal donors (ND) for allogeneic bone marrow transplantation, 16 patients with multiple myeloma (MM) and 15 patients with acute myeloid leukaemia (AML), where the aphereses were intended for autologous transplantation, were compared. The estimated median incidences of LTC-IC in the first apheresis products from ND, MM and AML were 1/3289, 1/1775 and 1/13075 mononuclear cells (MNC) respectively. The patients with AML had a significantly lower incidence compared with the other two groups (P < 0.0001). There was a positive correlation between the incidence of LTC-IC and the number of CD34+ cells, the number of GM-CFC, and the number of BFU-E. The positive association with GM-CFC or BFU-E was weaker. In these experiments the percentage of CD34+ cells was the best predictor for the frequency of LTC-IC in the peripheral blood progenitor cells (PBPC). In eight cases of MM the LTC-IC assay was performed for both the first and second harvest. All cases had a lower LTC-IC frequency in the second harvest compared with the first, an average of 23% (13-42%, 95% confidence interval) and this reduction was statistically significant (P<0 001); CD34+ cells were also lower (P< 0.001).
Affiliation:
Cancer Research Campaign Department of Experimental Haematology, Christie Hospital, Manchester, UK.
Citation:
Content of long-term culture-initiating cells, clonogenic progenitors and CD34 cells in apheresis harvests of normal donors for allogeneic transplantation, and in patients with acute myeloid leukaemia or multiple myeloma. 1999, 104 (2):374-81 Br. J. Haematol.
Journal:
British Journal of Haematology
Issue Date:
Feb-1999
URI:
http://hdl.handle.net/10541/88010
DOI:
10.1046/j.1365-2141.1999.01152.x
PubMed ID:
10050722
Type:
Article
Language:
en
ISSN:
0007-1048
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorKasper, Cen
dc.contributor.authorRyder, W David Jen
dc.contributor.authorDürig, Jen
dc.contributor.authorNagesh, Ken
dc.contributor.authorScarffe, J Howarden
dc.contributor.authorBeelen, D Wen
dc.contributor.authorSchaefer, U Wen
dc.contributor.authorChang, Jamesen
dc.contributor.authorTesta, Nydia Gen
dc.date.accessioned2009-12-15T16:20:24Z-
dc.date.available2009-12-15T16:20:24Z-
dc.date.issued1999-02-
dc.identifier.citationContent of long-term culture-initiating cells, clonogenic progenitors and CD34 cells in apheresis harvests of normal donors for allogeneic transplantation, and in patients with acute myeloid leukaemia or multiple myeloma. 1999, 104 (2):374-81 Br. J. Haematol.en
dc.identifier.issn0007-1048-
dc.identifier.pmid10050722-
dc.identifier.doi10.1046/j.1365-2141.1999.01152.x-
dc.identifier.urihttp://hdl.handle.net/10541/88010-
dc.description.abstractUsing a limiting dilution assay the frequency of long-term culture-initiating cells (LTC-IC) in the apheresis products following mobilization by granulocyte-colony stimulating factor (G-CSP) with or without chemotherapy from 14 normal donors (ND) for allogeneic bone marrow transplantation, 16 patients with multiple myeloma (MM) and 15 patients with acute myeloid leukaemia (AML), where the aphereses were intended for autologous transplantation, were compared. The estimated median incidences of LTC-IC in the first apheresis products from ND, MM and AML were 1/3289, 1/1775 and 1/13075 mononuclear cells (MNC) respectively. The patients with AML had a significantly lower incidence compared with the other two groups (P < 0.0001). There was a positive correlation between the incidence of LTC-IC and the number of CD34+ cells, the number of GM-CFC, and the number of BFU-E. The positive association with GM-CFC or BFU-E was weaker. In these experiments the percentage of CD34+ cells was the best predictor for the frequency of LTC-IC in the peripheral blood progenitor cells (PBPC). In eight cases of MM the LTC-IC assay was performed for both the first and second harvest. All cases had a lower LTC-IC frequency in the second harvest compared with the first, an average of 23% (13-42%, 95% confidence interval) and this reduction was statistically significant (P<0 001); CD34+ cells were also lower (P< 0.001).en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cell Mobilisationen
dc.subjectHaematopoietic Stem Cell Transplantationen
dc.subjectMyeloid Leukaemiaen
dc.subject.meshAcute Disease-
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAntigens, CD34-
dc.subject.meshBlood Component Removal-
dc.subject.meshBlood Donors-
dc.subject.meshErythroid Precursor Cells-
dc.subject.meshFemale-
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor-
dc.subject.meshHematopoietic Stem Cell Mobilization-
dc.subject.meshHematopoietic Stem Cell Transplantation-
dc.subject.meshHumans-
dc.subject.meshLeukemia, Myeloid-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshMultiple Myeloma-
dc.subject.meshTransplantation, Homologous-
dc.titleContent of long-term culture-initiating cells, clonogenic progenitors and CD34 cells in apheresis harvests of normal donors for allogeneic transplantation, and in patients with acute myeloid leukaemia or multiple myeloma.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Experimental Haematology, Christie Hospital, Manchester, UK.en
dc.identifier.journalBritish Journal of Haematologyen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.