The lack of correlation between proliferation (Ki-67, PCNA, LI, Tpot), p53 expression and radiosensitivity for head and neck cancers.

2.50
Hdl Handle:
http://hdl.handle.net/10541/87857
Title:
The lack of correlation between proliferation (Ki-67, PCNA, LI, Tpot), p53 expression and radiosensitivity for head and neck cancers.
Authors:
Björk-Eriksson, Thomas; West, Catharine M L; Cvetskovska, E; Svensson, M; Karlsson, E; Magnusson, Bengt; Slevin, Nicholas J ( 0000-0002-3367-7013 ) ; Edström, S; Mercke, Claes
Abstract:
A study was made of the relationship between measurements of radiosensitivity versus proliferation and p53 status in head and neck cancers. Inherent tumour radiosensitivity was assessed as surviving fraction at 2 Gy (SF2) using a clonogenic soft agar assay (n = 77). The results were compared to data on proliferation obtained by both flow cytometry (labelling index (LI), the potential doubling time (Tpot) n = 55) and immunohistochemistry (Ki-67 and PCNA; n = 68), together with immunohistochemical p53 expression (n = 68). There were no overall significant differences in the median values of the various parameters analysed for the different sites within the head and neck region, disease stages, grades of tumour differentiation or nodal states. A subgroup analysis showed that oropharyngeal (n = 22) versus oral cavity (n = 35) tumours were more radiosensitive (P = 0.056) and had a higher Ki-67 index (P = 0.001). Node-positive tumours had higher LI (P = 0.021) and a trend towards lower Tpot (P = 0.067) values than node-negative ones. No correlations were seen between SF2 and any of the parameters studied. The long-standing dogma of an increased radiosensitivity of rapidly proliferating cells in contrast to slowly proliferating cells was not confirmed. The study shows that parallel measurements of different biological markers can be obtained for a large number of patients with head and neck cancers. The independence of the various parameters studied suggests that there may be potential for their combined use as prognostic factors for the outcome of radiotherapy.
Affiliation:
Department of Oncology, Sahlgrenska University Hospital, Gothenburg, Sweden.
Citation:
The lack of correlation between proliferation (Ki-67, PCNA, LI, Tpot), p53 expression and radiosensitivity for head and neck cancers. 1999, 80 (9):1400-4 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
Jul-1999
URI:
http://hdl.handle.net/10541/87857
DOI:
10.1038/sj.bjc.6690535
PubMed ID:
10424742
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorBjörk-Eriksson, Thomasen
dc.contributor.authorWest, Catharine M Len
dc.contributor.authorCvetskovska, Een
dc.contributor.authorSvensson, Men
dc.contributor.authorKarlsson, Een
dc.contributor.authorMagnusson, Bengten
dc.contributor.authorSlevin, Nicholas Jen
dc.contributor.authorEdström, Sen
dc.contributor.authorMercke, Claesen
dc.date.accessioned2009-12-14T12:23:46Z-
dc.date.available2009-12-14T12:23:46Z-
dc.date.issued1999-07-
dc.identifier.citationThe lack of correlation between proliferation (Ki-67, PCNA, LI, Tpot), p53 expression and radiosensitivity for head and neck cancers. 1999, 80 (9):1400-4 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid10424742-
dc.identifier.doi10.1038/sj.bjc.6690535-
dc.identifier.urihttp://hdl.handle.net/10541/87857-
dc.description.abstractA study was made of the relationship between measurements of radiosensitivity versus proliferation and p53 status in head and neck cancers. Inherent tumour radiosensitivity was assessed as surviving fraction at 2 Gy (SF2) using a clonogenic soft agar assay (n = 77). The results were compared to data on proliferation obtained by both flow cytometry (labelling index (LI), the potential doubling time (Tpot) n = 55) and immunohistochemistry (Ki-67 and PCNA; n = 68), together with immunohistochemical p53 expression (n = 68). There were no overall significant differences in the median values of the various parameters analysed for the different sites within the head and neck region, disease stages, grades of tumour differentiation or nodal states. A subgroup analysis showed that oropharyngeal (n = 22) versus oral cavity (n = 35) tumours were more radiosensitive (P = 0.056) and had a higher Ki-67 index (P = 0.001). Node-positive tumours had higher LI (P = 0.021) and a trend towards lower Tpot (P = 0.067) values than node-negative ones. No correlations were seen between SF2 and any of the parameters studied. The long-standing dogma of an increased radiosensitivity of rapidly proliferating cells in contrast to slowly proliferating cells was not confirmed. The study shows that parallel measurements of different biological markers can be obtained for a large number of patients with head and neck cancers. The independence of the various parameters studied suggests that there may be potential for their combined use as prognostic factors for the outcome of radiotherapy.en
dc.language.isoenen
dc.subjectHead and Neck Canceren
dc.subjectTumour Suppressor Protein p53en
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAged, 80 and over-
dc.subject.meshCell Division-
dc.subject.meshDNA-
dc.subject.meshFemale-
dc.subject.meshHead and Neck Neoplasms-
dc.subject.meshHumans-
dc.subject.meshKi-67 Antigen-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshProliferating Cell Nuclear Antigen-
dc.subject.meshRadiation Tolerance-
dc.subject.meshTumor Suppressor Protein p53-
dc.titleThe lack of correlation between proliferation (Ki-67, PCNA, LI, Tpot), p53 expression and radiosensitivity for head and neck cancers.en
dc.typeArticleen
dc.contributor.departmentDepartment of Oncology, Sahlgrenska University Hospital, Gothenburg, Sweden.en
dc.identifier.journalBritish Journal of Canceren

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