The immunohistochemical expression of DNA-PKCS and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivity.

2.50
Hdl Handle:
http://hdl.handle.net/10541/87855
Title:
The immunohistochemical expression of DNA-PKCS and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivity.
Authors:
Björk-Eriksson, Thomas; West, Catharine M L; Nilsson, Anders; Magnusson, Bengt; Svensson, Marie; Karlsson, Ewa; Slevin, Nicholas J ( 0000-0002-3367-7013 ) ; Lewensohn, Rolf; Mercke, Claes
Abstract:
PURPOSE: The DNA-PK complex is one of the major pathways by which mammalian cells respond to DNA double-strand breaks induced by ionizing radiation. This study evaluated the relationship between the immunohistochemical expression of the individual components of DNA-PK and cellular radiosensitivity in head and neck cancers. METHODS AND MATERIALS: Biopsies from patients with previously untreated squamous cell carcinomas of the head and neck were assessed for inherent tumor radiosensitivity measured as the surviving fraction at 2 Gy (SF2) using a soft agar clonogenic assay. Paraffin-embedded tumor material from 64 successfully grown specimens was immunohistochemically stained for expression of DNA-PKcs and Ku (p70/p80). The same tumor material was previously analyzed for the immunohistochemical expression of p53. RESULTS: A significant correlation was found between the degree of expression of DNA-PKcs and Ku (p70/p80) (r = 0.55, p<0.001). There were no overall significant differences in the levels of expression of DNA-PKcs and Ku (p70/p80) in tumors from patients of either sex, different sites, histologies, and stages. No relationship was found between SF2 and the expression of either DNA-PKcs (r = 0.22, p = 0.081) or Ku (p70/p80) (r = 0.064, p = 0.62). Comparison with previous immunohistochemical characterization showed no significant correlations between the expression levels of p53 and either DNA-PKcs (r = 0.093, p = 0.46) or Ku (p70/p80) (r = -0.17, p = 0.17). CONCLUSIONS: This study suggests that determining the immunohistochemical expression of DNA-PK in head and neck cancers from multiple sites does not have a role as a predictive assay of tumor in vitro radiosensitivity.
Affiliation:
Department of Oncology, Sahlgrenska University Hospital, Gothenburg, Sweden. thomas.bjork-eriksson@oncology.gu.se
Citation:
The immunohistochemical expression of DNA-PKCS and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivity. 1999, 45 (4):1005-10 Int. J. Radiat. Oncol. Biol. Phys.
Journal:
International Journal of Radiation Oncology, Biology, Physics
Issue Date:
1-Nov-1999
URI:
http://hdl.handle.net/10541/87855
PubMed ID:
10571209
Type:
Article
Language:
en
ISSN:
0360-3016
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorBjörk-Eriksson, Thomasen
dc.contributor.authorWest, Catharine M Len
dc.contributor.authorNilsson, Andersen
dc.contributor.authorMagnusson, Bengten
dc.contributor.authorSvensson, Marieen
dc.contributor.authorKarlsson, Ewaen
dc.contributor.authorSlevin, Nicholas Jen
dc.contributor.authorLewensohn, Rolfen
dc.contributor.authorMercke, Claesen
dc.date.accessioned2009-12-14T12:16:04Z-
dc.date.available2009-12-14T12:16:04Z-
dc.date.issued1999-11-01-
dc.identifier.citationThe immunohistochemical expression of DNA-PKCS and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivity. 1999, 45 (4):1005-10 Int. J. Radiat. Oncol. Biol. Phys.en
dc.identifier.issn0360-3016-
dc.identifier.pmid10571209-
dc.identifier.urihttp://hdl.handle.net/10541/87855-
dc.description.abstractPURPOSE: The DNA-PK complex is one of the major pathways by which mammalian cells respond to DNA double-strand breaks induced by ionizing radiation. This study evaluated the relationship between the immunohistochemical expression of the individual components of DNA-PK and cellular radiosensitivity in head and neck cancers. METHODS AND MATERIALS: Biopsies from patients with previously untreated squamous cell carcinomas of the head and neck were assessed for inherent tumor radiosensitivity measured as the surviving fraction at 2 Gy (SF2) using a soft agar clonogenic assay. Paraffin-embedded tumor material from 64 successfully grown specimens was immunohistochemically stained for expression of DNA-PKcs and Ku (p70/p80). The same tumor material was previously analyzed for the immunohistochemical expression of p53. RESULTS: A significant correlation was found between the degree of expression of DNA-PKcs and Ku (p70/p80) (r = 0.55, p<0.001). There were no overall significant differences in the levels of expression of DNA-PKcs and Ku (p70/p80) in tumors from patients of either sex, different sites, histologies, and stages. No relationship was found between SF2 and the expression of either DNA-PKcs (r = 0.22, p = 0.081) or Ku (p70/p80) (r = 0.064, p = 0.62). Comparison with previous immunohistochemical characterization showed no significant correlations between the expression levels of p53 and either DNA-PKcs (r = 0.093, p = 0.46) or Ku (p70/p80) (r = -0.17, p = 0.17). CONCLUSIONS: This study suggests that determining the immunohistochemical expression of DNA-PK in head and neck cancers from multiple sites does not have a role as a predictive assay of tumor in vitro radiosensitivity.en
dc.language.isoenen
dc.subjectCancer DNAen
dc.subjectCancer Proteinsen
dc.subjectHead and Neck Canceren
dc.subject.meshAntigens, Nuclear-
dc.subject.meshCalcium-Binding Proteins-
dc.subject.meshDNA Helicases-
dc.subject.meshDNA, Neoplasm-
dc.subject.meshDNA-Binding Proteins-
dc.subject.meshFemale-
dc.subject.meshHead and Neck Neoplasms-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshMale-
dc.subject.meshNeoplasm Proteins-
dc.subject.meshNuclear Proteins-
dc.subject.meshRadiation Tolerance-
dc.titleThe immunohistochemical expression of DNA-PKCS and Ku (p70/p80) in head and neck cancers: relationships with radiosensitivity.en
dc.typeArticleen
dc.contributor.departmentDepartment of Oncology, Sahlgrenska University Hospital, Gothenburg, Sweden. thomas.bjork-eriksson@oncology.gu.seen
dc.identifier.journalInternational Journal of Radiation Oncology, Biology, Physicsen

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