TGF-beta1 levels in pre-treatment plasma identify breast cancer patients at risk of developing post-radiotherapy fibrosis.

2.50
Hdl Handle:
http://hdl.handle.net/10541/87851
Title:
TGF-beta1 levels in pre-treatment plasma identify breast cancer patients at risk of developing post-radiotherapy fibrosis.
Authors:
Li, Chenggang; Wilson, Philip B; Levine, Edward; Barber, Jim; Stewart, Alan L; Kumar, Shant
Abstract:
A serious complication of radiotherapy in the treatment of cancer patients is the late onset of fibrosis in normal tissues. Transforming growth factor beta (TGF-beta) is emerging as a key mediator of the fibrotic process through its effects on stimulation of fibroblast proliferation, migration and extracellular matrix (ECM) synthesis. The fact that radiation-induced vascular injury tends to precede the development of fibrosis has led to the suggestion that vascular damage is crucial in its pathogenesis. CD105, the specific type III vascular receptor for TGF-beta1 and -beta3, modulates cell proliferation and ECM production in response to TGF-beta in vitro. In this study, we have quantified the levels of TGF-beta1 and soluble CD105-TGF-beta1 complex in 91 pre-radiotherapy plasma samples from early-stage (T1 or T2) breast cancer patients utilising an enhanced chemiluminescence ELISA system. During the follow-up period, 24 patients had developed moderate and one severe fibrosis of the breast. The mean TGF-beta1 level in these 25 patients was 203.2 +/- 37.3 pg/ml, which was significantly elevated above the level for those with no fibrosis. Furthermore, a significantly lower CD105-TGF-beta1 complex level was observed in the former compared to the latter. Spearman's correlation analysis showed that TGF-beta1 was positively correlated and the CD1O5-TGF-beta1 complex inversely correlated with the occurrence of breast fibrosis. Using a cut-off value of 96 pg/ml, the sensitivity and specificity of TGF-beta1 levels in predicting breast fibrosis were 76% and 74%, respectively. Our results indicate that TGF-beta1 and the receptor-ligand complex appear to be of clinical value in identifying patients at risk of developing post-radiotherapy fibrosis.
Affiliation:
Department of Pathological Sciences, Medical School, University of Manchester, UK.
Citation:
TGF-beta1 levels in pre-treatment plasma identify breast cancer patients at risk of developing post-radiotherapy fibrosis. 1999, 84 (2):155-9 Int. J. Cancer
Journal:
International Journal of Cancer.
Issue Date:
20-Apr-1999
URI:
http://hdl.handle.net/10541/87851
PubMed ID:
10096248
Type:
Article
Language:
en
ISSN:
0020-7136
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Chenggangen
dc.contributor.authorWilson, Philip Ben
dc.contributor.authorLevine, Edwarden
dc.contributor.authorBarber, Jimen
dc.contributor.authorStewart, Alan Len
dc.contributor.authorKumar, Shanten
dc.date.accessioned2009-12-14T13:14:51Z-
dc.date.available2009-12-14T13:14:51Z-
dc.date.issued1999-04-20-
dc.identifier.citationTGF-beta1 levels in pre-treatment plasma identify breast cancer patients at risk of developing post-radiotherapy fibrosis. 1999, 84 (2):155-9 Int. J. Canceren
dc.identifier.issn0020-7136-
dc.identifier.pmid10096248-
dc.identifier.urihttp://hdl.handle.net/10541/87851-
dc.description.abstractA serious complication of radiotherapy in the treatment of cancer patients is the late onset of fibrosis in normal tissues. Transforming growth factor beta (TGF-beta) is emerging as a key mediator of the fibrotic process through its effects on stimulation of fibroblast proliferation, migration and extracellular matrix (ECM) synthesis. The fact that radiation-induced vascular injury tends to precede the development of fibrosis has led to the suggestion that vascular damage is crucial in its pathogenesis. CD105, the specific type III vascular receptor for TGF-beta1 and -beta3, modulates cell proliferation and ECM production in response to TGF-beta in vitro. In this study, we have quantified the levels of TGF-beta1 and soluble CD105-TGF-beta1 complex in 91 pre-radiotherapy plasma samples from early-stage (T1 or T2) breast cancer patients utilising an enhanced chemiluminescence ELISA system. During the follow-up period, 24 patients had developed moderate and one severe fibrosis of the breast. The mean TGF-beta1 level in these 25 patients was 203.2 +/- 37.3 pg/ml, which was significantly elevated above the level for those with no fibrosis. Furthermore, a significantly lower CD105-TGF-beta1 complex level was observed in the former compared to the latter. Spearman's correlation analysis showed that TGF-beta1 was positively correlated and the CD1O5-TGF-beta1 complex inversely correlated with the occurrence of breast fibrosis. Using a cut-off value of 96 pg/ml, the sensitivity and specificity of TGF-beta1 levels in predicting breast fibrosis were 76% and 74%, respectively. Our results indicate that TGF-beta1 and the receptor-ligand complex appear to be of clinical value in identifying patients at risk of developing post-radiotherapy fibrosis.en
dc.language.isoenen
dc.subjectBreast Canceren
dc.subject.meshAdult-
dc.subject.meshAntigens, CD-
dc.subject.meshBiological Markers-
dc.subject.meshBreast-
dc.subject.meshBreast Neoplasms-
dc.subject.meshEnzyme-Linked Immunosorbent Assay-
dc.subject.meshFemale-
dc.subject.meshFibrosis-
dc.subject.meshHumans-
dc.subject.meshImmunoblotting-
dc.subject.meshMiddle Aged-
dc.subject.meshReceptors, Cell Surface-
dc.subject.meshReproducibility of Results-
dc.subject.meshSensitivity and Specificity-
dc.subject.meshTransforming Growth Factor beta-
dc.subject.meshVascular Cell Adhesion Molecule-1-
dc.titleTGF-beta1 levels in pre-treatment plasma identify breast cancer patients at risk of developing post-radiotherapy fibrosis.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pathological Sciences, Medical School, University of Manchester, UK.en
dc.identifier.journalInternational Journal of Cancer.en

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.