Mutation of a phosphorylatable residue in Put3p affects the magnitude of rapamycin-induced PUT1 activation in a Gat1p-dependent manner.

2.50
Hdl Handle:
http://hdl.handle.net/10541/87562
Title:
Mutation of a phosphorylatable residue in Put3p affects the magnitude of rapamycin-induced PUT1 activation in a Gat1p-dependent manner.
Authors:
Leverentz, Michael K; Campbell, Robert N; Connolly, Yvonne; Whetton, Anthony D; Reece, Richard J
Abstract:
Saccharomyces cerevisiae can utilize high quality (e.g. glutamine and ammonia) as well as low quality (e.g. gamma-amino butyric acid and proline) nitrogen sources. The transcriptional activator Put3p allows yeast cells to utilize proline as a nitrogen source through expression of the PUT1 and PUT2 genes. Put3p activates high level transcription of these genes by binding proline directly. However, Put3p also responds to other lower quality nitrogen sources. As nitrogen quality decreases, Put3p exhibits an increase in phosphorylation concurrent with an increase in PUT gene expression. The proline-independent activation of the PUT genes requires both Put3p and the positively acting GATA factors, Gln3p and Gat1p. Conversely, the phosphorylation of Put3p is not dependent on GATA factor activity. Here, we find that the mutation of Put3p at amino acid Tyr-788 modulates the proline-independent activation of PUT1 through Gat1p. The phosphorylation of Put3p appears to influence the association of Gat1p, but not Gln3p, to the PUT1 promoter. Combined, our findings suggest that this may represent a mechanism through which yeast cells rapidly adapt to use proline as a nitrogen source under nitrogen limiting conditions.
Affiliation:
Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom.
Citation:
Mutation of a phosphorylatable residue in Put3p affects the magnitude of rapamycin-induced PUT1 activation in a Gat1p-dependent manner. 2009, 284 (36):24115-22 J. Biol. Chem.
Journal:
The Journal of Biological Chemistry
Issue Date:
4-Sep-2009
URI:
http://hdl.handle.net/10541/87562
DOI:
10.1074/jbc.M109.030361
PubMed ID:
19574222
Type:
Article
Language:
en
ISSN:
0021-9258
Appears in Collections:
All Paterson Institute for Cancer Research; Molecular Biology Core Facility

Full metadata record

DC FieldValue Language
dc.contributor.authorLeverentz, Michael Ken
dc.contributor.authorCampbell, Robert Nen
dc.contributor.authorConnolly, Yvonneen
dc.contributor.authorWhetton, Anthony Den
dc.contributor.authorReece, Richard Jen
dc.date.accessioned2009-12-08T12:16:20Z-
dc.date.available2009-12-08T12:16:20Z-
dc.date.issued2009-09-04-
dc.identifier.citationMutation of a phosphorylatable residue in Put3p affects the magnitude of rapamycin-induced PUT1 activation in a Gat1p-dependent manner. 2009, 284 (36):24115-22 J. Biol. Chem.en
dc.identifier.issn0021-9258-
dc.identifier.pmid19574222-
dc.identifier.doi10.1074/jbc.M109.030361-
dc.identifier.urihttp://hdl.handle.net/10541/87562-
dc.description.abstractSaccharomyces cerevisiae can utilize high quality (e.g. glutamine and ammonia) as well as low quality (e.g. gamma-amino butyric acid and proline) nitrogen sources. The transcriptional activator Put3p allows yeast cells to utilize proline as a nitrogen source through expression of the PUT1 and PUT2 genes. Put3p activates high level transcription of these genes by binding proline directly. However, Put3p also responds to other lower quality nitrogen sources. As nitrogen quality decreases, Put3p exhibits an increase in phosphorylation concurrent with an increase in PUT gene expression. The proline-independent activation of the PUT genes requires both Put3p and the positively acting GATA factors, Gln3p and Gat1p. Conversely, the phosphorylation of Put3p is not dependent on GATA factor activity. Here, we find that the mutation of Put3p at amino acid Tyr-788 modulates the proline-independent activation of PUT1 through Gat1p. The phosphorylation of Put3p appears to influence the association of Gat1p, but not Gln3p, to the PUT1 promoter. Combined, our findings suggest that this may represent a mechanism through which yeast cells rapidly adapt to use proline as a nitrogen source under nitrogen limiting conditions.en
dc.language.isoenen
dc.subject.meshAntifungal Agents-
dc.subject.meshGATA Transcription Factors-
dc.subject.meshGene Expression Regulation, Fungal-
dc.subject.meshMutation-
dc.subject.meshPhosphorylation-
dc.subject.meshProline-
dc.subject.meshProline Oxidase-
dc.subject.meshSaccharomyces cerevisiae-
dc.subject.meshSaccharomyces cerevisiae Proteins-
dc.subject.meshSirolimus-
dc.subject.meshTranscription Factors-
dc.subject.meshTranscription, Genetic-
dc.titleMutation of a phosphorylatable residue in Put3p affects the magnitude of rapamycin-induced PUT1 activation in a Gat1p-dependent manner.en
dc.typeArticleen
dc.contributor.departmentFaculty of Life Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom.en
dc.identifier.journalThe Journal of Biological Chemistryen
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