The transcription factor Atf1 binds and activates the APC/C ubiquitin ligase in fission yeast.

2.50
Hdl Handle:
http://hdl.handle.net/10541/87561
Title:
The transcription factor Atf1 binds and activates the APC/C ubiquitin ligase in fission yeast.
Authors:
Ors, Aslihan; Grimaldi, Margaret; Kimata, Yuu; Wilkinson, Caroline R M; Jones, Nic; Yamano, Hiroyuki
Abstract:
Fission yeast Atf1 is a member of the ATF/CREB basic leucine zipper (bZIP) family of transcription factors with strong homology to mammalian ATF2. Atf1 regulates transcription in response to stress stimuli and also plays a role in controlling heterochromatin formation and recombination. However, its DNA binding independent role is poorly studied. Here, we report that Atf1 has a distinct role in regulating the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. We have identified atf1(+) as a dose-dependent suppressor of apc5-1, a mutation causing mitotic arrest. Remarkably, the suppression is not dependent upon the bZIP domain and is therefore independent of the ability of Atf1 to bind DNA. Interestingly, Atf1 physically binds the APC/C in vivo. Furthermore, we show that addition of purified Atf1 proteins into a cell-free system stimulates ubiquitylation of cyclin B and securin by the APC/C. These results reveal a novel role for Atf1 in cell cycle control through protein-protein interaction.
Affiliation:
Cell Cycle Control Laboratory, Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, United Kingdom.
Citation:
The transcription factor Atf1 binds and activates the APC/C ubiquitin ligase in fission yeast. 2009, 284 (36):23989-94 J. Biol. Chem.
Journal:
The Journal of Biological Chemistry
Issue Date:
4-Sep-2009
URI:
http://hdl.handle.net/10541/87561
DOI:
10.1074/jbc.M109.018309
PubMed ID:
19584054
Type:
Article
Language:
en
ISSN:
0021-9258
Appears in Collections:
All Paterson Institute for Cancer Research; Cell Regulation

Full metadata record

DC FieldValue Language
dc.contributor.authorOrs, Aslihanen
dc.contributor.authorGrimaldi, Margareten
dc.contributor.authorKimata, Yuuen
dc.contributor.authorWilkinson, Caroline R Men
dc.contributor.authorJones, Nicen
dc.contributor.authorYamano, Hiroyukien
dc.date.accessioned2009-12-08T12:15:26Z-
dc.date.available2009-12-08T12:15:26Z-
dc.date.issued2009-09-04-
dc.identifier.citationThe transcription factor Atf1 binds and activates the APC/C ubiquitin ligase in fission yeast. 2009, 284 (36):23989-94 J. Biol. Chem.en
dc.identifier.issn0021-9258-
dc.identifier.pmid19584054-
dc.identifier.doi10.1074/jbc.M109.018309-
dc.identifier.urihttp://hdl.handle.net/10541/87561-
dc.description.abstractFission yeast Atf1 is a member of the ATF/CREB basic leucine zipper (bZIP) family of transcription factors with strong homology to mammalian ATF2. Atf1 regulates transcription in response to stress stimuli and also plays a role in controlling heterochromatin formation and recombination. However, its DNA binding independent role is poorly studied. Here, we report that Atf1 has a distinct role in regulating the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. We have identified atf1(+) as a dose-dependent suppressor of apc5-1, a mutation causing mitotic arrest. Remarkably, the suppression is not dependent upon the bZIP domain and is therefore independent of the ability of Atf1 to bind DNA. Interestingly, Atf1 physically binds the APC/C in vivo. Furthermore, we show that addition of purified Atf1 proteins into a cell-free system stimulates ubiquitylation of cyclin B and securin by the APC/C. These results reveal a novel role for Atf1 in cell cycle control through protein-protein interaction.en
dc.language.isoenen
dc.subject.meshActivating Transcription Factor 1-
dc.subject.meshCell-Free System-
dc.subject.meshCyclin B-
dc.subject.meshHeterochromatin-
dc.subject.meshMitosis-
dc.subject.meshPhosphoproteins-
dc.subject.meshRecombination, Genetic-
dc.subject.meshSchizosaccharomyces-
dc.subject.meshSchizosaccharomyces pombe Proteins-
dc.subject.meshUbiquitin-Protein Ligase Complexes-
dc.subject.meshUbiquitin-Protein Ligases-
dc.titleThe transcription factor Atf1 binds and activates the APC/C ubiquitin ligase in fission yeast.en
dc.typeArticleen
dc.contributor.departmentCell Cycle Control Laboratory, Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, United Kingdom.en
dc.identifier.journalThe Journal of Biological Chemistryen
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