CD105 antagonizes the inhibitory signaling of transforming growth factor beta1 on human vascular endothelial cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/86681
Title:
CD105 antagonizes the inhibitory signaling of transforming growth factor beta1 on human vascular endothelial cells.
Authors:
Li, Chenggang; Hampson, Ian N; Hampson, Lynne; Kumar, Patricia; Bernabeu, Carmelo; Kumar, Shant
Abstract:
CD105 (endoglin), a receptor for transforming growth factor beta (TGFbeta), is highly expressed in tissue-cultured, activated endothelial cells in vitro and in tissues undergoing angiogenesis in vivo. The absence of CD105 in knockout mice leads to their death from defective vascular development, but the role of CD105 in the modulation of angiogenesis has not been elucidated. TGFbeta1 is a well-recognized regulator of angiogenesis. Using an antisense approach, we have shown that inhibition of CD105 protein translation in cultured human endothelial cells enhances the ability of TGFbeta1 to suppress growth and migration in these cells. The ability of endothelial cells to form capillary tubes was evaluated by the use of a 3-dimensional collagen matrix system where TGFbeta1 not only reduced the length of capillary-like structures, but also caused massive mortality in CD105-deficient cells compared to control cultures. These results provide direct evidence that CD105 antagonizes the inhibitory effects of TGFbeta1 on human vascular endothelial cells and that normal cellular levels of CD105 are required for the formation of new blood vessels.
Affiliation:
Department of Pathological Sciences, Medical School, University of Manchester, Manchester M13 9PT, U.K.
Citation:
CD105 antagonizes the inhibitory signaling of transforming growth factor beta1 on human vascular endothelial cells. 2000, 14 (1):55-64 FASEB J.
Journal:
The FASEB Journal
Issue Date:
Jan-2000
URI:
http://hdl.handle.net/10541/86681
PubMed ID:
10627280
Type:
Article
Language:
en
ISSN:
0892-6638
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Chenggangen
dc.contributor.authorHampson, Ian Nen
dc.contributor.authorHampson, Lynneen
dc.contributor.authorKumar, Patriciaen
dc.contributor.authorBernabeu, Carmeloen
dc.contributor.authorKumar, Shanten
dc.date.accessioned2009-11-23T11:34:36Z-
dc.date.available2009-11-23T11:34:36Z-
dc.date.issued2000-01-
dc.identifier.citationCD105 antagonizes the inhibitory signaling of transforming growth factor beta1 on human vascular endothelial cells. 2000, 14 (1):55-64 FASEB J.en
dc.identifier.issn0892-6638-
dc.identifier.pmid10627280-
dc.identifier.urihttp://hdl.handle.net/10541/86681-
dc.description.abstractCD105 (endoglin), a receptor for transforming growth factor beta (TGFbeta), is highly expressed in tissue-cultured, activated endothelial cells in vitro and in tissues undergoing angiogenesis in vivo. The absence of CD105 in knockout mice leads to their death from defective vascular development, but the role of CD105 in the modulation of angiogenesis has not been elucidated. TGFbeta1 is a well-recognized regulator of angiogenesis. Using an antisense approach, we have shown that inhibition of CD105 protein translation in cultured human endothelial cells enhances the ability of TGFbeta1 to suppress growth and migration in these cells. The ability of endothelial cells to form capillary tubes was evaluated by the use of a 3-dimensional collagen matrix system where TGFbeta1 not only reduced the length of capillary-like structures, but also caused massive mortality in CD105-deficient cells compared to control cultures. These results provide direct evidence that CD105 antagonizes the inhibitory effects of TGFbeta1 on human vascular endothelial cells and that normal cellular levels of CD105 are required for the formation of new blood vessels.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshAntigens, CD-
dc.subject.meshBase Sequence-
dc.subject.meshCell Death-
dc.subject.meshCell Movement-
dc.subject.meshCells, Cultured-
dc.subject.meshDNA Primers-
dc.subject.meshEndothelium, Vascular-
dc.subject.meshHumans-
dc.subject.meshMice-
dc.subject.meshNeovascularization, Physiologic-
dc.subject.meshOligonucleotides, Antisense-
dc.subject.meshRNA, Messenger-
dc.subject.meshReceptors, Cell Surface-
dc.subject.meshSignal Transduction-
dc.subject.meshTransforming Growth Factor beta-
dc.subject.meshVascular Cell Adhesion Molecule-1-
dc.titleCD105 antagonizes the inhibitory signaling of transforming growth factor beta1 on human vascular endothelial cells.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pathological Sciences, Medical School, University of Manchester, Manchester M13 9PT, U.K.en
dc.identifier.journalThe FASEB Journalen

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