Fluorescent location of malignant cells in fine needle aspirates.

2.50
Hdl Handle:
http://hdl.handle.net/10541/86647
Title:
Fluorescent location of malignant cells in fine needle aspirates.
Authors:
Steven, F S; El-Teraifi, H; Denton, J; Markidou, S G; Delinassios, J G
Abstract:
Malignant cells in fine needle aspirates possess a cell surface protease which can be targeted with fluorescent affinity probes. Cells with active GB exhibit cell surface fluorescence when stained with such affinity probes. The nuclei of all cells on the slides can be counterstained with a nuclear fluorescent stain. Malignant cells are then located by their cell surface fluorescence and their diagnosis confirmed by examining their fluorescent nuclei. Normal cells and benign cells exhibit no cell surface fluorescence and can be ignored. This technique can be used to rapidly select cells of cytological interest in FNA samples obtained routinely and might be adapted for automated screening of FNA.
Affiliation:
Division of Biochemistry, School of Biological Sciences, University of Manchester, Manchester M13 9PT, U.K.
Citation:
Fluorescent location of malignant cells in fine needle aspirates., 20 (6C):4871-6 Anticancer Res.
Journal:
Anticancer Research
Issue Date:
2000
URI:
http://hdl.handle.net/10541/86647
PubMed ID:
11205235
Type:
Article
Language:
en
ISSN:
0250-7005
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorSteven, F Sen
dc.contributor.authorEl-Teraifi, Hen
dc.contributor.authorDenton, Jen
dc.contributor.authorMarkidou, S Gen
dc.contributor.authorDelinassios, J Gen
dc.date.accessioned2009-11-23T10:13:46Z-
dc.date.available2009-11-23T10:13:46Z-
dc.date.issued2000-
dc.identifier.citationFluorescent location of malignant cells in fine needle aspirates., 20 (6C):4871-6 Anticancer Res.en
dc.identifier.issn0250-7005-
dc.identifier.pmid11205235-
dc.identifier.urihttp://hdl.handle.net/10541/86647-
dc.description.abstractMalignant cells in fine needle aspirates possess a cell surface protease which can be targeted with fluorescent affinity probes. Cells with active GB exhibit cell surface fluorescence when stained with such affinity probes. The nuclei of all cells on the slides can be counterstained with a nuclear fluorescent stain. Malignant cells are then located by their cell surface fluorescence and their diagnosis confirmed by examining their fluorescent nuclei. Normal cells and benign cells exhibit no cell surface fluorescence and can be ignored. This technique can be used to rapidly select cells of cytological interest in FNA samples obtained routinely and might be adapted for automated screening of FNA.en
dc.language.isoenen
dc.subjectCanceren
dc.subjectCancer Pathologyen
dc.subject.meshAutomation-
dc.subject.meshBiopsy, Needle-
dc.subject.meshCell Membrane-
dc.subject.meshEndopeptidases-
dc.subject.meshHumans-
dc.subject.meshMicroscopy, Fluorescence-
dc.subject.meshNeoplasms-
dc.titleFluorescent location of malignant cells in fine needle aspirates.en
dc.typeArticleen
dc.contributor.departmentDivision of Biochemistry, School of Biological Sciences, University of Manchester, Manchester M13 9PT, U.K.en
dc.identifier.journalAnticancer Researchen
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