Assembly and preferential localization of Nup116p on the cytoplasmic face of the nuclear pore complex by interaction with Nup82p.

2.50
Hdl Handle:
http://hdl.handle.net/10541/86376
Title:
Assembly and preferential localization of Nup116p on the cytoplasmic face of the nuclear pore complex by interaction with Nup82p.
Authors:
Ho, Alberto K; Shen, Tian X; Ryan, Kathryn J; Kiseleva, Elena; Levy, Marilyn A; Allen, Terence D; Wente, Susan R
Abstract:
The yeast Saccharomyces cerevisiae nucleoporin Nup116p serves as a docking site for both nuclear import and export factors. However, the mechanism for assembling Nup116p into the nuclear pore complex (NPC) has not been resolved. By conducting a two-hybrid screen with the carboxy (C)-terminal Nup116p region as bait, we identified Nup82p. The predicted coiled-coil region of Nup82p was not required for Nup116p interaction, making the binding requirements distinct from those for the Nsp1p-Nup82p-Nup159p subcomplex (N. Belgareh, C. Snay-Hodge, F. Pasteau, S. Dagher, C. N. Cole, and V. Doye, Mol. Biol. Cell 9:3475-3492, 1998). Immunoprecipitation experiments using yeast cell lysates resulted in the coisolation of a Nup116p-Nup82p subcomplex. Although the absence of Nup116p had no effect on the NPC localization of Nup82p, overexpression of C-terminal Nup116p in a nup116 null mutant resulted in Nup82p mislocalization. Moreover, NPC localization of Nup116p was specifically diminished in a nup82-Delta108 mutant after growth at 37 degrees C. Immunoelectron microscopy analysis showed Nup116p was localized on both the cytoplasmic and nuclear NPC faces. Its distribution was asymmetric with the majority at the cytoplasmic face. Taken together, these results suggest that Nup82p and Nup116p interact at the cytoplasmic NPC face, with nucleoplasmic Nup116p localization utilizing novel binding partners.
Affiliation:
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Citation:
Assembly and preferential localization of Nup116p on the cytoplasmic face of the nuclear pore complex by interaction with Nup82p. 2000, 20 (15):5736-48 Mol. Cell. Biol.
Journal:
Molecular and Cellular Biology
Issue Date:
Aug-2000
URI:
http://hdl.handle.net/10541/86376
PubMed ID:
10891509
Type:
Article
Language:
en
ISSN:
0270-7306
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHo, Alberto Ken
dc.contributor.authorShen, Tian Xen
dc.contributor.authorRyan, Kathryn Jen
dc.contributor.authorKiseleva, Elenaen
dc.contributor.authorLevy, Marilyn Aen
dc.contributor.authorAllen, Terence Den
dc.contributor.authorWente, Susan Ren
dc.date.accessioned2009-11-18T10:33:33Z-
dc.date.available2009-11-18T10:33:33Z-
dc.date.issued2000-08-
dc.identifier.citationAssembly and preferential localization of Nup116p on the cytoplasmic face of the nuclear pore complex by interaction with Nup82p. 2000, 20 (15):5736-48 Mol. Cell. Biol.en
dc.identifier.issn0270-7306-
dc.identifier.pmid10891509-
dc.identifier.urihttp://hdl.handle.net/10541/86376-
dc.description.abstractThe yeast Saccharomyces cerevisiae nucleoporin Nup116p serves as a docking site for both nuclear import and export factors. However, the mechanism for assembling Nup116p into the nuclear pore complex (NPC) has not been resolved. By conducting a two-hybrid screen with the carboxy (C)-terminal Nup116p region as bait, we identified Nup82p. The predicted coiled-coil region of Nup82p was not required for Nup116p interaction, making the binding requirements distinct from those for the Nsp1p-Nup82p-Nup159p subcomplex (N. Belgareh, C. Snay-Hodge, F. Pasteau, S. Dagher, C. N. Cole, and V. Doye, Mol. Biol. Cell 9:3475-3492, 1998). Immunoprecipitation experiments using yeast cell lysates resulted in the coisolation of a Nup116p-Nup82p subcomplex. Although the absence of Nup116p had no effect on the NPC localization of Nup82p, overexpression of C-terminal Nup116p in a nup116 null mutant resulted in Nup82p mislocalization. Moreover, NPC localization of Nup116p was specifically diminished in a nup82-Delta108 mutant after growth at 37 degrees C. Immunoelectron microscopy analysis showed Nup116p was localized on both the cytoplasmic and nuclear NPC faces. Its distribution was asymmetric with the majority at the cytoplasmic face. Taken together, these results suggest that Nup82p and Nup116p interact at the cytoplasmic NPC face, with nucleoplasmic Nup116p localization utilizing novel binding partners.en
dc.language.isoenen
dc.subject.meshCell Nucleus-
dc.subject.meshCytoplasm-
dc.subject.meshFluorescent Antibody Technique-
dc.subject.meshFungal Proteins-
dc.subject.meshGreen Fluorescent Proteins-
dc.subject.meshLuminescent Proteins-
dc.subject.meshMembrane Proteins-
dc.subject.meshMutation-
dc.subject.meshNuclear Pore Complex Proteins-
dc.subject.meshNuclear Proteins-
dc.subject.meshRecombinant Proteins-
dc.subject.meshSaccharomyces cerevisiae-
dc.subject.meshSaccharomyces cerevisiae Proteins-
dc.subject.meshTemperature-
dc.subject.meshTwo-Hybrid System Techniques-
dc.titleAssembly and preferential localization of Nup116p on the cytoplasmic face of the nuclear pore complex by interaction with Nup82p.en
dc.typeArticleen
dc.contributor.departmentDepartment of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.en
dc.identifier.journalMolecular and Cellular Biologyen

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