Topical treatment with liposomes containing T4 endonuclease V protects human skin in vivo from ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha.

2.50
Hdl Handle:
http://hdl.handle.net/10541/86375
Title:
Topical treatment with liposomes containing T4 endonuclease V protects human skin in vivo from ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha.
Authors:
Wolf, Peter; Maier, Harald; Müllegger, Robert R; Chadwick, Caroline A; Hofmann-Wellenhof, Rainer; Soyer, H Peter; Hofer, Angelika; Smolle, Josef; Horn, Michael; Cerroni, Lorenzo; Yarosh, Daniel; Klein, Jonathan; Bucana, Corazon; Dunner, Kenneth; Potten, Christopher S; Hönigsmann, Herbert; Kerl, Helmut; Kripke, Margaret L
Abstract:
Exposing human skin to ultraviolet radiation causes DNA damage, sunburn, immune alterations, and eventually, skin cancer. We wished to determine whether liposomes containing a DNA repair enzyme could prevent any of the acute effects of irradiation when applied after ultraviolet exposure. Fifteen human patients with a prior history of skin cancer were exposed to two minimal erythema doses of ultraviolet radiation on their buttock skin. Liposomes containing T4 endonuclease V or heat-inactivated enzyme were applied immediately and at 2, 4, and 5 h after ultraviolet irradiation. Transmission electron microscopy after anti-T4 endonuclease V-staining and immunogold labeling on biopsies taken at 6 h after ultraviolet exposure revealed that the enzyme was present within cells in the skin. Immunohistochemical DNA damage studies suggested a trend toward improved DNA repair at the active T4 endonuclease V liposome-treated test sites. Although the active T4 endonuclease V liposomes did not significantly affect the ultraviolet-induced erythema response and microscopic sunburn cell formation, they nearly completely prevented ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha RNA message and of interleukin-10 protein. These studies demonstrate that liposomes can be used for topical intracellular delivery of small proteins to human skin and suggest that liposomes containing DNA repair enzymes may provide a new avenue for photoprotection against some forms of ultraviolet-induced skin damage.
Affiliation:
Department of Dermatology, Karl Franzens University, Graz, Austria. peter.wolf@kfunigraz.ac.at
Citation:
Topical treatment with liposomes containing T4 endonuclease V protects human skin in vivo from ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha. 2000, 114 (1):149-56 J. Invest. Dermatol.
Journal:
The Journal of Investigative Dermatology
Issue Date:
Jan-2000
URI:
http://hdl.handle.net/10541/86375
DOI:
10.1046/j.1523-1747.2000.00839.x
PubMed ID:
10620131
Type:
Article
Language:
en
ISSN:
0022-202X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWolf, Peteren
dc.contributor.authorMaier, Haralden
dc.contributor.authorMüllegger, Robert Ren
dc.contributor.authorChadwick, Caroline Aen
dc.contributor.authorHofmann-Wellenhof, Raineren
dc.contributor.authorSoyer, H Peteren
dc.contributor.authorHofer, Angelikaen
dc.contributor.authorSmolle, Josefen
dc.contributor.authorHorn, Michaelen
dc.contributor.authorCerroni, Lorenzoen
dc.contributor.authorYarosh, Danielen
dc.contributor.authorKlein, Jonathanen
dc.contributor.authorBucana, Corazonen
dc.contributor.authorDunner, Kennethen
dc.contributor.authorPotten, Christopher Sen
dc.contributor.authorHönigsmann, Herberten
dc.contributor.authorKerl, Helmuten
dc.contributor.authorKripke, Margaret Len
dc.date.accessioned2009-11-18T10:32:39Z-
dc.date.available2009-11-18T10:32:39Z-
dc.date.issued2000-01-
dc.identifier.citationTopical treatment with liposomes containing T4 endonuclease V protects human skin in vivo from ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha. 2000, 114 (1):149-56 J. Invest. Dermatol.en
dc.identifier.issn0022-202X-
dc.identifier.pmid10620131-
dc.identifier.doi10.1046/j.1523-1747.2000.00839.x-
dc.identifier.urihttp://hdl.handle.net/10541/86375-
dc.description.abstractExposing human skin to ultraviolet radiation causes DNA damage, sunburn, immune alterations, and eventually, skin cancer. We wished to determine whether liposomes containing a DNA repair enzyme could prevent any of the acute effects of irradiation when applied after ultraviolet exposure. Fifteen human patients with a prior history of skin cancer were exposed to two minimal erythema doses of ultraviolet radiation on their buttock skin. Liposomes containing T4 endonuclease V or heat-inactivated enzyme were applied immediately and at 2, 4, and 5 h after ultraviolet irradiation. Transmission electron microscopy after anti-T4 endonuclease V-staining and immunogold labeling on biopsies taken at 6 h after ultraviolet exposure revealed that the enzyme was present within cells in the skin. Immunohistochemical DNA damage studies suggested a trend toward improved DNA repair at the active T4 endonuclease V liposome-treated test sites. Although the active T4 endonuclease V liposomes did not significantly affect the ultraviolet-induced erythema response and microscopic sunburn cell formation, they nearly completely prevented ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha RNA message and of interleukin-10 protein. These studies demonstrate that liposomes can be used for topical intracellular delivery of small proteins to human skin and suggest that liposomes containing DNA repair enzymes may provide a new avenue for photoprotection against some forms of ultraviolet-induced skin damage.en
dc.language.isoenen
dc.subjectTumour Necrosis Factor-alphaen
dc.subject.meshAdministration, Topical-
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshDNA Ligases-
dc.subject.meshDNA Repair-
dc.subject.meshDeoxyribonuclease (Pyrimidine Dimer)-
dc.subject.meshDrug Carriers-
dc.subject.meshEndodeoxyribonucleases-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshInterleukin-10-
dc.subject.meshKeratinocytes-
dc.subject.meshLangerhans Cells-
dc.subject.meshLiposomes-
dc.subject.meshMale-
dc.subject.meshMicroscopy, Electron-
dc.subject.meshMiddle Aged-
dc.subject.meshRadiation-Protective Agents-
dc.subject.meshSkin-
dc.subject.meshTime Factors-
dc.subject.meshTumor Necrosis Factor-alpha-
dc.subject.meshUltraviolet Rays-
dc.subject.meshUp-Regulation-
dc.subject.meshViral Proteins-
dc.titleTopical treatment with liposomes containing T4 endonuclease V protects human skin in vivo from ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha.en
dc.typeArticleen
dc.contributor.departmentDepartment of Dermatology, Karl Franzens University, Graz, Austria. peter.wolf@kfunigraz.ac.aten
dc.identifier.journalThe Journal of Investigative Dermatologyen
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