Giant-cell fibroblastoma: a case report emphasising the presence of hyperplastic subplasmalemmal linear densities in continuity with granular matrices in the extracellular space.

2.50
Hdl Handle:
http://hdl.handle.net/10541/86362
Title:
Giant-cell fibroblastoma: a case report emphasising the presence of hyperplastic subplasmalemmal linear densities in continuity with granular matrices in the extracellular space.
Authors:
Eyden, Brian P; Yamazaki, Kazuto; Banerjee, Saumitra S
Abstract:
The histological, immunohistochemical and ultrastructural features of a case of giant-cell fibroblastoma from the soft tissues of the chest wall in a 48-year-old female are described with special reference to the cell surface and matrix. Subplasmalemmal linear densities (SLDs) characterised cell surfaces, and exhibited excessive development of the dense external component: foci of identical dense material were present in the matrix. The nature of these dense foci, both the external component of the SLD and those free in the extracellular space, was investigated by light microscope immunostaining for fibronectin, laminin and collagen IV. All three proteins stained vessels. There was weaker but positive staining for tumour cell surfaces and matrix, consistent with the widely dispersed nature of the dense foci. Given their fine structural appearance, these dense foci can be referred to as granular matrices. Given also that the matrix protein immunostaining pattern is consistent with the distribution of these granular matrices as observed by electron microscopy, they may be provisionally interpreted as a kind of basement-membrane-related granular matrix. The presence of these proteins emphasises the point that, while giant-cell fibroblastoma fibroblasts lack a lamina, they nevertheless bear basement-membrane-related proteins organised, however, in a non-laminate fashion. The observations reinforce the need to qualify immunostaining results by ultrastructural investigation in order to understand the organisation of immuno-detected proteins and are discussed in terms of their diagnostic and possible biological significance.
Affiliation:
Department of Histopathology, Christie Hospital NHS Trust, Manchester, UK. Brian.Eyden@christie-tr.nwest.nhs.uk
Citation:
Giant-cell fibroblastoma: a case report emphasising the presence of hyperplastic subplasmalemmal linear densities in continuity with granular matrices in the extracellular space. 2000, 32 (4):509-14 J. Submicrosc. Cytol. Pathol.
Journal:
Journal of Submicroscopic Cytology and Pathology
Issue Date:
Oct-2000
URI:
http://hdl.handle.net/10541/86362
PubMed ID:
11297369
Type:
Article
Language:
en
ISSN:
1122-9497
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorEyden, Brian Pen
dc.contributor.authorYamazaki, Kazutoen
dc.contributor.authorBanerjee, Saumitra Sen
dc.date.accessioned2009-11-18T10:48:05Z-
dc.date.available2009-11-18T10:48:05Z-
dc.date.issued2000-10-
dc.identifier.citationGiant-cell fibroblastoma: a case report emphasising the presence of hyperplastic subplasmalemmal linear densities in continuity with granular matrices in the extracellular space. 2000, 32 (4):509-14 J. Submicrosc. Cytol. Pathol.en
dc.identifier.issn1122-9497-
dc.identifier.pmid11297369-
dc.identifier.urihttp://hdl.handle.net/10541/86362-
dc.description.abstractThe histological, immunohistochemical and ultrastructural features of a case of giant-cell fibroblastoma from the soft tissues of the chest wall in a 48-year-old female are described with special reference to the cell surface and matrix. Subplasmalemmal linear densities (SLDs) characterised cell surfaces, and exhibited excessive development of the dense external component: foci of identical dense material were present in the matrix. The nature of these dense foci, both the external component of the SLD and those free in the extracellular space, was investigated by light microscope immunostaining for fibronectin, laminin and collagen IV. All three proteins stained vessels. There was weaker but positive staining for tumour cell surfaces and matrix, consistent with the widely dispersed nature of the dense foci. Given their fine structural appearance, these dense foci can be referred to as granular matrices. Given also that the matrix protein immunostaining pattern is consistent with the distribution of these granular matrices as observed by electron microscopy, they may be provisionally interpreted as a kind of basement-membrane-related granular matrix. The presence of these proteins emphasises the point that, while giant-cell fibroblastoma fibroblasts lack a lamina, they nevertheless bear basement-membrane-related proteins organised, however, in a non-laminate fashion. The observations reinforce the need to qualify immunostaining results by ultrastructural investigation in order to understand the organisation of immuno-detected proteins and are discussed in terms of their diagnostic and possible biological significance.en
dc.language.isoenen
dc.subjectGiant Cell Tumoursen
dc.subjectSoft Tissue Canceren
dc.subject.meshBasement Membrane-
dc.subject.meshCell Membrane-
dc.subject.meshExtracellular Matrix-
dc.subject.meshFemale-
dc.subject.meshFibroma-
dc.subject.meshGiant Cell Tumors-
dc.subject.meshHumans-
dc.subject.meshHyperplasia-
dc.subject.meshImmunohistochemistry-
dc.subject.meshMembrane Proteins-
dc.subject.meshMicroscopy, Electron-
dc.subject.meshMiddle Aged-
dc.subject.meshSoft Tissue Neoplasms-
dc.titleGiant-cell fibroblastoma: a case report emphasising the presence of hyperplastic subplasmalemmal linear densities in continuity with granular matrices in the extracellular space.en
dc.typeArticleen
dc.contributor.departmentDepartment of Histopathology, Christie Hospital NHS Trust, Manchester, UK. Brian.Eyden@christie-tr.nwest.nhs.uken
dc.identifier.journalJournal of Submicroscopic Cytology and Pathologyen

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