Human papillomavirus type 16 E6/E7-specific cytotoxic T lymphocytes in women with cervical neoplasia.

2.50
Hdl Handle:
http://hdl.handle.net/10541/86357
Title:
Human papillomavirus type 16 E6/E7-specific cytotoxic T lymphocytes in women with cervical neoplasia.
Authors:
Bontkes, Hetty J; De Gruijl, Tanja D; Van den Muysenberg, Adrie J; Verheijen, René H; Stukart, Marij J; Meijer, Chris J L M; Scheper, Rik J; Stacey, Simon N; Duggan-Keen, Margaret F; Stern, Peter L; Man, Stephen; Borysiewicz, Leszek K; Walboomers, Jan M
Abstract:
Infection with oncogenic human papillomavirus (HPV) types is associated with the development of cervical neoplasia (CIN). The E6 and E7 oncoproteins are constitutively expressed in these lesions and are therefore putative targets for the immune response against HPV. The relation between HPV 16-specific memory cytotoxic T-cell precursor (mCTLp) activity to both oncoproteins and the natural course of cervical dysplasia was analyzed in 38 patients participating in a nonintervention cohort study of women with CIN and 11 HPV 16-positive cervical carcinoma patients. In a cross-sectional study at the end of follow-up prior to biopsy, 8 of 20 patients with a persistent HPV 16 infection had specific mCTLp against at least one of the two oncoproteins. By contrast, no specific mCTLp activity was detected in 11 HPV-negative patients or in 7 patients who had cleared an HPV 16 infection at the end of follow-up. However, 5 of 11 cervical carcinoma patients showed mCTLp activity against the E7 protein only. This study demonstrates that HPV 16 oncogene-specific mCTLp are present in women with HPV 16-positive CIN prior to any intervention. Since HPV-specific mCTLp were detected predominantly in women with high-grade lesions or invasive cervical carcinoma and not in women who cleared the virus, the role of naturally occurring mCTLp in the protection against HPV-associated cervical neoplasia remains to be established.
Affiliation:
Department of Pathology, Academisch Ziekenhuis Vrije Universiteit, Amsterdam, the Netherlands.
Citation:
Human papillomavirus type 16 E6/E7-specific cytotoxic T lymphocytes in women with cervical neoplasia. 2000, 88 (1):92-8 Int. J. Cancer
Journal:
International Journal of Cancer.
Issue Date:
1-Oct-2000
URI:
http://hdl.handle.net/10541/86357
PubMed ID:
10962445
Type:
Article
Language:
en
ISSN:
0020-7136
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBontkes, Hetty Jen
dc.contributor.authorDe Gruijl, Tanja Den
dc.contributor.authorVan den Muysenberg, Adrie Jen
dc.contributor.authorVerheijen, René Hen
dc.contributor.authorStukart, Marij Jen
dc.contributor.authorMeijer, Chris J L Men
dc.contributor.authorScheper, Rik Jen
dc.contributor.authorStacey, Simon Nen
dc.contributor.authorDuggan-Keen, Margaret Fen
dc.contributor.authorStern, Peter Len
dc.contributor.authorMan, Stephenen
dc.contributor.authorBorysiewicz, Leszek Ken
dc.contributor.authorWalboomers, Jan Men
dc.date.accessioned2009-11-18T09:55:40Z-
dc.date.available2009-11-18T09:55:40Z-
dc.date.issued2000-10-01-
dc.identifier.citationHuman papillomavirus type 16 E6/E7-specific cytotoxic T lymphocytes in women with cervical neoplasia. 2000, 88 (1):92-8 Int. J. Canceren
dc.identifier.issn0020-7136-
dc.identifier.pmid10962445-
dc.identifier.urihttp://hdl.handle.net/10541/86357-
dc.description.abstractInfection with oncogenic human papillomavirus (HPV) types is associated with the development of cervical neoplasia (CIN). The E6 and E7 oncoproteins are constitutively expressed in these lesions and are therefore putative targets for the immune response against HPV. The relation between HPV 16-specific memory cytotoxic T-cell precursor (mCTLp) activity to both oncoproteins and the natural course of cervical dysplasia was analyzed in 38 patients participating in a nonintervention cohort study of women with CIN and 11 HPV 16-positive cervical carcinoma patients. In a cross-sectional study at the end of follow-up prior to biopsy, 8 of 20 patients with a persistent HPV 16 infection had specific mCTLp against at least one of the two oncoproteins. By contrast, no specific mCTLp activity was detected in 11 HPV-negative patients or in 7 patients who had cleared an HPV 16 infection at the end of follow-up. However, 5 of 11 cervical carcinoma patients showed mCTLp activity against the E7 protein only. This study demonstrates that HPV 16 oncogene-specific mCTLp are present in women with HPV 16-positive CIN prior to any intervention. Since HPV-specific mCTLp were detected predominantly in women with high-grade lesions or invasive cervical carcinoma and not in women who cleared the virus, the role of naturally occurring mCTLp in the protection against HPV-associated cervical neoplasia remains to be established.en
dc.language.isoenen
dc.subjectTumour Virus Infectionsen
dc.subjectUterine Cervical Canceren
dc.subject.meshCervical Intraepithelial Neoplasia-
dc.subject.meshCohort Studies-
dc.subject.meshCross-Sectional Studies-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshImmunologic Memory-
dc.subject.meshInterleukin-2-
dc.subject.meshLymphocyte Activation-
dc.subject.meshMiddle Aged-
dc.subject.meshOncogene Proteins, Viral-
dc.subject.meshPapillomaviridae-
dc.subject.meshPapillomavirus Infections-
dc.subject.meshRepressor Proteins-
dc.subject.meshT-Lymphocytes, Cytotoxic-
dc.subject.meshT-Lymphocytes, Helper-Inducer-
dc.subject.meshTumor Virus Infections-
dc.subject.meshUterine Cervical Neoplasms-
dc.titleHuman papillomavirus type 16 E6/E7-specific cytotoxic T lymphocytes in women with cervical neoplasia.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pathology, Academisch Ziekenhuis Vrije Universiteit, Amsterdam, the Netherlands.en
dc.identifier.journalInternational Journal of Cancer.en

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