The nuclear pore complex: mediator of translocation between nucleus and cytoplasm.

2.50
Hdl Handle:
http://hdl.handle.net/10541/86188
Title:
The nuclear pore complex: mediator of translocation between nucleus and cytoplasm.
Authors:
Allen, Terence D; Cronshaw, J M; Bagley, Steven; Kiseleva, Elena; Goldberg, Martin W
Abstract:
The enclosure of nuclear contents in eukaryotes means that cells require sites in the boundary that mediate exchange of material between nucleus and cytoplasm. These sites, termed nuclear pore complexes (NPCs), number 100-200 in yeast, a few thousand in mammalian cells and approximately 50 million in the giant nuclei of amphibian oocytes. NPCs are large (125 MDa) macromolecular complexes that comprise 50-100 different proteins in vertebrates. In spite of their size and complex structure, NPCs undergo complete breakdown and reformation at cell division. Transport through NPCs can be rapid (estimated at several hundred molecules/pore/second) and accommodates both passive diffusion of relatively small molecules, and active transport of complexes up to several megadaltons in molecular mass. Each pore can facilitate both import and export. The two processes apparently involve multiple pathways for different cargoes, and their transport signals, transport receptors and adapters, and the molecules (and their regulators) that underpin the transport mechanisms. Over the past few years there has been an increasing interest in the pore complex: structural studies have been followed by elucidation of the biochemical aspects of nuclear import, and subsequent investigations into nuclear export. The current challenge is to understand the interactions between the structural elements of the pore complex and the mechanisms that drive the physical processes of translocation through it.
Affiliation:
CRC Structural Cell Biology Group, Paterson Institute, Christie Hospital, Manchester, M20 4BX, UK.
Citation:
The nuclear pore complex: mediator of translocation between nucleus and cytoplasm. 2000, 113 ( Pt 10):1651-9 J. Cell. Sci.
Journal:
Journal of Cell Science
Issue Date:
May-2000
URI:
http://hdl.handle.net/10541/86188
PubMed ID:
10769196
Type:
Article
Language:
en
ISSN:
0021-9533
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorAllen, Terence Den
dc.contributor.authorCronshaw, J Men
dc.contributor.authorBagley, Stevenen
dc.contributor.authorKiseleva, Elenaen
dc.contributor.authorGoldberg, Martin Wen
dc.date.accessioned2009-11-13T15:23:53Z-
dc.date.available2009-11-13T15:23:53Z-
dc.date.issued2000-05-
dc.identifier.citationThe nuclear pore complex: mediator of translocation between nucleus and cytoplasm. 2000, 113 ( Pt 10):1651-9 J. Cell. Sci.en
dc.identifier.issn0021-9533-
dc.identifier.pmid10769196-
dc.identifier.urihttp://hdl.handle.net/10541/86188-
dc.description.abstractThe enclosure of nuclear contents in eukaryotes means that cells require sites in the boundary that mediate exchange of material between nucleus and cytoplasm. These sites, termed nuclear pore complexes (NPCs), number 100-200 in yeast, a few thousand in mammalian cells and approximately 50 million in the giant nuclei of amphibian oocytes. NPCs are large (125 MDa) macromolecular complexes that comprise 50-100 different proteins in vertebrates. In spite of their size and complex structure, NPCs undergo complete breakdown and reformation at cell division. Transport through NPCs can be rapid (estimated at several hundred molecules/pore/second) and accommodates both passive diffusion of relatively small molecules, and active transport of complexes up to several megadaltons in molecular mass. Each pore can facilitate both import and export. The two processes apparently involve multiple pathways for different cargoes, and their transport signals, transport receptors and adapters, and the molecules (and their regulators) that underpin the transport mechanisms. Over the past few years there has been an increasing interest in the pore complex: structural studies have been followed by elucidation of the biochemical aspects of nuclear import, and subsequent investigations into nuclear export. The current challenge is to understand the interactions between the structural elements of the pore complex and the mechanisms that drive the physical processes of translocation through it.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBiological Transport-
dc.subject.meshCytoplasm-
dc.subject.meshHumans-
dc.subject.meshNuclear Envelope-
dc.titleThe nuclear pore complex: mediator of translocation between nucleus and cytoplasm.en
dc.typeArticleen
dc.contributor.departmentCRC Structural Cell Biology Group, Paterson Institute, Christie Hospital, Manchester, M20 4BX, UK.en
dc.identifier.journalJournal of Cell Scienceen

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