Protection by ultraviolet A and B sunscreens against in situ dipyrimidine photolesions in human epidermis is comparable to protection against sunburn.

2.50
Hdl Handle:
http://hdl.handle.net/10541/86186
Title:
Protection by ultraviolet A and B sunscreens against in situ dipyrimidine photolesions in human epidermis is comparable to protection against sunburn.
Authors:
Young, Antony R; Sheehan, John M; Chadwick, Caroline A; Potten, Christopher S
Abstract:
Sunscreens prevent sunburn and may also prevent skin cancer by protecting from ultraviolet-induced DNA damage. We assessed the ability of two sunscreens, with different spectral profiles, to inhibit DNA photodamage in human epidermis in situ. One formulation contained the established ultraviolet B filter octyl methoxycinnamate, whereas the other contained terephthalylidene dicamphor sulfonic acid, a new ultraviolet A filter. Both formulations had sun protection factors of 4 when assessed with solar simulating radiation in volunteers of skin type I/II. We tested the hypothesis that sun protection factors would indicate the level of protection against DNA photodamage. Thus, we exposed sunscreen-treated sites to four times the minimal erythema dose of solar simulating radiation, whereas vehicle and control sites were exposed to one minimal erythema dose. We used monoclonal antibodies against thymine dimers and 6-4 photoproducts and image analysis to quantify DNA damage in skin sections. A dose of four times the minimal erythema dose, with either sunscreen, resulted in comparable levels of thymine dimers and 6-4 photoproducts to one minimal erythema dose +/- vehicle, providing evidence that the DNA protection factor is comparable to the sun protection factor. The lack of difference between the sunscreens indicates similar action spectra for erythema and DNA photodamage and that erythema is a clinical surrogate for DNA photodamage that may lead to skin cancer.
Affiliation:
Department of Environmental Dermatology, St. John's Institute of Dermatology, King's College London, St. Thomas's Hospital, London, UK. antony.r.young@kcl.ac.uk
Citation:
Protection by ultraviolet A and B sunscreens against in situ dipyrimidine photolesions in human epidermis is comparable to protection against sunburn. 2000, 115 (1):37-41 J. Invest. Dermatol.
Journal:
The Journal of Investigative Dermatology
Issue Date:
Jul-2000
URI:
http://hdl.handle.net/10541/86186
DOI:
10.1046/j.1523-1747.2000.00012.x
PubMed ID:
10886505
Type:
Article
Language:
en
ISSN:
0022-202X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorYoung, Antony Ren
dc.contributor.authorSheehan, John Men
dc.contributor.authorChadwick, Caroline Aen
dc.contributor.authorPotten, Christopher Sen
dc.date.accessioned2009-11-13T15:19:20Z-
dc.date.available2009-11-13T15:19:20Z-
dc.date.issued2000-07-
dc.identifier.citationProtection by ultraviolet A and B sunscreens against in situ dipyrimidine photolesions in human epidermis is comparable to protection against sunburn. 2000, 115 (1):37-41 J. Invest. Dermatol.en
dc.identifier.issn0022-202X-
dc.identifier.pmid10886505-
dc.identifier.doi10.1046/j.1523-1747.2000.00012.x-
dc.identifier.urihttp://hdl.handle.net/10541/86186-
dc.description.abstractSunscreens prevent sunburn and may also prevent skin cancer by protecting from ultraviolet-induced DNA damage. We assessed the ability of two sunscreens, with different spectral profiles, to inhibit DNA photodamage in human epidermis in situ. One formulation contained the established ultraviolet B filter octyl methoxycinnamate, whereas the other contained terephthalylidene dicamphor sulfonic acid, a new ultraviolet A filter. Both formulations had sun protection factors of 4 when assessed with solar simulating radiation in volunteers of skin type I/II. We tested the hypothesis that sun protection factors would indicate the level of protection against DNA photodamage. Thus, we exposed sunscreen-treated sites to four times the minimal erythema dose of solar simulating radiation, whereas vehicle and control sites were exposed to one minimal erythema dose. We used monoclonal antibodies against thymine dimers and 6-4 photoproducts and image analysis to quantify DNA damage in skin sections. A dose of four times the minimal erythema dose, with either sunscreen, resulted in comparable levels of thymine dimers and 6-4 photoproducts to one minimal erythema dose +/- vehicle, providing evidence that the DNA protection factor is comparable to the sun protection factor. The lack of difference between the sunscreens indicates similar action spectra for erythema and DNA photodamage and that erythema is a clinical surrogate for DNA photodamage that may lead to skin cancer.en
dc.language.isoenen
dc.subject.meshAdolescent-
dc.subject.meshAdult-
dc.subject.meshDNA Damage-
dc.subject.meshErythema-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshPyrimidine Dimers-
dc.subject.meshSkin-
dc.subject.meshSkin Aging-
dc.subject.meshSunburn-
dc.subject.meshSunscreening Agents-
dc.subject.meshUltraviolet Rays-
dc.titleProtection by ultraviolet A and B sunscreens against in situ dipyrimidine photolesions in human epidermis is comparable to protection against sunburn.en
dc.typeArticleen
dc.contributor.departmentDepartment of Environmental Dermatology, St. John's Institute of Dermatology, King's College London, St. Thomas's Hospital, London, UK. antony.r.young@kcl.ac.uken
dc.identifier.journalThe Journal of Investigative Dermatologyen
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