2.50
Hdl Handle:
http://hdl.handle.net/10541/86124
Title:
Fern spore extracts can damage DNA.
Authors:
Simán, S E; Povey, Andrew C; Ward, Timothy H; Margison, Geoffrey P; Sheffield, E
Abstract:
The carcinogenicity of the vegetative tissues of bracken fern (Pteridium) has long been established. More recently, the carcinogenic effects of the spores of bracken have also been recognized. Both vegetative tissues and spores of bracken can induce adducts in DNA in animal tissues, but the possible genotoxic or carcinogenic effects of spores from fern species other than bracken are unknown. The single-cell gel electrophoresis ('comet') assay was used to investigate whether fern spores can cause DNA damage in vitro. Extracts of spores from six fern species were administered to cultured human premyeloid leukaemia (K562) cells. Spore extracts of five fern species: Anemia phyllitidis, Dicksonia antarctica, Pteridium aquilinum, Pteris vittata and Sadleria pallida, induced significantly more DNA strand breaks than those in the control groups. Only in one species, Osmunda regalis, was the effect no different from that in the control groups. Using extracts from A. phyllitidis and P. vittata, the extent of DNA damage was increased by increasing the original dose 10 times, whereas an experiment in which exposure times were varied suggested that the highest levels of strand breaks appear after 2 h exposure. Simultaneous incubation with human S9 liver enzyme mix ablated the damaging effect of the extracts. Our data show that fern spore extracts can cause DNA damage in human cells in vitro. Considering the strong correlation between DNA damage and carcinogenic events, the observations made in this report may well have some implications for human health.
Affiliation:
CRC Carcinogenesis Group, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.
Citation:
Fern spore extracts can damage DNA. 2000, 83 (1):69-73 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
Jul-2000
URI:
http://hdl.handle.net/10541/86124
DOI:
10.1054/bjoc.2000.1204
PubMed ID:
10883670
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorSimán, S Een
dc.contributor.authorPovey, Andrew Cen
dc.contributor.authorWard, Timothy Hen
dc.contributor.authorMargison, Geoffrey Pen
dc.contributor.authorSheffield, Een
dc.date.accessioned2009-11-13T13:13:02Z-
dc.date.available2009-11-13T13:13:02Z-
dc.date.issued2000-07-
dc.identifier.citationFern spore extracts can damage DNA. 2000, 83 (1):69-73 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid10883670-
dc.identifier.doi10.1054/bjoc.2000.1204-
dc.identifier.urihttp://hdl.handle.net/10541/86124-
dc.description.abstractThe carcinogenicity of the vegetative tissues of bracken fern (Pteridium) has long been established. More recently, the carcinogenic effects of the spores of bracken have also been recognized. Both vegetative tissues and spores of bracken can induce adducts in DNA in animal tissues, but the possible genotoxic or carcinogenic effects of spores from fern species other than bracken are unknown. The single-cell gel electrophoresis ('comet') assay was used to investigate whether fern spores can cause DNA damage in vitro. Extracts of spores from six fern species were administered to cultured human premyeloid leukaemia (K562) cells. Spore extracts of five fern species: Anemia phyllitidis, Dicksonia antarctica, Pteridium aquilinum, Pteris vittata and Sadleria pallida, induced significantly more DNA strand breaks than those in the control groups. Only in one species, Osmunda regalis, was the effect no different from that in the control groups. Using extracts from A. phyllitidis and P. vittata, the extent of DNA damage was increased by increasing the original dose 10 times, whereas an experiment in which exposure times were varied suggested that the highest levels of strand breaks appear after 2 h exposure. Simultaneous incubation with human S9 liver enzyme mix ablated the damaging effect of the extracts. Our data show that fern spore extracts can cause DNA damage in human cells in vitro. Considering the strong correlation between DNA damage and carcinogenic events, the observations made in this report may well have some implications for human health.en
dc.language.isoenen
dc.subjectCancer DNAen
dc.subject.meshComet Assay-
dc.subject.meshDNA-
dc.subject.meshDNA Damage-
dc.subject.meshDNA, Neoplasm-
dc.subject.meshHumans-
dc.subject.meshImage Processing, Computer-Assisted-
dc.subject.meshK562 Cells-
dc.subject.meshMetabolic Detoxication, Drug-
dc.subject.meshMicrosomes, Liver-
dc.subject.meshMutagens-
dc.subject.meshPlant Extracts-
dc.subject.meshPlant Physiological Phenomena-
dc.subject.meshSpecies Specificity-
dc.subject.meshSpores-
dc.titleFern spore extracts can damage DNA.en
dc.typeArticleen
dc.contributor.departmentCRC Carcinogenesis Group, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren

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