Study of interlaboratory reliability and reproducibility of estrogen and progesterone receptor assays in Europe. Documentation of poor reliability and identification of insufficient microwave antigen retrieval time as a major contributory element of unreliable assays.

2.50
Hdl Handle:
http://hdl.handle.net/10541/85765
Title:
Study of interlaboratory reliability and reproducibility of estrogen and progesterone receptor assays in Europe. Documentation of poor reliability and identification of insufficient microwave antigen retrieval time as a major contributory element of unreliable assays.
Authors:
Rhodes, Anthony; Jasani, Bharat; Balaton, Andre J; Barnes, Diana M; Anderson, Elizabeth; Bobrow, Lynda G; Miller, Keith D
Abstract:
Immunohistochemical assays for estrogen receptors (ERs) and progesterone receptors (PRs) have not been surveyed for technical validity. In the present study, the reliability of the immunohistochemical assay for ER and PR was evaluated using data from 105 laboratories participating in external quality assessment (EQA) during a 2-year period. Technical variables associated with reliable immunostaining were analyzed. The efficiency of the antigen retrieval step was identified as the single most important contributory factor influencing the overall reproducibility of the assays. Reliable assays were found in 24 (36%) of 66 laboratories participating in continual EQA, including the majority of centers known to have clinically validated results. Inadequate assay sensitivity, with subsequent weak staining, was the main cause of poor and variable results by laboratories using microwave antigen retrieval; too short a heating time was identified as the principal contributory factor. Extension of the heating time resulted in significant improvement regardless of all other variables in the immunohistochemical protocol. Continual participation in EQA is an effective means for identifying and ameliorating variables that influence the reliability of immunohistochemical assays for predictive markers, thereby assisting in technical validation and standardization.
Affiliation:
United Kingdom National External Quality Assessment Scheme for Immunocytochemistry and the Department of Histopathology, University College London Medical School, London, England.
Citation:
Study of interlaboratory reliability and reproducibility of estrogen and progesterone receptor assays in Europe. Documentation of poor reliability and identification of insufficient microwave antigen retrieval time as a major contributory element of unreliable assays. 2001, 115 (1):44-58 Am. J. Clin. Pathol.
Journal:
American Journal of Clinical Pathology
Issue Date:
Jan-2001
URI:
http://hdl.handle.net/10541/85765
DOI:
10.1309/H905-HYC1-6UQQ-981P
PubMed ID:
11190807
Type:
Article
Language:
en
ISSN:
0002-9173
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorRhodes, Anthonyen
dc.contributor.authorJasani, Bharaten
dc.contributor.authorBalaton, Andre Jen
dc.contributor.authorBarnes, Diana Men
dc.contributor.authorAnderson, Elizabethen
dc.contributor.authorBobrow, Lynda Gen
dc.contributor.authorMiller, Keith Den
dc.date.accessioned2009-11-10T10:40:45Z-
dc.date.available2009-11-10T10:40:45Z-
dc.date.issued2001-01-
dc.identifier.citationStudy of interlaboratory reliability and reproducibility of estrogen and progesterone receptor assays in Europe. Documentation of poor reliability and identification of insufficient microwave antigen retrieval time as a major contributory element of unreliable assays. 2001, 115 (1):44-58 Am. J. Clin. Pathol.en
dc.identifier.issn0002-9173-
dc.identifier.pmid11190807-
dc.identifier.doi10.1309/H905-HYC1-6UQQ-981P-
dc.identifier.urihttp://hdl.handle.net/10541/85765-
dc.description.abstractImmunohistochemical assays for estrogen receptors (ERs) and progesterone receptors (PRs) have not been surveyed for technical validity. In the present study, the reliability of the immunohistochemical assay for ER and PR was evaluated using data from 105 laboratories participating in external quality assessment (EQA) during a 2-year period. Technical variables associated with reliable immunostaining were analyzed. The efficiency of the antigen retrieval step was identified as the single most important contributory factor influencing the overall reproducibility of the assays. Reliable assays were found in 24 (36%) of 66 laboratories participating in continual EQA, including the majority of centers known to have clinically validated results. Inadequate assay sensitivity, with subsequent weak staining, was the main cause of poor and variable results by laboratories using microwave antigen retrieval; too short a heating time was identified as the principal contributory factor. Extension of the heating time resulted in significant improvement regardless of all other variables in the immunohistochemical protocol. Continual participation in EQA is an effective means for identifying and ameliorating variables that influence the reliability of immunohistochemical assays for predictive markers, thereby assisting in technical validation and standardization.en
dc.language.isoenen
dc.subjectOestrogen Receptorsen
dc.subject.meshAntigens-
dc.subject.meshEurope-
dc.subject.meshHot Temperature-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshLaboratories-
dc.subject.meshMicrowaves-
dc.subject.meshReceptors, Estrogen-
dc.subject.meshReceptors, Progesterone-
dc.subject.meshReproducibility of Results-
dc.subject.meshTime Factors-
dc.titleStudy of interlaboratory reliability and reproducibility of estrogen and progesterone receptor assays in Europe. Documentation of poor reliability and identification of insufficient microwave antigen retrieval time as a major contributory element of unreliable assays.en
dc.typeArticleen
dc.contributor.departmentUnited Kingdom National External Quality Assessment Scheme for Immunocytochemistry and the Department of Histopathology, University College London Medical School, London, England.en
dc.identifier.journalAmerican Journal of Clinical Pathologyen

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