In vitro labelling studies and the measurement of epithelial cell proliferative activity in the human oral cavity.

2.50
Hdl Handle:
http://hdl.handle.net/10541/85764
Title:
In vitro labelling studies and the measurement of epithelial cell proliferative activity in the human oral cavity.
Authors:
Thomson, P J; Potten, Christopher S; Appleton, D R
Abstract:
Samples (110) of human mandibular gingiva and buccal mucosa, harvested from patients undergoing third-molar surgery, were subjected to in vitro labelling with tritiated thymidine, bromodeoxyuridine, or a sequential double-labelling technique comprising tritiated thymidine followed by bromodeoxyuridine, in order to determine the efficacy of a new incubation labelling technique, and to characterize S-phase labelling indices in human oral mucosa. Whilst, there were no demonstrable differences in labelling indices obtained by single thymidine, single bromodeoxyuridine or double labelling, there was a significant difference between anatomical sites, with higher S phase labelling observed in buccal mucosa (mean LI 11.7) than mandibular gingiva (mean LI 8.5; P<0.01). There was, however, no significant correlation between individual labelling indices and patient age, sex or the time of day when tissue was harvested. The in vitro labelling technique provides a reliable and quantifiable method of characterizing proliferative labelling indices in the human oral cavity. Further investigation is being carried out to profile wider age and anatomical ranges and to utilize the double-labelling technique to calculate S-phase durations and cell-cycle times. These profiles may have a future role in the assessment of oral mucous membrane disease.
Affiliation:
Department of Oral and Maxillofacial Surgery, The Dental School, University of Newcastle, Framlington Place, NE2 4BW, Newcastle upon Tyne, UK. peter.thomson@nd.ac.uk
Citation:
In vitro labelling studies and the measurement of epithelial cell proliferative activity in the human oral cavity. 2001, 46 (12):1157-64 Arch. Oral Biol.
Journal:
Archives of Oral Biology
Issue Date:
Dec-2001
URI:
http://hdl.handle.net/10541/85764
PubMed ID:
11684035
Type:
Article
Language:
en
ISSN:
0003-9969
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorThomson, P Jen
dc.contributor.authorPotten, Christopher Sen
dc.contributor.authorAppleton, D Ren
dc.date.accessioned2009-11-10T10:38:55Z-
dc.date.available2009-11-10T10:38:55Z-
dc.date.issued2001-12-
dc.identifier.citationIn vitro labelling studies and the measurement of epithelial cell proliferative activity in the human oral cavity. 2001, 46 (12):1157-64 Arch. Oral Biol.en
dc.identifier.issn0003-9969-
dc.identifier.pmid11684035-
dc.identifier.urihttp://hdl.handle.net/10541/85764-
dc.description.abstractSamples (110) of human mandibular gingiva and buccal mucosa, harvested from patients undergoing third-molar surgery, were subjected to in vitro labelling with tritiated thymidine, bromodeoxyuridine, or a sequential double-labelling technique comprising tritiated thymidine followed by bromodeoxyuridine, in order to determine the efficacy of a new incubation labelling technique, and to characterize S-phase labelling indices in human oral mucosa. Whilst, there were no demonstrable differences in labelling indices obtained by single thymidine, single bromodeoxyuridine or double labelling, there was a significant difference between anatomical sites, with higher S phase labelling observed in buccal mucosa (mean LI 11.7) than mandibular gingiva (mean LI 8.5; P<0.01). There was, however, no significant correlation between individual labelling indices and patient age, sex or the time of day when tissue was harvested. The in vitro labelling technique provides a reliable and quantifiable method of characterizing proliferative labelling indices in the human oral cavity. Further investigation is being carried out to profile wider age and anatomical ranges and to utilize the double-labelling technique to calculate S-phase durations and cell-cycle times. These profiles may have a future role in the assessment of oral mucous membrane disease.en
dc.language.isoenen
dc.subject.meshAdolescent-
dc.subject.meshAdult-
dc.subject.meshAge Factors-
dc.subject.meshBromodeoxyuridine-
dc.subject.meshCell Division-
dc.subject.meshCells, Cultured-
dc.subject.meshCircadian Rhythm-
dc.subject.meshDNA Replication-
dc.subject.meshFemale-
dc.subject.meshGingiva-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshMitotic Index-
dc.subject.meshMouth Mucosa-
dc.subject.meshS Phase-
dc.subject.meshSex Factors-
dc.subject.meshThymidine-
dc.titleIn vitro labelling studies and the measurement of epithelial cell proliferative activity in the human oral cavity.en
dc.typeArticleen
dc.contributor.departmentDepartment of Oral and Maxillofacial Surgery, The Dental School, University of Newcastle, Framlington Place, NE2 4BW, Newcastle upon Tyne, UK. peter.thomson@nd.ac.uken
dc.identifier.journalArchives of Oral Biologyen
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