Assessment of DNA damage in C6 glioma cells after antidepressant treatment using an alkaline comet assay.

2.50
Hdl Handle:
http://hdl.handle.net/10541/85762
Title:
Assessment of DNA damage in C6 glioma cells after antidepressant treatment using an alkaline comet assay.
Authors:
Slamon, N Debbie; Ward, Timothy H; Butler, John; Pentreath, Vic W
Abstract:
The effects of acute (24 h) exposure to the antidepressants amitriptyline, imipramine (both tricyclics), fluoxetine (a selective serotonin re-uptake inhibitor) and tranylcypromine (a monoamine oxidase inhibitor) on DNA damage in cultured C6 rat glioma cells were determined using an alkaline comet assay. The effects of manipulation of intracellular cyclic AMP by pretreatment with dibutyryl cyclic AMP (dBcAMP) and 3-isobutyl-1-methylxanthine (IBMX) were also studied. For fluoxetine, the effects of addition of exogenous glutathione (GSH) and pretreatment with L-buthionine sulfoximine (BSO) were also assessed. There were increases in DNA damage with increasing concentrations of antidepressants. IBMX pretreatment protected against antidepressant-induced DNA damage in C6 cells pretreated with dBcAMP. Addition of exogenous reduced GSH and BSO increased DNA damage after fluoxetine exposure. The data show that the antidepressants induce significant amounts DNA damage in C6 cells.
Affiliation:
Department of Biological Sciences, University of Salford, UK.
Citation:
Assessment of DNA damage in C6 glioma cells after antidepressant treatment using an alkaline comet assay. 2001, 75 (4):243-50 Arch. Toxicol.
Journal:
Archives of Toxicology
Issue Date:
Jun-2001
URI:
http://hdl.handle.net/10541/85762
DOI:
10.1007/s002040100228
PubMed ID:
11482523
Type:
Article
Language:
en
ISSN:
0340-5761
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorSlamon, N Debbieen
dc.contributor.authorWard, Timothy Hen
dc.contributor.authorButler, Johnen
dc.contributor.authorPentreath, Vic Wen
dc.date.accessioned2009-11-10T10:32:39Z-
dc.date.available2009-11-10T10:32:39Z-
dc.date.issued2001-06-
dc.identifier.citationAssessment of DNA damage in C6 glioma cells after antidepressant treatment using an alkaline comet assay. 2001, 75 (4):243-50 Arch. Toxicol.en
dc.identifier.issn0340-5761-
dc.identifier.pmid11482523-
dc.identifier.doi10.1007/s002040100228-
dc.identifier.urihttp://hdl.handle.net/10541/85762-
dc.description.abstractThe effects of acute (24 h) exposure to the antidepressants amitriptyline, imipramine (both tricyclics), fluoxetine (a selective serotonin re-uptake inhibitor) and tranylcypromine (a monoamine oxidase inhibitor) on DNA damage in cultured C6 rat glioma cells were determined using an alkaline comet assay. The effects of manipulation of intracellular cyclic AMP by pretreatment with dibutyryl cyclic AMP (dBcAMP) and 3-isobutyl-1-methylxanthine (IBMX) were also studied. For fluoxetine, the effects of addition of exogenous glutathione (GSH) and pretreatment with L-buthionine sulfoximine (BSO) were also assessed. There were increases in DNA damage with increasing concentrations of antidepressants. IBMX pretreatment protected against antidepressant-induced DNA damage in C6 cells pretreated with dBcAMP. Addition of exogenous reduced GSH and BSO increased DNA damage after fluoxetine exposure. The data show that the antidepressants induce significant amounts DNA damage in C6 cells.en
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subject.mesh1-Methyl-3-isobutylxanthine-
dc.subject.meshAnimals-
dc.subject.meshAntidepressive Agents, Second-Generation-
dc.subject.meshAntidepressive Agents, Tricyclic-
dc.subject.meshBucladesine-
dc.subject.meshButhionine Sulfoximine-
dc.subject.meshComet Assay-
dc.subject.meshDNA Damage-
dc.subject.meshEnzyme Inhibitors-
dc.subject.meshGlioma-
dc.subject.meshGlutathione-
dc.subject.meshPhosphodiesterase Inhibitors-
dc.subject.meshRats-
dc.subject.meshTumor Cells, Cultured-
dc.titleAssessment of DNA damage in C6 glioma cells after antidepressant treatment using an alkaline comet assay.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biological Sciences, University of Salford, UK.en
dc.identifier.journalArchives of Toxicologyen
All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.