Anti-8-oxo-2'-deoxyguanosine phage antibodies: isolation, characterization, and relationship to disease states.

2.50
Hdl Handle:
http://hdl.handle.net/10541/85759
Title:
Anti-8-oxo-2'-deoxyguanosine phage antibodies: isolation, characterization, and relationship to disease states.
Authors:
Seedhouse, C H; Margison, Geoffrey P; Hendry, Jolyon H; Hajeer, A; Embleton, Jim
Abstract:
We have used human single chain Fv (scFv) phage display antibody libraries to isolate recombinant antibodies against the DNA adduct 8-oxo-2'-deoxyguanosine (8-oxodG). One of these scFvs (175G) bound to several 8-oxodG-containing oligonucleotides whilst demonstrating no cross-reactivity with G-containing control oligonucleotides, and bound to 8-oxodG lesions introduced into DNA by treatment with methylene blue and white light. In addition, 175G inhibited the cleavage of an 8-oxodG-containing oligonucleotide by the Escherichia coli enzyme formamidopyrimidine-DNA glycosylase (Fpg). The nucleotide sequence of the 175G V(H) gene segment was 98% homologous to the published V(H) sequence of a human hybridoma derived from a patient with systemic lupus erythematosus (SLE). Sera from two SLE patients bound to damaged DNA, and this binding could be inhibited by 175G. The use of human scFv phage display libraries has thus produced a unique reagent with specificity for 8-oxodG, which may have a role in damage detection and quantitation and in modifying DNA repair activity. 175G also offers support to the hypothesis that SLE might be associated with oxidative damage to DNA.
Affiliation:
Department of Immunology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, M20 4BX, United Kingdom.
Citation:
Anti-8-oxo-2'-deoxyguanosine phage antibodies: isolation, characterization, and relationship to disease states. 2001, 280 (3):595-604 Biochem. Biophys. Res. Commun.
Journal:
Biochemical and Biophysical Research Communications
Issue Date:
26-Jan-2001
URI:
http://hdl.handle.net/10541/85759
DOI:
10.1006/bbrc.2000.4170
PubMed ID:
11162561
Type:
Article
Language:
en
ISSN:
0006-291X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorSeedhouse, C Hen
dc.contributor.authorMargison, Geoffrey Pen
dc.contributor.authorHendry, Jolyon Hen
dc.contributor.authorHajeer, Aen
dc.contributor.authorEmbleton, Jimen
dc.date.accessioned2009-11-10T10:26:49Z-
dc.date.available2009-11-10T10:26:49Z-
dc.date.issued2001-01-26-
dc.identifier.citationAnti-8-oxo-2'-deoxyguanosine phage antibodies: isolation, characterization, and relationship to disease states. 2001, 280 (3):595-604 Biochem. Biophys. Res. Commun.en
dc.identifier.issn0006-291X-
dc.identifier.pmid11162561-
dc.identifier.doi10.1006/bbrc.2000.4170-
dc.identifier.urihttp://hdl.handle.net/10541/85759-
dc.description.abstractWe have used human single chain Fv (scFv) phage display antibody libraries to isolate recombinant antibodies against the DNA adduct 8-oxo-2'-deoxyguanosine (8-oxodG). One of these scFvs (175G) bound to several 8-oxodG-containing oligonucleotides whilst demonstrating no cross-reactivity with G-containing control oligonucleotides, and bound to 8-oxodG lesions introduced into DNA by treatment with methylene blue and white light. In addition, 175G inhibited the cleavage of an 8-oxodG-containing oligonucleotide by the Escherichia coli enzyme formamidopyrimidine-DNA glycosylase (Fpg). The nucleotide sequence of the 175G V(H) gene segment was 98% homologous to the published V(H) sequence of a human hybridoma derived from a patient with systemic lupus erythematosus (SLE). Sera from two SLE patients bound to damaged DNA, and this binding could be inhibited by 175G. The use of human scFv phage display libraries has thus produced a unique reagent with specificity for 8-oxodG, which may have a role in damage detection and quantitation and in modifying DNA repair activity. 175G also offers support to the hypothesis that SLE might be associated with oxidative damage to DNA.en
dc.language.isoenen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAntibodies-
dc.subject.meshAntibody Specificity-
dc.subject.meshBase Sequence-
dc.subject.meshCloning, Molecular-
dc.subject.meshDNA Damage-
dc.subject.meshDNA Repair-
dc.subject.meshDeoxyguanosine-
dc.subject.meshHumans-
dc.subject.meshImmunoglobulin Fragments-
dc.subject.meshLupus Erythematosus, Systemic-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshOligodeoxyribonucleotides-
dc.subject.meshPeptide Library-
dc.titleAnti-8-oxo-2'-deoxyguanosine phage antibodies: isolation, characterization, and relationship to disease states.en
dc.typeArticleen
dc.contributor.departmentDepartment of Immunology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, M20 4BX, United Kingdom.en
dc.identifier.journalBiochemical and Biophysical Research Communicationsen

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