Short tandem repeat profiling provides an international reference standard for human cell lines.

2.50
Hdl Handle:
http://hdl.handle.net/10541/85729
Title:
Short tandem repeat profiling provides an international reference standard for human cell lines.
Authors:
Masters, J R; Thomson, J A; Daly-Burns, B; Reid, Y A; Dirks, W G; Packer, P; Toji, L H; Ohno, T; Tanabe, H; Arlett, C F; Kelland, L R; Harrison, M; Virmani, A; Ward, Timothy H; Ayres, K L; Debenham, P G
Abstract:
Cross-contamination between cell lines is a longstanding and frequent cause of scientific misrepresentation. Estimates from national testing services indicate that up to 36% of cell lines are of a different origin or species to that claimed. To test a standard method of cell line authentication, 253 human cell lines from banks and research institutes worldwide were analyzed by short tandem repeat profiling. The short tandem repeat profile is a simple numerical code that is reproducible between laboratories, is inexpensive, and can provide an international reference standard for every cell line. If DNA profiling of cell lines is accepted and demanded internationally, scientific misrepresentation because of cross-contamination can be largely eliminated.
Affiliation:
Institute of Urology, University College London, 3rd Floor Research Laboratories, 67 Riding House Street, London W1W 7EY, United Kingdom. J.Masters@Ucl.ac.uk
Citation:
Short tandem repeat profiling provides an international reference standard for human cell lines. 2001, 98 (14):8012-7 Proc. Natl. Acad. Sci. U.S.A.
Journal:
Proceedings of the National Academy of Sciences of the United States of America
Issue Date:
3-Jul-2001
URI:
http://hdl.handle.net/10541/85729
DOI:
10.1073/pnas.121616198
PubMed ID:
11416159
Type:
Article
Language:
en
ISSN:
0027-8424
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMasters, J Ren
dc.contributor.authorThomson, J Aen
dc.contributor.authorDaly-Burns, Ben
dc.contributor.authorReid, Y Aen
dc.contributor.authorDirks, W Gen
dc.contributor.authorPacker, Pen
dc.contributor.authorToji, L Hen
dc.contributor.authorOhno, Ten
dc.contributor.authorTanabe, Hen
dc.contributor.authorArlett, C Fen
dc.contributor.authorKelland, L Ren
dc.contributor.authorHarrison, Men
dc.contributor.authorVirmani, Aen
dc.contributor.authorWard, Timothy Hen
dc.contributor.authorAyres, K Len
dc.contributor.authorDebenham, P Gen
dc.date.accessioned2009-11-10T09:59:53Z-
dc.date.available2009-11-10T09:59:53Z-
dc.date.issued2001-07-03-
dc.identifier.citationShort tandem repeat profiling provides an international reference standard for human cell lines. 2001, 98 (14):8012-7 Proc. Natl. Acad. Sci. U.S.A.en
dc.identifier.issn0027-8424-
dc.identifier.pmid11416159-
dc.identifier.doi10.1073/pnas.121616198-
dc.identifier.urihttp://hdl.handle.net/10541/85729-
dc.description.abstractCross-contamination between cell lines is a longstanding and frequent cause of scientific misrepresentation. Estimates from national testing services indicate that up to 36% of cell lines are of a different origin or species to that claimed. To test a standard method of cell line authentication, 253 human cell lines from banks and research institutes worldwide were analyzed by short tandem repeat profiling. The short tandem repeat profile is a simple numerical code that is reproducible between laboratories, is inexpensive, and can provide an international reference standard for every cell line. If DNA profiling of cell lines is accepted and demanded internationally, scientific misrepresentation because of cross-contamination can be largely eliminated.en
dc.language.isoenen
dc.subject.meshCell Line-
dc.subject.meshGene Expression Profiling-
dc.subject.meshHumans-
dc.subject.meshReference Standards-
dc.subject.meshTandem Repeat Sequences-
dc.titleShort tandem repeat profiling provides an international reference standard for human cell lines.en
dc.typeArticleen
dc.contributor.departmentInstitute of Urology, University College London, 3rd Floor Research Laboratories, 67 Riding House Street, London W1W 7EY, United Kingdom. J.Masters@Ucl.ac.uken
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of Americaen

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