Caspase activation during spontaneous and radiation-induced apoptosis in the murine intestine.

2.50
Hdl Handle:
http://hdl.handle.net/10541/85728
Title:
Caspase activation during spontaneous and radiation-induced apoptosis in the murine intestine.
Authors:
Marshman, Emma; Ottewell, Penny; Potten, Christopher S; Watson, Alastair
Abstract:
The aim of this study was to characterize the activation of caspase-3 along the crypt/villus axis in the normal and irradiated intestine and to compare active caspase-3 expression with existing apoptosis detection techniques. Small and large intestine were removed from mice at various time points after exposure to 8 Gy gamma-radiation. Positive apoptotic cells stained with an antibody against active caspase-3, haematoxylin and eosin (H&E) or TUNEL were scored in histological sections of small and large intestinal crypts and villi. In the control intestine, active caspase-3 expression was rarely observed; however, expression was markedly increased following exposure to radiation and was predominantly confined to apoptotic bodies. Measurement of apoptosis in intestinal crypts using active caspase-3 expression gave similar results to apoptosis detected from H&E-stained sections. In the normal villus, active caspase-3 expression was observed infrequently and did not significantly increase following radiation, consistent with a lack of apoptotic body formation from H&E sections. This study indicates that caspase-3 is activated in intestinal crypts but not in villi following gamma-radiation. Active caspase-3 detection compared favourably with existing immunological techniques, suggesting that it is a suitable alternative method for apoptosis quantification.
Affiliation:
Department of Medicine, University of Liverpool, UCD Duncan Building, Daulby Street, Liverpool, L69 3GA, UK.
Citation:
Caspase activation during spontaneous and radiation-induced apoptosis in the murine intestine. 2001, 195 (3):285-92 J. Pathol.
Journal:
The Journal of Pathology
Issue Date:
Oct-2001
URI:
http://hdl.handle.net/10541/85728
DOI:
10.1002/path.967
PubMed ID:
11673824
Type:
Article
Language:
en
ISSN:
0022-3417
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMarshman, Emmaen
dc.contributor.authorOttewell, Pennyen
dc.contributor.authorPotten, Christopher Sen
dc.contributor.authorWatson, Alastairen
dc.date.accessioned2009-11-10T09:59:00Z-
dc.date.available2009-11-10T09:59:00Z-
dc.date.issued2001-10-
dc.identifier.citationCaspase activation during spontaneous and radiation-induced apoptosis in the murine intestine. 2001, 195 (3):285-92 J. Pathol.en
dc.identifier.issn0022-3417-
dc.identifier.pmid11673824-
dc.identifier.doi10.1002/path.967-
dc.identifier.urihttp://hdl.handle.net/10541/85728-
dc.description.abstractThe aim of this study was to characterize the activation of caspase-3 along the crypt/villus axis in the normal and irradiated intestine and to compare active caspase-3 expression with existing apoptosis detection techniques. Small and large intestine were removed from mice at various time points after exposure to 8 Gy gamma-radiation. Positive apoptotic cells stained with an antibody against active caspase-3, haematoxylin and eosin (H&E) or TUNEL were scored in histological sections of small and large intestinal crypts and villi. In the control intestine, active caspase-3 expression was rarely observed; however, expression was markedly increased following exposure to radiation and was predominantly confined to apoptotic bodies. Measurement of apoptosis in intestinal crypts using active caspase-3 expression gave similar results to apoptosis detected from H&E-stained sections. In the normal villus, active caspase-3 expression was observed infrequently and did not significantly increase following radiation, consistent with a lack of apoptotic body formation from H&E sections. This study indicates that caspase-3 is activated in intestinal crypts but not in villi following gamma-radiation. Active caspase-3 detection compared favourably with existing immunological techniques, suggesting that it is a suitable alternative method for apoptosis quantification.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshAntibodies, Monoclonal-
dc.subject.meshApoptosis-
dc.subject.meshCaspase 3-
dc.subject.meshCaspases-
dc.subject.meshColon-
dc.subject.meshEnzyme Activation-
dc.subject.meshEosine Yellowish-(YS)-
dc.subject.meshEpithelium-
dc.subject.meshHematoxylin-
dc.subject.meshImmunohistochemistry-
dc.subject.meshIn Situ Nick-End Labeling-
dc.subject.meshIntestine, Small-
dc.subject.meshIntestines-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred Strains-
dc.subject.meshStaining and Labeling-
dc.titleCaspase activation during spontaneous and radiation-induced apoptosis in the murine intestine.en
dc.typeArticleen
dc.contributor.departmentDepartment of Medicine, University of Liverpool, UCD Duncan Building, Daulby Street, Liverpool, L69 3GA, UK.en
dc.identifier.journalThe Journal of Pathologyen

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