Caspase activation during spontaneous and radiation-induced apoptosis in the murine intestine.
Affiliation
Department of Medicine, University of Liverpool, UCD Duncan Building, Daulby Street, Liverpool, L69 3GA, UK.Issue Date
2001-10
Metadata
Show full item recordAbstract
The aim of this study was to characterize the activation of caspase-3 along the crypt/villus axis in the normal and irradiated intestine and to compare active caspase-3 expression with existing apoptosis detection techniques. Small and large intestine were removed from mice at various time points after exposure to 8 Gy gamma-radiation. Positive apoptotic cells stained with an antibody against active caspase-3, haematoxylin and eosin (H&E) or TUNEL were scored in histological sections of small and large intestinal crypts and villi. In the control intestine, active caspase-3 expression was rarely observed; however, expression was markedly increased following exposure to radiation and was predominantly confined to apoptotic bodies. Measurement of apoptosis in intestinal crypts using active caspase-3 expression gave similar results to apoptosis detected from H&E-stained sections. In the normal villus, active caspase-3 expression was observed infrequently and did not significantly increase following radiation, consistent with a lack of apoptotic body formation from H&E sections. This study indicates that caspase-3 is activated in intestinal crypts but not in villi following gamma-radiation. Active caspase-3 detection compared favourably with existing immunological techniques, suggesting that it is a suitable alternative method for apoptosis quantification.Citation
Caspase activation during spontaneous and radiation-induced apoptosis in the murine intestine. 2001, 195 (3):285-92 J. Pathol.Journal
The Journal of PathologyDOI
10.1002/path.967PubMed ID
11673824Type
ArticleLanguage
enISSN
0022-3417ae974a485f413a2113503eed53cd6c53
10.1002/path.967
Scopus Count
Collections
Related articles
- Burn-induced gut mucosal homeostasis in TCR delta receptor-deficient mice.
- Authors: Wu X, Woodside KJ, Song J, Wolf SE
- Issue date: 2004 Jan
- Comparison of immunohistochemistry for activated caspase-3 and cleaved cytokeratin 18 with the TUNEL method for quantification of apoptosis in histological sections of PC-3 subcutaneous xenografts.
- Authors: Duan WR, Garner DS, Williams SD, Funckes-Shippy CL, Spath IS, Blomme EA
- Issue date: 2003 Feb
- General suitability of techniques for in situ detection of apoptosis in small intestinal epithelium.
- Authors: Groos S, Reale E, Luciano L
- Issue date: 2003 Jun
- Induction of apoptosis before shedding of human intestinal epithelial cells.
- Authors: Grossmann J, Walther K, Artinger M, Rümmele P, Woenckhaus M, Schölmerich J
- Issue date: 2002 Jun
- Mechanisms underlying the radioprotective effect of histamine on small intestine.
- Authors: Medina VA, Croci M, Mohamad NA, Massari N, Garbarino G, Cricco GP, Núñez MA, Martín GA, Crescenti EJ, Bergoc RM, Rivera ES
- Issue date: 2007 Oct