The RGS domain-containing fission yeast protein, Rgs1p, regulates pheromone signalling and is required for mating.

2.50
Hdl Handle:
http://hdl.handle.net/10541/85726
Title:
The RGS domain-containing fission yeast protein, Rgs1p, regulates pheromone signalling and is required for mating.
Authors:
Pereira, Paulo S; Jones, Nic
Abstract:
BACKGROUND: When nutritionally starved, the fission yeast Schizosaccharomyces pombe enters a cell differentiation process which leads to mating and meiosis. The Ste11 protein is a key regulator of this differentiation pathway, activating the transcription of mating and meiotic genes upon starvation. RESULTS: Here, we describe rgs1, a member of the Regulator of G-protein Signalling (RGS) family, as a novel Ste11 target gene. rgs1 expression requires both an Ste11-mediated nitrogen starvation signal and the pheromone-induced activation of the Byr2/Byr1/Spk1 MAPK pathway. We show that rgs1 deletion results in a sensitivity to pheromone and in a mating defect. Deltargs1 cells initiate the mating pathway normally, undergoing sexual agglutination and G1 arrest, while inducing pheromone-dependent transcription, but then fail to fuse with a mating partner while elongating abnormal conjugation tubes. Endogenous Rgs1 tagged with GFP localizes to the nucleus and cytoplasm, and this localization pattern is not altered during pheromone treatment. Importantly, Rgs1 function requires its C-terminal RGS domain, as well as a central DEP domain and a novel homology domain present in its N-terminal region (Fungal-DR domain). CONCLUSIONS: These results demonstrate that rgs1 expression requires nutritional starvation and pheromone signalling. Rgs1 negatively regulates pheromone signalling during mating, acting in a negative feedback loop that is essential for the mating process.
Affiliation:
Imperial Cancer Research Fund, Gene Regulation Laboratory, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.
Citation:
The RGS domain-containing fission yeast protein, Rgs1p, regulates pheromone signalling and is required for mating. 2001, 6 (9):789-802 Genes Cells
Journal:
Genes to Cells
Issue Date:
Sep-2001
URI:
http://hdl.handle.net/10541/85726
PubMed ID:
11554925
Type:
Article
Language:
en
ISSN:
1356-9597
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorPereira, Paulo Sen
dc.contributor.authorJones, Nicen
dc.date.accessioned2009-11-10T09:55:49Z-
dc.date.available2009-11-10T09:55:49Z-
dc.date.issued2001-09-
dc.identifier.citationThe RGS domain-containing fission yeast protein, Rgs1p, regulates pheromone signalling and is required for mating. 2001, 6 (9):789-802 Genes Cellsen
dc.identifier.issn1356-9597-
dc.identifier.pmid11554925-
dc.identifier.urihttp://hdl.handle.net/10541/85726-
dc.description.abstractBACKGROUND: When nutritionally starved, the fission yeast Schizosaccharomyces pombe enters a cell differentiation process which leads to mating and meiosis. The Ste11 protein is a key regulator of this differentiation pathway, activating the transcription of mating and meiotic genes upon starvation. RESULTS: Here, we describe rgs1, a member of the Regulator of G-protein Signalling (RGS) family, as a novel Ste11 target gene. rgs1 expression requires both an Ste11-mediated nitrogen starvation signal and the pheromone-induced activation of the Byr2/Byr1/Spk1 MAPK pathway. We show that rgs1 deletion results in a sensitivity to pheromone and in a mating defect. Deltargs1 cells initiate the mating pathway normally, undergoing sexual agglutination and G1 arrest, while inducing pheromone-dependent transcription, but then fail to fuse with a mating partner while elongating abnormal conjugation tubes. Endogenous Rgs1 tagged with GFP localizes to the nucleus and cytoplasm, and this localization pattern is not altered during pheromone treatment. Importantly, Rgs1 function requires its C-terminal RGS domain, as well as a central DEP domain and a novel homology domain present in its N-terminal region (Fungal-DR domain). CONCLUSIONS: These results demonstrate that rgs1 expression requires nutritional starvation and pheromone signalling. Rgs1 negatively regulates pheromone signalling during mating, acting in a negative feedback loop that is essential for the mating process.en
dc.language.isoenen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshBase Sequence-
dc.subject.meshBlotting, Northern-
dc.subject.meshConjugation, Genetic-
dc.subject.meshDNA Primers-
dc.subject.meshFungal Proteins-
dc.subject.meshGreen Fluorescent Proteins-
dc.subject.meshHumans-
dc.subject.meshLuminescent Proteins-
dc.subject.meshMitogen-Activated Protein Kinase Kinases-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshPeptides-
dc.subject.meshPheromones-
dc.subject.meshPolymerase Chain Reaction-
dc.subject.meshRGS Proteins-
dc.subject.meshRNA, Messenger-
dc.subject.meshSchizosaccharomyces-
dc.subject.meshSchizosaccharomyces pombe Proteins-
dc.subject.meshSequence Homology, Amino Acid-
dc.subject.meshSignal Transduction-
dc.subject.meshTranscription Factors-
dc.titleThe RGS domain-containing fission yeast protein, Rgs1p, regulates pheromone signalling and is required for mating.en
dc.typeArticleen
dc.contributor.departmentImperial Cancer Research Fund, Gene Regulation Laboratory, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.en
dc.identifier.journalGenes to Cellsen

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