Tumorigenic target cell regions in bone marrow studied by localized dosimetry of 239Pu, 241Am and 233U in the mouse femur.

2.50
Hdl Handle:
http://hdl.handle.net/10541/85716
Title:
Tumorigenic target cell regions in bone marrow studied by localized dosimetry of 239Pu, 241Am and 233U in the mouse femur.
Authors:
Lord, Brian I; Austin, A L; Ellender, Michele; Haines, J W; Harrison, J D
Abstract:
PURPOSE: To study the temporal change in microdistribution of plutonium-239, americium-241 and uranium-233 in the mouse distal femur and to compare and combine calculated radiation doses with those obtained previously for the femoral shaft. Also, to relate doses to relative risks of osteosarcoma and acute myeloid leukaemia. MATERIALS AND METHODS: Computer-based image analysis of neutron-induced and alpha-track autoradiographs of sections of mouse femora was used to quantify the microdistribution of (239)Pu, (241)Am and (233)U from 1 to 448 days after intraperitoneal injection. Localized dose-rates and cumulative doses over this period were calculated for different regions of the marrow spaces in trabecular bone. The results were then combined with previous data for doses to the cortical marrow of the femoral shaft. A morphometric analysis of the distal femur was carried out. RESULTS: Initial deposition on endosteal surfaces and dose-rates near to the trabecular surfaces at 1 day were two to four times greater than corresponding results for cortical bone. Burial was most rapid for (233)U, about twice the rate in cortical bone. As in cortical bone, subsequent uptake into the marrow was seen for (239)Pu and (241)Am but not (233)U. Cumulative doses to 448 days for different regions of trabecular marrow were greater than corresponding values for cortical marrow for each radionuclide. Combined doses reflected the greater overall volume of cortical marrow. CONCLUSIONS: Cumulative radiation doses to the 10 microm thick band of marrow adjacent to all endosteal surfaces were in the ratio of approximately 7:3:1 for (239)Pu:(241)Am:(233)U. This ratio is not inconsistent with observed incidences of osteosarcoma induction by the three nuclides. Analysis of doses to different depths of marrow, however, showed that although ratios were probably not significantly different to that for a 10 microm depth, better correlations with osteosarcomagenic risk were obtained with 20-40 microm depths. For acute myeloid leukaemia, the closest relationship between relative risk and doses was obtained by considering only the central 5-10% of marrow, which gave a dose ratio of approximately 12:11:1 for (239)Pu:(241)Am:(233)U respectively.
Affiliation:
CRC Experimental Haematology Group, Paterson Institute Cancer Research, Christie Hospital NHS Trust, Wilmslow Manchester M20 4BX, UK. blord@picr.man.ac.uk
Citation:
Tumorigenic target cell regions in bone marrow studied by localized dosimetry of 239Pu, 241Am and 233U in the mouse femur. 2001, 77 (6):665-78 Int. J. Radiat. Biol.
Journal:
International Journal of Radiation Biology
Issue Date:
Jun-2001
URI:
http://hdl.handle.net/10541/85716
DOI:
10.1080/095530000110045944
PubMed ID:
11403706
Type:
Article
Language:
en
ISSN:
0955-3002
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLord, Brian Ien
dc.contributor.authorAustin, A Len
dc.contributor.authorEllender, Micheleen
dc.contributor.authorHaines, J Wen
dc.contributor.authorHarrison, J Den
dc.date.accessioned2009-11-10T09:28:28Z-
dc.date.available2009-11-10T09:28:28Z-
dc.date.issued2001-06-
dc.identifier.citationTumorigenic target cell regions in bone marrow studied by localized dosimetry of 239Pu, 241Am and 233U in the mouse femur. 2001, 77 (6):665-78 Int. J. Radiat. Biol.en
dc.identifier.issn0955-3002-
dc.identifier.pmid11403706-
dc.identifier.doi10.1080/095530000110045944-
dc.identifier.urihttp://hdl.handle.net/10541/85716-
dc.description.abstractPURPOSE: To study the temporal change in microdistribution of plutonium-239, americium-241 and uranium-233 in the mouse distal femur and to compare and combine calculated radiation doses with those obtained previously for the femoral shaft. Also, to relate doses to relative risks of osteosarcoma and acute myeloid leukaemia. MATERIALS AND METHODS: Computer-based image analysis of neutron-induced and alpha-track autoradiographs of sections of mouse femora was used to quantify the microdistribution of (239)Pu, (241)Am and (233)U from 1 to 448 days after intraperitoneal injection. Localized dose-rates and cumulative doses over this period were calculated for different regions of the marrow spaces in trabecular bone. The results were then combined with previous data for doses to the cortical marrow of the femoral shaft. A morphometric analysis of the distal femur was carried out. RESULTS: Initial deposition on endosteal surfaces and dose-rates near to the trabecular surfaces at 1 day were two to four times greater than corresponding results for cortical bone. Burial was most rapid for (233)U, about twice the rate in cortical bone. As in cortical bone, subsequent uptake into the marrow was seen for (239)Pu and (241)Am but not (233)U. Cumulative doses to 448 days for different regions of trabecular marrow were greater than corresponding values for cortical marrow for each radionuclide. Combined doses reflected the greater overall volume of cortical marrow. CONCLUSIONS: Cumulative radiation doses to the 10 microm thick band of marrow adjacent to all endosteal surfaces were in the ratio of approximately 7:3:1 for (239)Pu:(241)Am:(233)U. This ratio is not inconsistent with observed incidences of osteosarcoma induction by the three nuclides. Analysis of doses to different depths of marrow, however, showed that although ratios were probably not significantly different to that for a 10 microm depth, better correlations with osteosarcomagenic risk were obtained with 20-40 microm depths. For acute myeloid leukaemia, the closest relationship between relative risk and doses was obtained by considering only the central 5-10% of marrow, which gave a dose ratio of approximately 12:11:1 for (239)Pu:(241)Am:(233)U respectively.en
dc.language.isoenen
dc.subjectAcute Myeloid Leukaemiaen
dc.subjectRadiation-Induced Canceren
dc.subject.meshAmericium-
dc.subject.meshAnimals-
dc.subject.meshAutoradiography-
dc.subject.meshBone Marrow-
dc.subject.meshDose-Response Relationship, Radiation-
dc.subject.meshFemur-
dc.subject.meshHumans-
dc.subject.meshLeukemia, Myeloid, Acute-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred CBA-
dc.subject.meshNeoplasms, Radiation-Induced-
dc.subject.meshOsteosarcoma-
dc.subject.meshPlutonium-
dc.subject.meshRadiometry-
dc.subject.meshRisk Factors-
dc.subject.meshTissue Distribution-
dc.subject.meshUranium-
dc.titleTumorigenic target cell regions in bone marrow studied by localized dosimetry of 239Pu, 241Am and 233U in the mouse femur.en
dc.typeArticleen
dc.contributor.departmentCRC Experimental Haematology Group, Paterson Institute Cancer Research, Christie Hospital NHS Trust, Wilmslow Manchester M20 4BX, UK. blord@picr.man.ac.uken
dc.identifier.journalInternational Journal of Radiation Biologyen
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