2.50
Hdl Handle:
http://hdl.handle.net/10541/85615
Title:
Cyclin-mediated export of human Orc1.
Authors:
Laman, Heike; Peters, Gordon; Jones, Nic
Abstract:
Viral cyclin/cdk6 complexes interact with and phosphorylate human Orc1, a component of the origin recognition complex (ORC) that functions in DNA replication. Here we assess the effect that viral cyclin has on the intracellular location of human Orc1, which is present in both nuclear and cytoplasmic pools. Overexpression of K cyclin or cyclin A results in Crm1-dependent export of Orc1 to the cytoplasm, and this process is dependent on the phosphorylation status of several cdk target sites in Orc1. These findings support a model where S phase promoting cyclin activity drives the export of a component of replication complexes.
Affiliation:
Molecular Oncology Laboratory, Gene Regulation Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London, WC2A 3PX, United Kingdom.
Citation:
Cyclin-mediated export of human Orc1. 2001, 271 (2):230-7 Exp. Cell Res.
Journal:
Experimental Cell Research
Issue Date:
10-Dec-2001
URI:
http://hdl.handle.net/10541/85615
DOI:
10.1006/excr.2001.5360
PubMed ID:
11716535
Type:
Article
Language:
en
ISSN:
0014-4827
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLaman, Heikeen
dc.contributor.authorPeters, Gordonen
dc.contributor.authorJones, Nicen
dc.date.accessioned2009-11-06T16:53:33Z-
dc.date.available2009-11-06T16:53:33Z-
dc.date.issued2001-12-10-
dc.identifier.citationCyclin-mediated export of human Orc1. 2001, 271 (2):230-7 Exp. Cell Res.en
dc.identifier.issn0014-4827-
dc.identifier.pmid11716535-
dc.identifier.doi10.1006/excr.2001.5360-
dc.identifier.urihttp://hdl.handle.net/10541/85615-
dc.description.abstractViral cyclin/cdk6 complexes interact with and phosphorylate human Orc1, a component of the origin recognition complex (ORC) that functions in DNA replication. Here we assess the effect that viral cyclin has on the intracellular location of human Orc1, which is present in both nuclear and cytoplasmic pools. Overexpression of K cyclin or cyclin A results in Crm1-dependent export of Orc1 to the cytoplasm, and this process is dependent on the phosphorylation status of several cdk target sites in Orc1. These findings support a model where S phase promoting cyclin activity drives the export of a component of replication complexes.en
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAntineoplastic Agents-
dc.subject.meshAspartic Acid-
dc.subject.meshCell Compartmentation-
dc.subject.meshCell Nucleus-
dc.subject.meshCyclin A-
dc.subject.meshCyclin E-
dc.subject.meshCyclin-Dependent Kinases-
dc.subject.meshCyclins-
dc.subject.meshCytoplasm-
dc.subject.meshDNA Replication-
dc.subject.meshDNA-Binding Proteins-
dc.subject.meshFatty Acids, Unsaturated-
dc.subject.meshGene Expression Regulation-
dc.subject.meshGenetic Vectors-
dc.subject.meshGreen Fluorescent Proteins-
dc.subject.meshHumans-
dc.subject.meshIndicators and Reagents-
dc.subject.meshLuminescent Proteins-
dc.subject.meshMutation-
dc.subject.meshOrigin Recognition Complex-
dc.subject.meshPhosphorylation-
dc.subject.meshProtein Transport-
dc.subject.meshS Phase-
dc.subject.meshTumor Cells, Cultured-
dc.subject.meshViral Proteins-
dc.titleCyclin-mediated export of human Orc1.en
dc.typeArticleen
dc.contributor.departmentMolecular Oncology Laboratory, Gene Regulation Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London, WC2A 3PX, United Kingdom.en
dc.identifier.journalExperimental Cell Researchen

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