Steps of nuclear pore complex disassembly and reassembly during mitosis in early Drosophila embryos.

2.50
Hdl Handle:
http://hdl.handle.net/10541/85608
Title:
Steps of nuclear pore complex disassembly and reassembly during mitosis in early Drosophila embryos.
Authors:
Kiseleva, Elena; Rutherford, Sandra A; Cotter, Laura A; Allen, Terence D; Goldberg, Martin W
Abstract:
The mechanisms of nuclear pore complex (NPC) assembly and disassembly during mitosis in vivo are not well defined. To address this and to identify the steps of the NPC disassembly and assembly, we investigated Drosophila embryo nuclear structure at the syncytial stage of early development using field emission scanning electron microscopy (FESEM), a high resolution surface imaging technique, and transmission electron microscopy. Nuclear division in syncytial embryos is characterized by semi-closed mitosis, during which the nuclear membranes are ruptured only at the polar regions and are arranged into an inner double membrane surrounded by an additional 'spindle envelope'. FESEM analysis of the steps of this process as viewed on the surface of the dividing nucleus confirm our previous in vitro model for the assembly of the NPCs via a series of structural intermediates, showing for the first time a temporal progression from one intermediate to the next. Nascent NPCs initially appear to form at the site of fusion between the mitotic nuclear envelope and the overlying spindle membrane. A model for NPC disassembly is offered that starts with the release of the central transporter and the removal of the cytoplasmic ring subunits before the star ring.
Affiliation:
CRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital, Wilmslow Road, Manchester, M20 9BX, UK.
Citation:
Steps of nuclear pore complex disassembly and reassembly during mitosis in early Drosophila embryos. 2001, 114 (Pt 20):3607-18 J. Cell. Sci.
Journal:
Journal of Cell Science
Issue Date:
Oct-2001
URI:
http://hdl.handle.net/10541/85608
PubMed ID:
11707513
Type:
Article
Language:
en
ISSN:
0021-9533
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorKiseleva, Elenaen
dc.contributor.authorRutherford, Sandra Aen
dc.contributor.authorCotter, Laura Aen
dc.contributor.authorAllen, Terence Den
dc.contributor.authorGoldberg, Martin Wen
dc.date.accessioned2009-11-06T16:50:13Z-
dc.date.available2009-11-06T16:50:13Z-
dc.date.issued2001-10-
dc.identifier.citationSteps of nuclear pore complex disassembly and reassembly during mitosis in early Drosophila embryos. 2001, 114 (Pt 20):3607-18 J. Cell. Sci.en
dc.identifier.issn0021-9533-
dc.identifier.pmid11707513-
dc.identifier.urihttp://hdl.handle.net/10541/85608-
dc.description.abstractThe mechanisms of nuclear pore complex (NPC) assembly and disassembly during mitosis in vivo are not well defined. To address this and to identify the steps of the NPC disassembly and assembly, we investigated Drosophila embryo nuclear structure at the syncytial stage of early development using field emission scanning electron microscopy (FESEM), a high resolution surface imaging technique, and transmission electron microscopy. Nuclear division in syncytial embryos is characterized by semi-closed mitosis, during which the nuclear membranes are ruptured only at the polar regions and are arranged into an inner double membrane surrounded by an additional 'spindle envelope'. FESEM analysis of the steps of this process as viewed on the surface of the dividing nucleus confirm our previous in vitro model for the assembly of the NPCs via a series of structural intermediates, showing for the first time a temporal progression from one intermediate to the next. Nascent NPCs initially appear to form at the site of fusion between the mitotic nuclear envelope and the overlying spindle membrane. A model for NPC disassembly is offered that starts with the release of the central transporter and the removal of the cytoplasmic ring subunits before the star ring.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Nucleus-
dc.subject.meshDrosophila melanogaster-
dc.subject.meshFemale-
dc.subject.meshMicroscopy, Electron-
dc.subject.meshMitosis-
dc.subject.meshNuclear Pore-
dc.subject.meshTime Factors-
dc.titleSteps of nuclear pore complex disassembly and reassembly during mitosis in early Drosophila embryos.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital, Wilmslow Road, Manchester, M20 9BX, UK.en
dc.identifier.journalJournal of Cell Scienceen

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