CCR1 chemokine receptor expression isolates erythroid from granulocyte-macrophage progenitors.

2.50
Hdl Handle:
http://hdl.handle.net/10541/85589
Title:
CCR1 chemokine receptor expression isolates erythroid from granulocyte-macrophage progenitors.
Authors:
De Wynter, Erika A; Heyworth, Clare M; Mukaida, Naofumi; Jaworska, Ewa; Weffort-Santos, Almeriane; Matushima, Kouji; Testa, Nydia G
Abstract:
Simple methods that separate progenitor cells of different hemopoietic lineages would facilitate studies on lineage commitment and differentiation. We used an antibody specific for the chemokine receptor CCR1 to examine mononuclear cells isolated from cord blood samples. When CD34(+) cells were separated into CD34(+)CCR1(+) and CD34(+)CCR1(-) cells and plated in colony-forming assays, the granulocyte/macrophage progenitors were found almost exclusively in the CD34(+)CCR1(+) cells. In contrast, the CD34(+)CCR1(-) cells contained the majority of the erythroid progenitors. There was a highly significant difference (P<0.002) in the total percentage distribution of both granulocyte-macrophage colony-forming cells and erythroid burst-forming units between the two populations. This is the first report of separation of erythroid progenitors from granulocyte/macrophage progenitors using a chemokine receptor antibody in cord blood samples. These results suggest that at the clonogenic progenitor cell stage the expression of CCR1 might be lineage-specific. This method should prove useful for studies on erythroid progenitor and granulocyte/macrophage differentiation.
Affiliation:
Paterson Institute for Cancer Research, Manchester, United Kingdom. medeadw@leeds.ac.uk
Citation:
CCR1 chemokine receptor expression isolates erythroid from granulocyte-macrophage progenitors. 2001, 70 (3):455-60 J. Leukoc. Biol.
Journal:
Journal of Leukocyte Biology
Issue Date:
Sep-2001
URI:
http://hdl.handle.net/10541/85589
PubMed ID:
11527996
Type:
Article
Language:
en
ISSN:
0741-5400
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorDe Wynter, Erika Aen
dc.contributor.authorHeyworth, Clare Men
dc.contributor.authorMukaida, Naofumien
dc.contributor.authorJaworska, Ewaen
dc.contributor.authorWeffort-Santos, Almerianeen
dc.contributor.authorMatushima, Koujien
dc.contributor.authorTesta, Nydia Gen
dc.date.accessioned2009-11-06T16:39:32Z-
dc.date.available2009-11-06T16:39:32Z-
dc.date.issued2001-09-
dc.identifier.citationCCR1 chemokine receptor expression isolates erythroid from granulocyte-macrophage progenitors. 2001, 70 (3):455-60 J. Leukoc. Biol.en
dc.identifier.issn0741-5400-
dc.identifier.pmid11527996-
dc.identifier.urihttp://hdl.handle.net/10541/85589-
dc.description.abstractSimple methods that separate progenitor cells of different hemopoietic lineages would facilitate studies on lineage commitment and differentiation. We used an antibody specific for the chemokine receptor CCR1 to examine mononuclear cells isolated from cord blood samples. When CD34(+) cells were separated into CD34(+)CCR1(+) and CD34(+)CCR1(-) cells and plated in colony-forming assays, the granulocyte/macrophage progenitors were found almost exclusively in the CD34(+)CCR1(+) cells. In contrast, the CD34(+)CCR1(-) cells contained the majority of the erythroid progenitors. There was a highly significant difference (P<0.002) in the total percentage distribution of both granulocyte-macrophage colony-forming cells and erythroid burst-forming units between the two populations. This is the first report of separation of erythroid progenitors from granulocyte/macrophage progenitors using a chemokine receptor antibody in cord blood samples. These results suggest that at the clonogenic progenitor cell stage the expression of CCR1 might be lineage-specific. This method should prove useful for studies on erythroid progenitor and granulocyte/macrophage differentiation.en
dc.language.isoenen
dc.subjectFoetal Blooden
dc.subject.meshAntibodies-
dc.subject.meshAntigens, CD34-
dc.subject.meshBiological Markers-
dc.subject.meshCell Culture Techniques-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Lineage-
dc.subject.meshCells, Cultured-
dc.subject.meshChemokine CCL4-
dc.subject.meshColony-Forming Units Assay-
dc.subject.meshCulture Media, Serum-Free-
dc.subject.meshErythroid Precursor Cells-
dc.subject.meshFetal Blood-
dc.subject.meshFlow Cytometry-
dc.subject.meshGranulocytes-
dc.subject.meshHumans-
dc.subject.meshMacrophage Inflammatory Proteins-
dc.subject.meshMacrophages-
dc.subject.meshMyeloid Progenitor Cells-
dc.subject.meshRNA, Messenger-
dc.subject.meshReceptors, CCR1-
dc.subject.meshReceptors, Chemokine-
dc.titleCCR1 chemokine receptor expression isolates erythroid from granulocyte-macrophage progenitors.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Manchester, United Kingdom. medeadw@leeds.ac.uken
dc.identifier.journalJournal of Leukocyte Biologyen
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