The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins.

2.50
Hdl Handle:
http://hdl.handle.net/10541/85549
Title:
The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins.
Authors:
Walther, Tobias C; Fornerod, Maarten; Pickersgill, Helen; Goldberg, Martin W; Allen, Terence D; Mattaj, Iain W
Abstract:
The nuclear pore complex (NPC) is a large proteinaceous structure through which bidirectional transport of macromolecules across the nuclear envelope (NE) takes place. Nup153 is a peripheral NPC component that has been implicated in protein and RNP transport and in the interaction of NPCs with the nuclear lamina. Here, Nup153 is localized by immunogold electron microscopy to a position on the nuclear ring of the NPC. Nuclear reconstitution is used to investigate the role of Nup153 in nucleo- cytoplasmic transport and NPC architecture. NPCs assembled in the absence of Nup153 lacked several nuclear basket components, were unevenly distributed in the NE and, unlike wild-type NPCs, were mobile within the NE. Importin alpha/beta-mediated protein import into the nucleus was strongly reduced in the absence of Nup153, while transportin-mediated import was unaffected. This was due to a reduction in import complex translocation rather than to defective receptor recycling. Our results therefore reveal functions for Nup153 in NPC assembly, in anchoring NPCs within the NE and in mediating specific nuclear import events.
Affiliation:
European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Citation:
The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins. 2001, 20 (20):5703-14 EMBO J.
Journal:
The EMBO Journal
Issue Date:
15-Oct-2001
URI:
http://hdl.handle.net/10541/85549
DOI:
10.1093/emboj/20.20.5703
PubMed ID:
11598013
Type:
Article
Language:
en
ISSN:
0261-4189
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWalther, Tobias Cen
dc.contributor.authorFornerod, Maartenen
dc.contributor.authorPickersgill, Helenen
dc.contributor.authorGoldberg, Martin Wen
dc.contributor.authorAllen, Terence Den
dc.contributor.authorMattaj, Iain Wen
dc.date.accessioned2009-11-06T14:52:12Z-
dc.date.available2009-11-06T14:52:12Z-
dc.date.issued2001-10-15-
dc.identifier.citationThe nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins. 2001, 20 (20):5703-14 EMBO J.en
dc.identifier.issn0261-4189-
dc.identifier.pmid11598013-
dc.identifier.doi10.1093/emboj/20.20.5703-
dc.identifier.urihttp://hdl.handle.net/10541/85549-
dc.description.abstractThe nuclear pore complex (NPC) is a large proteinaceous structure through which bidirectional transport of macromolecules across the nuclear envelope (NE) takes place. Nup153 is a peripheral NPC component that has been implicated in protein and RNP transport and in the interaction of NPCs with the nuclear lamina. Here, Nup153 is localized by immunogold electron microscopy to a position on the nuclear ring of the NPC. Nuclear reconstitution is used to investigate the role of Nup153 in nucleo- cytoplasmic transport and NPC architecture. NPCs assembled in the absence of Nup153 lacked several nuclear basket components, were unevenly distributed in the NE and, unlike wild-type NPCs, were mobile within the NE. Importin alpha/beta-mediated protein import into the nucleus was strongly reduced in the absence of Nup153, while transportin-mediated import was unaffected. This was due to a reduction in import complex translocation rather than to defective receptor recycling. Our results therefore reveal functions for Nup153 in NPC assembly, in anchoring NPCs within the NE and in mediating specific nuclear import events.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCattle-
dc.subject.meshFemale-
dc.subject.meshHeterogeneous-Nuclear Ribonucleoproteins-
dc.subject.meshHumans-
dc.subject.meshImmunohistochemistry-
dc.subject.meshKaryopherins-
dc.subject.meshMacromolecular Substances-
dc.subject.meshMale-
dc.subject.meshMicroscopy, Immunoelectron-
dc.subject.meshNuclear Pore-
dc.subject.meshNuclear Pore Complex Proteins-
dc.subject.meshNuclear Proteins-
dc.subject.meshOocytes-
dc.subject.meshPhosphoproteins-
dc.subject.meshProtein Transport-
dc.subject.meshRecombinant Fusion Proteins-
dc.subject.meshRibonucleoproteins-
dc.subject.meshSerum Albumin, Bovine-
dc.subject.meshXenopus laevis-
dc.titleThe nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins.en
dc.typeArticleen
dc.contributor.departmentEuropean Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.en
dc.identifier.journalThe EMBO Journalen

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