Endocan is a novel chondroitin sulfate/dermatan sulfate proteoglycan that promotes hepatocyte growth factor/scatter factor mitogenic activity.

2.50
Hdl Handle:
http://hdl.handle.net/10541/85528
Title:
Endocan is a novel chondroitin sulfate/dermatan sulfate proteoglycan that promotes hepatocyte growth factor/scatter factor mitogenic activity.
Authors:
Béchard, David; Gentina, Thaibaut; Delehedde, Maryse; Scherpereel, Arnaud; Lyon, Malcolm; Aumercier, Marc; Vazeux, Rosemay; Richet, Colette; Degand, Pierre; Jude, Brigitte; Janin, Anne; Fernig, David G; Tonnel, Andre-Bernard; Lassalle, Philippe
Abstract:
Proteoglycans that modulate the activities of growth factors, chemokines, and coagulation factors regulate in turn the vascular endothelium with respect to processes such as inflammation, hemostasis, and angiogenesis. Endothelial cell-specific molecule-1 is mainly expressed by endothelial cells and regulated by pro-inflammatory cytokines (Lassalle, P., Molet, S., Janin, A., Heyden, J. V., Tavernier, J., Fiers, W., Devos, R., and Tonnel, A. B. (1996) J. Biol. Chem. 271, 20458-20464). We demonstrate that this molecule is secreted as a soluble dermatan sulfate (DS) proteoglycan. This proteoglycan represents the major form either secreted by cell lines or circulating in the human bloodstream. Because this proteoglycan is specifically secreted by endothelial cells, we propose to name it endocan. The glycosaminoglycan component of endocan consists of a single DS chain covalently attached to serine 137. Endocan dose-dependently increased the hepatocyte growth factor/scatter factor (HGF/SF)-mediated proliferation of human embryonic kidney cells, whereas the nonglycanated form of endocan did not. Moreover, DS chains purified from endocan mimicked the endocan-mediated increase of cell proliferation in the presence of HGF/SF. Overall, our results demonstrate that endocan is a novel soluble dermatan sulfate proteoglycan produced by endothelial cells. Endocan regulates HGF/SF-mediated mitogenic activity and may support the function of HGF/SF not only in embryogenesis and tissue repair after injury but also in tumor progression.
Affiliation:
INSERM U416, Institut Pasteur de Lille, 1 Rue du Dr. A Calmette, 59019 Lille, France.
Citation:
Endocan is a novel chondroitin sulfate/dermatan sulfate proteoglycan that promotes hepatocyte growth factor/scatter factor mitogenic activity. 2001, 276 (51):48341-9 J. Biol. Chem.
Journal:
The Journal of Biological Chemistry
Issue Date:
21-Dec-2001
URI:
http://hdl.handle.net/10541/85528
DOI:
10.1074/jbc.M108395200
PubMed ID:
11590178
Type:
Article
Language:
en
ISSN:
0021-9258
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBéchard, Daviden
dc.contributor.authorGentina, Thaibauten
dc.contributor.authorDelehedde, Maryseen
dc.contributor.authorScherpereel, Arnauden
dc.contributor.authorLyon, Malcolmen
dc.contributor.authorAumercier, Marcen
dc.contributor.authorVazeux, Rosemayen
dc.contributor.authorRichet, Coletteen
dc.contributor.authorDegand, Pierreen
dc.contributor.authorJude, Brigitteen
dc.contributor.authorJanin, Anneen
dc.contributor.authorFernig, David Gen
dc.contributor.authorTonnel, Andre-Bernarden
dc.contributor.authorLassalle, Philippeen
dc.date.accessioned2009-11-06T11:24:48Z-
dc.date.available2009-11-06T11:24:48Z-
dc.date.issued2001-12-21-
dc.identifier.citationEndocan is a novel chondroitin sulfate/dermatan sulfate proteoglycan that promotes hepatocyte growth factor/scatter factor mitogenic activity. 2001, 276 (51):48341-9 J. Biol. Chem.en
dc.identifier.issn0021-9258-
dc.identifier.pmid11590178-
dc.identifier.doi10.1074/jbc.M108395200-
dc.identifier.urihttp://hdl.handle.net/10541/85528-
dc.description.abstractProteoglycans that modulate the activities of growth factors, chemokines, and coagulation factors regulate in turn the vascular endothelium with respect to processes such as inflammation, hemostasis, and angiogenesis. Endothelial cell-specific molecule-1 is mainly expressed by endothelial cells and regulated by pro-inflammatory cytokines (Lassalle, P., Molet, S., Janin, A., Heyden, J. V., Tavernier, J., Fiers, W., Devos, R., and Tonnel, A. B. (1996) J. Biol. Chem. 271, 20458-20464). We demonstrate that this molecule is secreted as a soluble dermatan sulfate (DS) proteoglycan. This proteoglycan represents the major form either secreted by cell lines or circulating in the human bloodstream. Because this proteoglycan is specifically secreted by endothelial cells, we propose to name it endocan. The glycosaminoglycan component of endocan consists of a single DS chain covalently attached to serine 137. Endocan dose-dependently increased the hepatocyte growth factor/scatter factor (HGF/SF)-mediated proliferation of human embryonic kidney cells, whereas the nonglycanated form of endocan did not. Moreover, DS chains purified from endocan mimicked the endocan-mediated increase of cell proliferation in the presence of HGF/SF. Overall, our results demonstrate that endocan is a novel soluble dermatan sulfate proteoglycan produced by endothelial cells. Endocan regulates HGF/SF-mediated mitogenic activity and may support the function of HGF/SF not only in embryogenesis and tissue repair after injury but also in tumor progression.en
dc.language.isoenen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAnimals-
dc.subject.meshBlood Coagulation-
dc.subject.meshCHO Cells-
dc.subject.meshCell Line-
dc.subject.meshChondroitinases and Chondroitin Lyases-
dc.subject.meshChromatography, Gel-
dc.subject.meshCricetinae-
dc.subject.meshGlycosylation-
dc.subject.meshHepatocyte Growth Factor-
dc.subject.meshHumans-
dc.subject.meshMitogens-
dc.subject.meshMolecular Weight-
dc.subject.meshNeoplasm Proteins-
dc.subject.meshPolysaccharide-Lyases-
dc.subject.meshProteoglycans-
dc.titleEndocan is a novel chondroitin sulfate/dermatan sulfate proteoglycan that promotes hepatocyte growth factor/scatter factor mitogenic activity.en
dc.typeArticleen
dc.contributor.departmentINSERM U416, Institut Pasteur de Lille, 1 Rue du Dr. A Calmette, 59019 Lille, France.en
dc.identifier.journalThe Journal of Biological Chemistryen

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