Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation.

2.50
Hdl Handle:
http://hdl.handle.net/10541/85219
Title:
Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation.
Authors:
Elkord, Eyad
Abstract:
Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation.
Affiliation:
Clinical Immunotherapy Laboratory, Department of Medical Oncology, University of Manchester, Wilmslow Road, Manchester M204BX, UK. eelkord@picr.man.ac.uk
Citation:
Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation. 2009, 347 (1-2):87-90 J. Immunol. Methods
Journal:
Journal of Immunological Methods
Issue Date:
15-Aug-2009
URI:
http://hdl.handle.net/10541/85219
DOI:
10.1016/j.jim.2009.06.001
PubMed ID:
19538964
Type:
Article
Language:
en
ISSN:
1872-7905
Appears in Collections:
All Paterson Institute for Cancer Research; Immunology; Medical Oncology

Full metadata record

DC FieldValue Language
dc.contributor.authorElkord, Eyaden
dc.date.accessioned2009-11-03T12:44:59Z-
dc.date.available2009-11-03T12:44:59Z-
dc.date.issued2009-08-15-
dc.identifier.citationFrequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation. 2009, 347 (1-2):87-90 J. Immunol. Methodsen
dc.identifier.issn1872-7905-
dc.identifier.pmid19538964-
dc.identifier.doi10.1016/j.jim.2009.06.001-
dc.identifier.urihttp://hdl.handle.net/10541/85219-
dc.description.abstractCryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation.en
dc.language.isoenen
dc.subject.meshCD4 Lymphocyte Count-
dc.subject.meshCell Separation-
dc.subject.meshCryopreservation-
dc.subject.meshFlow Cytometry-
dc.subject.meshForkhead Transcription Factors-
dc.subject.meshHumans-
dc.subject.meshInterleukin-2 Receptor alpha Subunit-
dc.subject.meshReference Values-
dc.subject.meshT-Lymphocyte Subsets-
dc.subject.meshT-Lymphocytes, Regulatory-
dc.titleFrequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation.en
dc.typeArticleen
dc.contributor.departmentClinical Immunotherapy Laboratory, Department of Medical Oncology, University of Manchester, Wilmslow Road, Manchester M204BX, UK. eelkord@picr.man.ac.uken
dc.identifier.journalJournal of Immunological Methodsen

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