The S. pombe mitotic regulator Cut12 promotes spindle pole body activation and integration into the nuclear envelope.
Affiliation
Cancer Research UK Cell Division Group, Paterson Institute for Cancer Research, University of Manchester, Manchester M204BX, England, UK.Issue Date
2009-06-01
Metadata
Show full item recordAbstract
The fission yeast spindle pole body (SPB) comprises a cytoplasmic structure that is separated from an ill-defined nuclear component by the nuclear envelope. Upon mitotic commitment, the nuclear envelope separating these domains disperses as the two SPBs integrate into a hole that forms in the nuclear envelope. The SPB component Cut12 is linked to cell cycle control, as dominant cut12.s11 mutations suppress the mitotic commitment defect of cdc25.22 cells and elevated Cdc25 levels suppress the monopolar spindle phenotype of cut12.1 loss of function mutations. We show that the cut12.1 monopolar phenotype arises from a failure to activate and integrate the new SPB into the nuclear envelope. The activation of the old SPB was frequently delayed, and its integration into the nuclear envelope was defective, resulting in leakage of the nucleoplasm into the cytoplasm through large gaps in the nuclear envelope. We propose that these activation/integration defects arise from a local deficiency in mitosis-promoting factor activation at the new SPB.Citation
The S. pombe mitotic regulator Cut12 promotes spindle pole body activation and integration into the nuclear envelope. 2009, 185 (5):875-88 J. Cell Biol.Journal
The Journal of Cell BiologyDOI
10.1083/jcb.200812108PubMed ID
19487457Type
ArticleLanguage
enISSN
1540-8140ae974a485f413a2113503eed53cd6c53
10.1083/jcb.200812108
Scopus Count
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