The S. pombe mitotic regulator Cut12 promotes spindle pole body activation and integration into the nuclear envelope.

2.50
Hdl Handle:
http://hdl.handle.net/10541/85218
Title:
The S. pombe mitotic regulator Cut12 promotes spindle pole body activation and integration into the nuclear envelope.
Authors:
Tallada, Victor A; Tanaka, Kenji; Yanagida, Mitsuhiro; Hagan, Iain M
Abstract:
The fission yeast spindle pole body (SPB) comprises a cytoplasmic structure that is separated from an ill-defined nuclear component by the nuclear envelope. Upon mitotic commitment, the nuclear envelope separating these domains disperses as the two SPBs integrate into a hole that forms in the nuclear envelope. The SPB component Cut12 is linked to cell cycle control, as dominant cut12.s11 mutations suppress the mitotic commitment defect of cdc25.22 cells and elevated Cdc25 levels suppress the monopolar spindle phenotype of cut12.1 loss of function mutations. We show that the cut12.1 monopolar phenotype arises from a failure to activate and integrate the new SPB into the nuclear envelope. The activation of the old SPB was frequently delayed, and its integration into the nuclear envelope was defective, resulting in leakage of the nucleoplasm into the cytoplasm through large gaps in the nuclear envelope. We propose that these activation/integration defects arise from a local deficiency in mitosis-promoting factor activation at the new SPB.
Affiliation:
Cancer Research UK Cell Division Group, Paterson Institute for Cancer Research, University of Manchester, Manchester M204BX, England, UK.
Citation:
The S. pombe mitotic regulator Cut12 promotes spindle pole body activation and integration into the nuclear envelope. 2009, 185 (5):875-88 J. Cell Biol.
Journal:
The Journal of Cell Biology
Issue Date:
1-Jun-2009
URI:
http://hdl.handle.net/10541/85218
DOI:
10.1083/jcb.200812108
PubMed ID:
19487457
Type:
Article
Language:
en
ISSN:
1540-8140
Appears in Collections:
All Paterson Institute for Cancer Research; Cell Division

Full metadata record

DC FieldValue Language
dc.contributor.authorTallada, Victor Aen
dc.contributor.authorTanaka, Kenjien
dc.contributor.authorYanagida, Mitsuhiroen
dc.contributor.authorHagan, Iain Men
dc.date.accessioned2009-11-03T12:18:12Z-
dc.date.available2009-11-03T12:18:12Z-
dc.date.issued2009-06-01-
dc.identifier.citationThe S. pombe mitotic regulator Cut12 promotes spindle pole body activation and integration into the nuclear envelope. 2009, 185 (5):875-88 J. Cell Biol.en
dc.identifier.issn1540-8140-
dc.identifier.pmid19487457-
dc.identifier.doi10.1083/jcb.200812108-
dc.identifier.urihttp://hdl.handle.net/10541/85218-
dc.description.abstractThe fission yeast spindle pole body (SPB) comprises a cytoplasmic structure that is separated from an ill-defined nuclear component by the nuclear envelope. Upon mitotic commitment, the nuclear envelope separating these domains disperses as the two SPBs integrate into a hole that forms in the nuclear envelope. The SPB component Cut12 is linked to cell cycle control, as dominant cut12.s11 mutations suppress the mitotic commitment defect of cdc25.22 cells and elevated Cdc25 levels suppress the monopolar spindle phenotype of cut12.1 loss of function mutations. We show that the cut12.1 monopolar phenotype arises from a failure to activate and integrate the new SPB into the nuclear envelope. The activation of the old SPB was frequently delayed, and its integration into the nuclear envelope was defective, resulting in leakage of the nucleoplasm into the cytoplasm through large gaps in the nuclear envelope. We propose that these activation/integration defects arise from a local deficiency in mitosis-promoting factor activation at the new SPB.en
dc.language.isoenen
dc.subject.meshCell Nucleus-
dc.subject.meshCytoplasm-
dc.subject.meshMicrotubule-Associated Proteins-
dc.subject.meshMicrotubules-
dc.subject.meshMitosis-
dc.subject.meshMitotic Spindle Apparatus-
dc.subject.meshModels, Biological-
dc.subject.meshNuclear Envelope-
dc.subject.meshPhosphoproteins-
dc.subject.meshSchizosaccharomyces-
dc.subject.meshSchizosaccharomyces pombe Proteins-
dc.titleThe S. pombe mitotic regulator Cut12 promotes spindle pole body activation and integration into the nuclear envelope.en
dc.typeArticleen
dc.contributor.departmentCancer Research UK Cell Division Group, Paterson Institute for Cancer Research, University of Manchester, Manchester M204BX, England, UK.en
dc.identifier.journalThe Journal of Cell Biologyen

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